VentX trans-Activates p53 and p16ink4a to Regulate Cellular Senescence

Wu, Xiaoming; Gao, Hong; Ke, Weixiong; Hager, Martin H.; Xiao, Sheng; Freeman, Michael R.; Zhu, Zhongqi

doi:10.1074/jbc.m110.206078

Cited by 16 publications

(27 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, we first examined whether overexpression of VentX could exert an inhibitory role on the proliferation of THP1 cells. Consistent with our prior study (17,19), expression of VentX in THP1 cells induced apparent growth inhibition ( Fig. 2A) and G1 cell cycle arrest (Fig.…”

Section: Cd1asupporting

confidence: 78%

“…5, A-E, overexpression of VentX induced up-regulation of a wide variety of genes, such as CD1 antigens, co-stimulatory factors CD80 and CD86, HLA molecules, proinflammatory cytokines IL12, TNF␣, chemokines CCL2(MCP-1), CCL3(MIP-1␣), and CCL5 (RANTES), chemokine receptors CCR5 and CXCR4, and TLRs. In addition, the expressions of CSF1R (M-SCF receptor) and CDKN1A (p21) also increased as previously demonstrated (17,19). The results of PCR array were further verified by independent PCR reaction, using different sets of primers.…”

Section: Il-6 Mediates Ventx Regulation Of Dcs Differentiation-tosupporting

confidence: 53%

“…VentX is a direct transcriptional activator of the p53 and the p16, which are known antagonists of NFB activation of IL6 (19,34,35). Thus, VentX may regulate IL6 expression in part through its inhibitory effects on NFB binding and transactivation of IL6 promoter.…”

Section: Discussionmentioning

confidence: 99%

“…Transfection of reporter plasmid with pcDNA-VentX plasmid or control pcDNA-GFP plasmid into primary monocytes was carried out through electroporation. Reporter plasmid was also transfected into U2OS cells, which stably express tetracycline-inducible VentX (19), and reporter activity was evaluated in the absence or presence of tetracycline. 10 ng of Renilla luciferase plasmid was included for each transfection to normalize reporter activity.…”

Section: Methodsmentioning

confidence: 99%

“…Initially identified as a novel LEF/TCF associated antagonist of the canonical Wnt signaling through reverse genetic modeling of Xenopus embryogenesis, VentX was found to be a transcriptional activator of the p53/p21 and p16 ink4a /Rb tumor suppression pathways (18,19). The critical role of VentX in hematopoietic cells development was indicated by its function in controlling the proliferation and differentiation of CD34 ϩ cells and monocyte to macrophage terminal differentiation (16,17).…”

mentioning

confidence: 99%

See 4 more Smart Citations

Homeobox Transcription Factor VentX Regulates Differentiation and Maturation of Human Dendritic Cells

Gao

Bleday

et al. 2014

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Background:The transcriptional regulation of human dendritic cells (DCs) differentiation and maturation remains incompletely understood. Results: VentX knockdown impaired DCs differentiation and maturation whereas VentX overexpression counteracted the inhibitory effects of corticosteroid on DC activation. Conclusion: VentX is critical for DCs differentiation and maturation. Significance: VentX may be a novel target to modulate DCs functions and manage inflammatory diseases.

show abstract

Section: Cd1asupporting

confidence: 78%

Section: Il-6 Mediates Ventx Regulation Of Dcs Differentiation-tosupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Homeobox Transcription Factor VentX Regulates Differentiation and Maturation of Human Dendritic Cells

Gao

Bleday

et al. 2014

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

Xom induces proteolysis of β‐catenin through GSK3β‐mediated pathway

Gao

et al. 2017

FEBS Letters

Self Cite

View full text Add to dashboard Cite

The dorsal cell fate determination factor β-catenin and its antagonist, the ventral cell fate determination factor Xom, are expressed and distributed in a polarized fashion during early vertebrate embryogenesis. Ubiquitin-mediated proteolysis has been shown to control the abundance of both β-catenin and Xom. However, the mechanism of ubiquitin-mediated proteolysis in regulating dorsoventral patterning remains largely unclear. Our current study shows that Xom induces proteolysis of β-catenin through GSK3-mediated phosphorylation of Ser33/37 of β-catenin. Our findings reveal a novel pathway that regulates β-catenin stability, and suggest, for the first time, a critical function of ubiquitin-mediated proteolysis in balancing the integration of dorsal-ventral signals and the polarized distribution of β-catenin and Xom during dorsoventral axis formation.

show abstract

A risk model of 10 aging‐related genes for predicting survival and immune response in triple‐negative breast cancer

et al. 2022

View full text Add to dashboard Cite

Accumulated studies showed that the clinical significance of aging on the development and malignancy of tumors, while the relationship between aging and the prognosis, immune response in triple‐negative breast cancer (TNBC) has not been well clarified. Here, we constructed a risk model of 10 prognostic aging‐related genes (ARGs) from METABRIC database. Then, TNBC patients were classified into high‐ and low‐risk groups, the survival diversity, immune response, genomic function, and tumor mutation burden (TMB) between different risk groups were explored in METABRIC, TCGA, and GSE58812 cohorts. Results showed that patients in the high‐risk group had poorer survival outcomes compared to their counterparts (all p < 0.05), and the nomogram we established showed reliable prediction ability for survival in TNBC patients. Besides, TNBC patients with high‐risk scores had a lower expression of immune checkpoint markers and a lower fraction of activated immune cells. Furthermore, GSEA showed that Notch signaling pathway was significantly enriched in the high‐risk group. Thus, a risk model based on the aging‐related genes was developed and validated in this study, which may serve as a potential biomarker for prognosis and personalized treatment in TNBCs.

show abstract

VentX trans-Activates p53 and p16ink4a to Regulate Cellular Senescence

Cited by 16 publications

References 43 publications

Homeobox Transcription Factor VentX Regulates Differentiation and Maturation of Human Dendritic Cells

Homeobox Transcription Factor VentX Regulates Differentiation and Maturation of Human Dendritic Cells

Xom induces proteolysis of β‐catenin through GSK3β‐mediated pathway

A risk model of 10 aging‐related genes for predicting survival and immune response in triple‐negative breast cancer

Contact Info

Product

Resources

About