Verbascum Thapsus (Mullein) Versatile Polarity Extracts: GC-MS Analysis, Phytochemical Profiling, Anti-bacterial Potential and Anti-oxidant Activity

Nadeem, Ali; Ahmed, Bashir; Shahzad, Hira; Craker, Lyle E.; Muntean, Tudor

doi:10.5530/pj.2021.13.189

Cited by 12 publications

(7 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This substance has antimicrobial properties. [42,43], reduces Atherosclerosis, Cardioprotective,Cholesterolytic agent (cholesterol efflux) [44,45], antidiabetic [46], antiangiogenic [47], induces apoptosis [48], Induced cell cycle arrest [49], antifungal [50], antioxidant [51], antiproliferative [52], neuroprotective [45], anti-hyperglycemic [53], anti- inflammatory [54], antiparasitic [55], antibacterial [56], anticancer [57], anti-nociceptive [58], and anticonvulsant [59].…”

Section: Resultsmentioning

confidence: 99%

Determination of Marker Compounds of Senna alata from different location in East Kalimantan, Indonesia

Salam,

Laksmita,

Fernandes

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

Senna alata is a plant used for medical purposes, and its leaves have an extended history of use as a traditional herbal medicine in Indonesia. S. alata is known to contain some components of biologically active compounds and also secondary metabolites. In fact, S. alata can grow well in various locations in Indonesia, location differences can lead to differences in compound content due to differences in environmental conditions such as soil, rainfall, light intensity, and humidity. Therefore, this study aimed to analyze marker compounds of S. alata plants origin from three different location in East Kalimantan, i.e Samarinda, Samboja, and Berau. This research was conducted to estimate the compounds contained in extracts by using GC-MS analysis, and to discover relationships between different variables by using Principal Component Analysis (PCA). There are variances in the yield of secondary metabolites according on where Senna alata is grown, specifically in the riverside Nyapa Indah region and the plains of Samarinda and Samboja. Based on GC-MS test results, Phytol was the main compound in S.alata in two areas, i.e. Samarinda and Samboja. Meanwhile, in Berau showed that 1,2-Benzenedicarboxylic acid, and mono (2-ethylhexyl) ester were the main components. However, S. alata leaf extracts could be used as a good quality raw material for pharmaceutical industries, such as a laxative agent.

show abstract

Section: Resultsmentioning

confidence: 99%

Determination of Marker Compounds of Senna alata from different location in East Kalimantan, Indonesia

Salam,

Laksmita,

Fernandes

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

show abstract

“…Caffeic acid derivatives include chlorogenic acid, verbascoside, and luteolin; flavonoid- type substances include apigenin, luteolin, and their glycosidic derivatives were most known (29,30). Numerous research indicated that the Verbascum species included luteolin, apigenin, diosmin, fisetin, quercetin, aucubin, and rutin in addition to protocatechuic acid, ferulic acid, chlorogenic acid, rosmarinic acid, p-coumaric acid and caffeic acid (31)(32)(33)(34). The plant extract demonstrated lower DPPH and ABTS radical scavenging activities when compared to standard antioxidants.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant, AChE inhibitory, and anticancer effects of Verbascum thapsus extract

Na Zhang,

Ayşe Baran,

Ferzane Valioglu

et al. 2023

Cell Mol Biol (Noisy-le-grand)

View full text Add to dashboard Cite

“…Concentrations of phenols and flavonoids were identified in all extracts of N. cataria via established protocols previously explained in Nadeem et al ( 2021 ).…”

Section: Materials and Methods (Experimental)mentioning

confidence: 99%

“…Bacterial cultures ( Table 1 ) were grown on a tryptic soy broth (TSB) medium (Thermo Fisher Scientific, USA) (Nadeem et al, 2021 ). To evaluate antibacterial susceptibility testing (AST) of N. cataria extracts, three different methods were used, i.e., 96-well test, Kirby-Bauer disk diffusion, and resazurin-based well plate microdilution method.…”

Section: Materials and Methods (Experimental)mentioning

confidence: 99%

Phytochemical profiling of antimicrobial and potential antioxidant plant: Nepeta cataria

Nadeem

Shahzad²,

Ahmed³

et al. 2022

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

Traditional and phytochemical studies have confirmed the richness and diversity of medicinal plants such as Nepeta cataria (N. cataria), but more studies are needed to complete its metabolite profiling. The objective of this research was to enhance the metabolomic picture and bioactivity of N. cataria for better evaluation. Phytochemical analysis was performed by bio-guided protocols and gas chromatography-mass spectrometry (GC/MS). For this, solvents such as methanol, ethanol, water, acetone, and hexane were used to extract a wide number of chemicals. Antibacterial analysis was performed using the 96-well plate test, Kirby Bauer's disk diffusion method, and the resazurin microdilution test. Antioxidant activity was determined by the DPPH assay and radical scavenging capacity was evaluated by the oxygen radical absorbance capacity (ORAC) assay. GC/MS analysis revealed a total of 247 identified and 127 novel metabolites from all extracts of N. cataria. Water and acetone extracts had the highest identified metabolites (n = 79), whereas methanol extract was the highest in unidentified metabolites (n = 48). The most abundant phytochemicals in methanol extract were 1-isopropylcyclohex-1-ene (concentration = 27.376) and bicyclo [2.2.1] heptan-2-one (concentration = 20.437), whereas in ethanol extract, it was 9,12,15-octadecatrienoic acid (concentration = 27.308) and 1-isopropylcyclohex-1-ene (concentration = 25.854). An abundance of 2 methyl indoles, conhydrin, and coumarin was found in water extracts; a good concentration of eucalyptol was found in acetone extract; and 7,9-di-tert-butyl-1-oxaspiro is the most abundant phytochemicals in hexane extracts. The highest concentration of flavonoids and phenols were identified in hexane and methanol extracts, respectively. The highest antioxidant potential (DPPH assay) was observed in acetone extract. The ethanolic extract exhibited a two-fold higher ORAC than the methanol extract. This examination demonstrated the inhibitory effect against a set of microbes and the presence of polar and non-polar constituents of N. cataria. The results of this study provide a safe resource for the development of food, agriculture, pharmaceutical, and other industrial products upon further research validation.

show abstract

Verbascum Thapsus (Mullein) Versatile Polarity Extracts: GC-MS Analysis, Phytochemical Profiling, Anti-bacterial Potential and Anti-oxidant Activity

Cited by 12 publications

References 19 publications

Determination of Marker Compounds of Senna alata from different location in East Kalimantan, Indonesia

Determination of Marker Compounds of Senna alata from different location in East Kalimantan, Indonesia

Antioxidant, AChE inhibitory, and anticancer effects of Verbascum thapsus extract

Phytochemical profiling of antimicrobial and potential antioxidant plant: Nepeta cataria

Contact Info

Product

Resources

About