Ventricles as a major site of atrial natriuretic factor synthesis and release in cardiomyopathic hamsters with heart failure.

Thibault, Gaétan; Nemer, Mona; Drouin, Jacques; Lavigne, Jean-Pierre; Ding, Jinfeng; Charbonneau, Cindy; García, Raúl; Genest, Jacques; Jasmin, G.; Sole, Michael J.

doi:10.1161/01.res.65.1.71

Cited by 65 publications

(26 citation statements)

References 67 publications

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“…7 C). It is reported that the plasma ANP-LI concentration is elevated to 100 fmol/ml in cardiomyopathic hamsters with moderate to sever CHF (5,25). The low plasma ANP-LI concentration is, therefore, consistent with the observation that the 32-wk-old B1014.6 strain used in the present study does not manifest CHF.…”

Section: Discussionsupporting

confidence: 92%

“…3 B) (34,35). Moreover, a previous HPLC study showed that the major peak of ANP-LI in hamster plasma corresponds to the elution position of a-rat ANP (25).…”

Section: Discussionmentioning

confidence: 91%

“…Structures (-LIs) in hamster tissues using the RIA for rat ANP (5,25). In cardiomyopathic hamsters, it has been demonstrated that ANP synthesis and secretion are augmented in the ventricle and that the plasma ANP-LI concentration is increased (5, 25).…”

Section: Introductionmentioning

confidence: 99%

“…Structures of hamster ANP and BNP, however, have never been disclosed. Mammalian ANPs so far identified are 28-resi-due peptides, and their amino acid sequences are identical except for a single residue substitution at the 12th position (Ile in rodents, Met in humans) ( 1,24), which enabled investigators to detect ANP-like immunoreactivities (-LIs) in hamster tissues using the RIA for rat ANP (5,25). In cardiomyopathic hamsters, it has been demonstrated that ANP synthesis and secretion are augmented in the ventricle and that the plasma ANP-LI concentration is increased (5,25).…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning of hamster brain and atrial natriuretic peptide cDNAs. Cardiomyopathic hamsters are useful models for brain and atrial natriuretic peptides.

Tamura¹,

Ogawa²,

Itoh³

et al. 1994

J. Clin. Invest.

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IntroductionBrain and atrial natriuretic peptides (BNP and ANP) are cardiac hormones with diuretic, natriuretic, and vasodilatory activities. Cardiomyopathic hamsters are widely used animal models of heart failure. Due to the structural divergence of BNP among species, examination on pathophysiological roles of BNP using cardiomyopathic hamsters is so far impossible. We therefore isolated hamster BNP and ANP cDNAs, and investigated synthesis and secretion of these peptides in normal and cardiomyopathic hamsters. The COOH-terminal 32-residue peptide of cloned hamster preproBNP with 122 amino acids, preceded by a single arginine residue, supposedly represents hamster BNP showing < 50% homology to rat BNP. Alpha-hamster ANP, 28-residue peptide, is identical to a-rat ANP. In hamsters, BNP and ANP occur mainly in the ventricle and the atrium, respectively. The 32-wk-old hypertrophic cardiomyopathic B1014.6 strain exhibited ventricular hypertrophy. The 32-wk-old dilated cardiomyopathic B1053.58 strain remained at the stage without apparent heart failure. In B1014.6 and B1053.58 strains at this age, ventricular BNP and ANP gene expressions are augmented, and the plasma BNP concentration is elevated to 136 and 108 fmol/ml, respectively, three times greater than the elevated plasma ANP concentration, which well mimics changes of the plasma BNP and ANP concentrations in human heart failure. Cardiomyopathic hamsters, therefore, are useful models to investigate the implication of BNP in human cardiovascular diseases. (J. Clin. Invest. 1994. 94:1059-1068 Key words: cardiac hypertrophy -congestive heart failure * gene expression * radioimmunoassay * tissue distribution Address correspondence to Kazuwa Nakao, Second Division, Department of Medicine, Kyoto University Faculty of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606, Japan.Received for publication 6 December 1993 and in revised form 4 May 1994.1. Abbreviations used in this paper: AMI, acute myocardial infarction; ANP, atrial natriuretic peptide; BNP, brain natriuretic peptide; CHF, congestive heart failure; CNP, C-type natriuretic peptide; DOCA, deoxycorticosterone acetate; HOCM, hypertrophic obstructive cardiomyopathy; HP-GPC, high-performance gel permeation chromatography; LI, like immunoreactivity; SHR, spontaneously hypertensive rats.Since the discovery of atrial natriuretic peptide (ANP)' in the heart (1), it has been demonstrated that ANP is synthesized in and secreted from the heart, implicated in body fluid homeostasis and blood pressure control as a cardiac hormone (2). In normal hearts, ANP is produced mainly in the atrium and only slightly occurs in the ventricle, whereas in patients with congestive heart failure (CHF) and animal models of ventricular hypertrophy, ANP synthesis and secretion are greatly augmented in the ventricle (3-6).Brain natriuretic peptide (BNP), a second natriuretic peptide, was originally isolated from the porcine brain (7). This peptide shows a sequence homology to ANP (7) and has peripheral and central actions similar to t...

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Section: Discussionsupporting

confidence: 92%

“…3 B) (34,35). Moreover, a previous HPLC study showed that the major peak of ANP-LI in hamster plasma corresponds to the elution position of a-rat ANP (25).…”

Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular cloning of hamster brain and atrial natriuretic peptide cDNAs. Cardiomyopathic hamsters are useful models for brain and atrial natriuretic peptides.

