Ver�nderung von Aflatoxin B1 in alkalischer L�sung

Ammoniation of aflatoxin B1. Mass spectral analysis of compounds separated by microsublimation

“…is indicative of a molecular ion. This molecular weight is in agreement with the loss of the methoxyl group of aflatoxin Di as shown in Figure5 Kiermeier and Ruffer (1974). report on the identification of such a compound after sodium hydroxide treatment of aflatoxin Bi at 100°.…”

Ammoniation of aflatoxin B1. Isolation and characterization of a product with molecular weight 206

“…The major component of the chloroform-soluble fraction of the crude ammoniation product was identified as a nonfluorescent phenol of mol wt 286 (aflatoxin Di) in which the lactone carbonyl moiety characteristic of aflatoxin Bi was lacking. Kiermeier and Ruffer (1974) also reported that a compound of mol wt 286 with a structure identical with that proposed by Lee et al for aflatoxin Di was produced by reacting aflatoxin Bi with sodium hydroxide at 100°C. This article describes the isolation and characterization of a second major ammoniation product, a nonfluorescent phenol of mol wt 206 which is similar in structure to aflatoxin Di, but lacks the cyclopentenone ring.…”

A New Laccase of Lac 2 from the White Rot Fungus Cerrena unicolor 6884 and Lac 2-Mediated Degradation of Aflatoxin B1

“…Exposure of AFB 1 to alkaline conditions caused the formation of nonfluorescent derivatives [ 45 ]. In an alkaline buffer, the lactone bond in AFB 1 will be opened when attacked by nucleophiles, especially OHˉ, and then the detectability of AFB 1 toxicity will be shielded, but the nonfluorescent compounds formed are strongly toxic based on results of the chick embryo’s test [ 12 , 46 ]. Our study showed similar results that buffers at pH 8.0, 9.0 and 10.0 were responsible for AFB 1 degradation without laccase ( Figure 3 D).…”