Introduction: Ventilator Associated Pneumonia(VAP) remains a major cause of mortality and morbidity among critical ill patients. An imbalance between normal host defenses and the ability of microorganisms to colonize and invade the lower respiratory tract results in hospital acquired pneumonia. Aims and objectives were to isolate and identify bacterial pathogens from VAP and to determine their antibiogram. Material and Methods:A total of 66 Endotracheal aspirates were included in this study. 0.01 ml of sample was inoculated onto Blood agar,Chocolate agar and MacConkey agar and plates were incubated overnight at 37°C for 24 and 48 hours. Bacterial isolates were identified by standard biochemical tests. Antimicrobial sensitivity testing was performed on Muller Hinton Agar by Kirby Bauer disk diffusion and interpreted as per the Clinical and Laboratory Standards Institute (CLSI) guidelines. Results: The occurrence of VAP was more common in the age group of 18-30 years (46.51%). Of the total cases 59.09% showed monomicrobial growth and 6.06% showed polymicrobial growth. The predominant gram negative isolate obtained was Klebsiella sp (68.89%) followed by Acinetobacter sp(15.56%) and Pseudomonas aeruginosa (13.33%). Among the Gram negative bacilli, 24.44% were resistant to Piperacillin Tazobactam and 35.56% to Cefoperazone sulbactam and 31.11% isolates were resistant to meropenem.All gram negative isolates were sensitive to Imipenem. Conclusion: Knowledge on incidence of VAP, its etiology and susceptibility patterns is essential to initiate the empirical antibacterial therapy for patients on mechanical ventilation