Tamura¹,

Ogawa²,

Itoh³

et al. 1994

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…(32) showed that after chronic left ventricular outflow obstruction the ventricles increase their production of ANP. In the cardiomyopathic hamster, more than 70% of ANP released by the heart is produced by the ventricles (33). An increase in left subendocardial ANP content was also reported in humans with dilated cardiomyopathy and valvular heart disease (34).…”

Section: Resultsmentioning

confidence: 81%

Dietary Sodium Modulates Neonatal but Not Adult Cardiac Atrial Natriuretic Peptide in Rats

et al. 1995

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After an initial postnatal diuresis, neonates are in positive sodium balance. Because atrial natriuretic peptide (ANP) contributes to sodium homeostasis, this study was designed to evaluate the maturational effects of increased dietary sodium intake on cardiac ANP production. Preweaned Sprague-Dawley rat pups were artificially reared by feeding them either a normalsodium or high-sodium diet for 7 d and were compared with maternally reared rat pups. Adult rats were divided into three groups: the first group was given ad libitum rat food and 1% sodium chloride to drink, the second group was pair-fed with this group but given tap water to drink, and the third group was fed ad libitum rat food and water for 10 d. Atrial and ventricular pro-ANP and ANP contents and plasma ANP concentrations were measured by RIA. Steady state atrial and ventricular ANP mRNA expression was determined by Northern and dot-blot analysis. There was a Zfold increase in atrial pro-ANP and ANP content and a 50% decrease in plasma ANP concentration in preweaned rat pups fed a high-salt diet. In contrast, atrial pro-ANP and ANP content and plasma ANP concentration were not affected by increased sodium intake in adult rats. Atrial and ventricular ANP mRNA levels and ventricular pro-ANP and ANP contents were not altered by dietary sodium at either age. We conclude thal chronic increase in sodium intake in the preweaning period results in increased storage of atrial pro-ANP. The decrease in plasma ANP concentration in these preweaned rats may be due to reduced basal secretion or enhanced degradation of the peptide. (Pediutr Res 37: 310-315, 1995) Abbreviations AH, adult high-sotlium diet AL, adult ad libitum diet AN, adult normal-sodium diet ANF, atrial natriuretic factor ANP, atrial natriuretic peptide GAPDH, glyceraltlehyde 3-phosphate dehydrogenase PWH, preweaned high-sodium diet PWM, preweaned maternally reared PWN, preweaned normal-sodium diet C-receptor, clearance receptor ANP, secreted primarily from atrial myocytes, exhibits natriuretic, diuretic, and vasorelaxant properties. Since 1981, when ANP was first described by de Bold et al. (I), the production, secretion, and actions of the peptide have been further elucidated. Although atrial stretch is generally recognized as the major stimulus for ANP release, recent studies suggest an important role for sodium ingestion in ANP regulation (2-4).In term and preterm infants, there is an initial diuresis and natriuresis that occur in conjunction with an increase in plasma

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Immunocytochemical localization of atrial natriuretic factor and autoradiographic distribution of atria natriuretic factor binding sites in the brain of the African lungfish,Protopterus annectens

et al. 1996

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The localization of atrial natriuretic factor (ANF)-immunoreactive elements was investigated in the brain of the African lungfish, Protopterus annectens, by using antisera raised against rat and human ANF(1-28). Concurrently, the distribution of ANF binding sites was studied by autoradiography using radioiodinated human ANF(1-28) as a tracer. In general, there was a good correlation between the distribution of ANF-immunoreactive structures and the location of ANF binding sites in several areas of the brain, particularly in the ventral part of the medial subpallium, the rostral preoptic region, the preoptic periventricular nucleus, the caudal hypothalamus, the neural lobe of the pituitary, and the mesencephalic tectum. In contrast, mismatching was observed in the pallium (which contained a high density of binding sites and a low concentration of ANF immunoreactive elements) as well as in the lateral subpallium and the medial region of the ventral thalamus, in which a low concentration of binding sites but a high density of ANF-immunoreactive fibers were detected. The present data provide the first localization of ANF-related peptides in the brain of dipnoans and the first anatomical distribution of ANF binding sites in the brain of fish. The results show that the ANF peptidergic systems of P. annectens exhibit similarities with those previously described in the frog Rana ridibunda, supporting the existence of relationships between dipnoans and amphibians. The location of ANF-like immunoreactivity and the distribution of ANF binding sites suggest that ANF-related peptides may act as hypothalamic neurohormones as well as neurotransmitters and/or neuromodulators in the lungfish brain.

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Ventricles as a major site of atrial natriuretic factor synthesis and release in cardiomyopathic hamsters with heart failure.

Cited by 65 publications

References 67 publications

Molecular cloning of hamster brain and atrial natriuretic peptide cDNAs. Cardiomyopathic hamsters are useful models for brain and atrial natriuretic peptides.

Molecular cloning of hamster brain and atrial natriuretic peptide cDNAs. Cardiomyopathic hamsters are useful models for brain and atrial natriuretic peptides.

Dietary Sodium Modulates Neonatal but Not Adult Cardiac Atrial Natriuretic Peptide in Rats

Immunocytochemical localization of atrial natriuretic factor and autoradiographic distribution of atria natriuretic factor binding sites in the brain of the African lungfish,Protopterus annectens

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