Ventricular myosin heavy chain isoform expression is altered in vitro in low score normal chickens

Wick, Macdonald; Velleman, Sandra G.; Coy, C. S.; McFarland, Douglas C.; Pretzman, Charles I.; St-Pierre, N.R.; Sawdy, J.C.

doi:10.1016/s1095-6433(03)00196-x

Cited by 1 publication

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“…The expression of MHC genes has been extensively used to describe the formation of the cardiac chambers (Sartore et al 1978;Sanders et al 1984;de Jong et al 1987de Jong et al ,1990Yutzey et al 1994;Agarkova et al 2000;Siedner et al 2003;Narolska et al 2005). With the identification of chamberspecific/enriched transcription factors, the expression patterns of the MHC genes gained a renewed interest, especially when ectopic expression of these transcription factors is involved in the regulation of the gene expression program related to the forming atrial or ventricular chambers (Yutzey et al 1994;Bao et al 1999;Morkin 2000;Berdougo et al 2003;Miller et al 2003;Wick et al 2003;James et al 2005). The most widely used MHC species are atrial, ventricular, and cardiac MHC (AMHC, VMHC, and CMHC, respectively).…”

Section: Discussionmentioning

confidence: 99%

Atrial and Ventricular Myosin Heavy-chain Expression in the Developing Chicken Heart: Strengths and Limitations of Non-radioactive In Situ Hybridization

Somi

Klein

Houweling

et al. 2006

J Histochem Cytochem.

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Myosin heavy-chain (MHC) isoforms are major structural components of the contractile apparatus of the heart muscle. Their spatio-temporal patterns of expression have been used as a tool to dissect cardiac development and differentiation. Although extensively investigated, controversy still exists concerning the expression patterns of atrial (AMHC), ventricular (VMHC), and cardiac myosin heavy-chain (CMHC) during development in the heart. In this study, we describe that probe length, probe concentration, and staining time in the non-radioactive in situ hybridization procedure seriously influence the observed pattern of MHC expression and the subsequent interpretation, explaining the divergent opinions in the field. Using a variety of external and internal controls for the in situ hybridization procedure, we demonstrate that both AMHC and VMHC are expressed throughout the entire heart tube during early development. During subsequent development, VMHC becomes restricted to the ventricles, whereas AMHC remains expressed in the atria, and, at substantially lower levels, is detected in the ventricles. These results are discussed in the context of methodological constraints of demonstrating patterns of gene expression. This manuscript contains online supplemental material at http://www.jhc.org . Please visit this article online to view these materials.

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