Veratryl alcohol oxidation by lignin peroxidase

Khindaria, Aditya; Yamazaki, Isao; Aust, Steven D.

doi:10.1021/bi00051a037

Cited by 78 publications

(67 citation statements)

References 28 publications

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“…Our D value of DMB in acetonitrile is close to that in CH 2 Cl 2 /CF 3 COOH reported by Fabre and co-workers [17]. In the case of veratryl alcohol (VA), the lifetime of the radical cations is too short-lived (half-life, 0.5 ms) to diffuse away from the active site of the enzyme [7,21]. Therefore, it was suggested that an enzyme-bound radical formation and it was demonstrated that the presence of an LiP compound II -VA + complex [3,7].…”

Section: Resultssupporting

confidence: 87%

“…However, there arises another question that the lifetime estimated from the first-order rate constant of the decay seems too short for the radical cation to be an efficient diffusible mediator [6]. In recent developments, it has been proposed that the formation of a complex of LiP and the cation radical extends the lifetime [7,8]. Thus, the investigation of the properties of the cation radical formed from the aromatic compounds with methoxy groups is an important subject, however, it is not easy to obtain parameters for quantitative kinetic analysis of the reaction of a typical compound such as veratryl alcohol (3,4-dimethoxybenzyl alcohol) since it undergoes a one-electron oxidation followed by a chemical reaction combined with an additional electron transfer reaction [9].…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous determination of charge number and diffusion coefficient of 2-chloro-1,4-dimethoxybenzene in acetonitrile using potential-step chronoamperometry

Yamada

2004

Bioelectrochemistry

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Section: Resultssupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of charge number and diffusion coefficient of 2-chloro-1,4-dimethoxybenzene in acetonitrile using potential-step chronoamperometry

Yamada

2004

Bioelectrochemistry

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“…This could be either EIIVAd + (k u h) or E-VAd + (k u d), or if both complexes have similar dissociation rates, either could be partially rate-limiting. This is consistent with the recently shown existence of such a complex for lignin peroxidase Compound II [16,17].…”

Section: Figure 3 Double-reciprocal Plot For Steady-state Kinetics Ofsupporting

confidence: 93%

Anionic tobacco peroxidase is active at extremely low pH: veratryl alcohol oxidation with a pH optimum of 1.8

Gazarian

Lagrimini

George

et al. 1996

Biochemical Journal

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Tobacco peroxidase (36 kDa, pI 3n5) exhibits unique catalytic and spectral properties that are modulated by pH, calcium and magnesium ions. It catalyses the oxidation of veratryl alcohol by hydrogen peroxide over a wide pH range (1n5-5n0) in the presence of these metal ions with a pH optimum of 1n8. This is the only example of a holoperoxidase described so far that is active and comparatively stable at such a low pH. The enhancement of tobacco peroxidase activity by magnesium ions is to our knowledge the first example of a magnesium-induced peroxidase activation. UV\visible spectra of tobacco peroxidase showed that the Soret band shifted and its absorption coefficient increased upon the addition of calcium or magnesium ions and on lowering

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“…58,59 Por outro lado, Popp ) como, por exemplo, pelo ácido oxálico, como descrito em detalhes mais adiante.…”

Section: áLcool Veratrílico-lipunclassified

Relevância de compostos de baixa massa molar produzidos por fungos e envolvidos na biodegradação da madeira

Arantes¹,

Milagres²

2009

Quím. Nova

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Recebido em 16/7/08; aceito em 27/1/09; publicado na web em 3/7/09 RELEVANCE OF LOW MOLECULAR WEIGHT COMPOUNDS PRODUCED BY FUNGI AND INVOLVED IN WOOD BIODEGRADATION. Over the last decade, evidences have been shown that the wood biodegradation by fungi is not only a result of the action of their enzymatic machinery but also of various low molecular weight non-enzymatic compounds, especially in fungi that promote brown and white decay, which in nature are the major wood decaying microorganisms. The present review focuses on the recent theories involving these low molecular weight compounds that act direct or synergistically with lignocellulolytic enzymes to attack the wood main macromolecular constituents, their relevance as potential degradative systems, in the overall wood biodegradation, and also outlines their potential biotechnological applications.Keywords: wood biodegradation; enzymes; low molecular weight compounds.A biodegradação de materiais lignocelulósicos, como a madeira, é um processo natural de reciclagem de matéria orgânica e o entendimento deste processo contribui com a compreensão do ciclo natural do carbono, que tem início na biossíntese dos seus constituintes a partir de H 2 O, CO 2 e energia solar e termina com a mineralização destes constituintes e liberação de CO 2 e H 2 O, em decorrência da decomposição causada principalmente por fungos.Por muito tempo o processo de biodegradação foi foco de muitos estudos devido à grande quantidade de madeira e produtos à base de madeira, que são danificados por ação de fungos, e devido à necessidade do desenvolvimento de métodos de prevenção à biodegradação menos ofensivos ao meio ambiente. Atualmente, os fungos degradadores de madeira e/ou seus sistemas degradativos têm sido intensivamente estudados em razão do elevado potencial de utilização em aplicações biotecnológicas, como na biodegradação de compostos xenobióticos, 1-3 biorremediação de solos, 1,4,5 biopolpação da madeira 6,7 e biobranqueamento de polpas celulósicas. [8][9][10] Uma vez que o desenvolvimento e a eficácia destes métodos/processos dependem principalmente do conhecimento dos mecanismos químicos e bioquímicos envolvidos na biodegradação dos constituintes da madeira, o entendimento destes mecanismos certamente irá contribuir para o desenvolvimento de novas aplicações, assim como para a melhoria das já existentes.Apesar do considerável conhecimento adquirido nas últimas dé-cadas a respeito da enzimologia da biodegradação dos constituintes da madeira, até os dias de hoje não se conhecem os mecanismos químicos e bioquímicos exatos empregados pelos fungos durante este processo. Contudo, sabe-se que o processo de biodegradação da madeira por fungos não pode ser explicado unicamente com base na ação das diversas enzimas lignocelulolíticas por eles secretadas. Dentro dessa abordagem, pesquisas recentes têm indicado uma participação expressiva de compostos de baixa massa molar (CBMM). 11,12Neste sentido, esta revisão tem como objetivo principal agrupar as diferentes teorias envolvendo CBMM produ...

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Veratryl alcohol oxidation by lignin peroxidase

Cited by 78 publications

References 28 publications

Simultaneous determination of charge number and diffusion coefficient of 2-chloro-1,4-dimethoxybenzene in acetonitrile using potential-step chronoamperometry

Simultaneous determination of charge number and diffusion coefficient of 2-chloro-1,4-dimethoxybenzene in acetonitrile using potential-step chronoamperometry

Anionic tobacco peroxidase is active at extremely low pH: veratryl alcohol oxidation with a pH optimum of 1.8

Relevância de compostos de baixa massa molar produzidos por fungos e envolvidos na biodegradação da madeira

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