Venoms of Neotropical wasps lack cross-reactive carbohydrate determinants enabling reliable protein-based specific IgE determination

Pérez-Riverol, Amilcar; Miehe, Michaela; Jabs, Frederic; Seismman, Henning; Fernandes, Luís Gustavo Romani; Zollner, Ricardo de Lima; Jakob, Thilo; Brochetto-Braga, Márcia Regina; Spillner, Edzard

doi:10.1016/j.jaci.2017.12.990

Cited by 18 publications

(12 citation statements)

References 11 publications

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“…Thus, CCDs do not seem to play the major role in cross-reactivity and double positive results. In addition, venoms from European and Neotropical paper wasps were shown to lack CCD, implying that diagnosis of paper wasp allergies is only hampered by peptide-based cross-reactivity [31,32]. Overall, these findings suggest that peptide based cross-reactivity plays a more important role in the differential diagnosis of HVA than previously anticipated.…”

Section: Component-resolved Diagnostics and Peptide Based Cross-reactmentioning

confidence: 76%

Current challenges in molecular diagnostics of insect venom allergy

2020

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Background Advanced component-resolved diagnostics (CRD) in Hymenoptera venom allergy (HVA) has improved the precise description of individual sensitization profiles. However, diagnostic gaps, peptidebased cross-reactivity, early identification of severe reactors and diagnosis of patients with a clear history of sting reactions but negative specific IgE and skin tests, remain challenging. Methods Systematic literature search in PubMed and critical analysis of recently published studies on insect venom allergy diagnostics. Results and discussion CRD has increased the sensitivity of IgE testing and improved the discrimination of primary sensitization from irrelevant cross-reactivity, ultimately providing a better rationale for therapeutic decisions. Despite these major advances, there is still room for improvement in routine HVA diagnostics. Peptide based cross-reactivity among homologous allergens from Vespinae and Polistinae venoms as well as still existing diagnostic gaps are particularly challenging. No marker allergens are currently available to differentiate Vespula and Polistes sensitizations. Several strategies including clinical setting of basophil activation test (BAT) for routine diagnostics, venomic analysis for the identification of novel allergens and characterization of the molecular basis of A. Perez-Riverol · T. Jakob ( )

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Section: Component-resolved Diagnostics and Peptide Based Cross-reactmentioning

confidence: 76%

Current challenges in molecular diagnostics of insect venom allergy

2020

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“…Interestingly, some serum stopped reacting to PLA1 and Ag 5 from Polybia paulista or to Ag5 from Solenopsis invicta after Brl adsorption procedures. Perez-Riverol et al [ 36 ] in recent studies have shown that PLA1 and Ag 5 allergens from Polybia paulista are glycosylated proteins and therefore do not have CCD, as in the Ag 5 from Solenopsis invicta [ 20 ]. Thus, this reduction in sera reactivity could not be related to α1,3 – fucose epitopes recognition.…”

Section: Discussionmentioning

confidence: 99%

Cross-Reactive Carbohydrate Determinant in Apis mellifera, Solenopsis invicta and Polybia paulista Venoms: Identification of Allergic Sensitization and Cross-Reactivity

Abram

Fernandes

Pérez-Riverol

et al. 2020

Toxins

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Allergic reactions to Hymenoptera venom, which could lead to systemic and even fatal symptoms, is characterized by hypersensitivity reactions mediated by specific IgE (sIgE) driven to venom allergens. Patients multisensitized to sIgE usually recognize more than one allergen in different Hymenoptera species. However, the presence of sIgE directed against Cross-Reactive Carbohydrate Determinant (CCD), which occurs in some allergens from Hymenoptera venom, hampers the identification of the culprit insects. CCD is also present in plants, pollen, fruits, but not in mammals. Bromelain (Brl) extracted from pineapples is a glycoprotein commonly used for reference to sIgE-CCD detection and analysis. In sera of fifty-one Hymenoptera allergic patients with specific IgE ≥ 1.0 KU/L, we assessed by immunoblotting the reactivity of sIgE to the major allergens of Apis mellifera, Polybia paulista and Solenopsis invicta venoms. We also distinguished, using sera adsorption procedures, the cases of CCD cross-reaction using Brl as a marker and inhibitor of CCD epitopes. The presence of reactivity for bromelain (24–28 kDa) was obtained in 43% of the patients, in which 64% presented reactivity for more than one Hymenoptera venom in radioallergosorbent (RAST) tests, and 90% showed reactivity in immunoblot analysis to the major allergens of Apis mellifera, Polybia paulista and Solenopsis invicta venoms. Sera adsorption procedures with Brl lead to a significant reduction in patients’ sera reactivity to the Hymenoptera allergens. Immunoblotting assay using pre- and post-Brl adsorption sera from wasp-allergic patients blotted with non-glycosylated recombinant antigens (rPoly p1, rPoly p5) from Polybia paulista wasp venom showed no change in reactivity pattern of sIgE that recognize allergen peptide epitopes. Our results, using Brl as a marker and CCD inhibitor to test sIgE reactivity, suggest that it could complement diagnostic methods and help to differentiate specific reactivity to allergens’ peptide epitopes from cross-reactivity caused by CCD, which is extremely useful in clinical practice.

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“…Since then, many food glycans (as xeno-glycans) were isolated, identified and structurally characterised [71,72], called cross-reactive carbohydrate determinants (CCD) [73]. CCDs generally have vast structural variability consisting of oligomannosidic hybrid or complex type structures [74,75]. These CCDs have been shown to have immunomodulatory weak allergic and/or non-allergenic immunogenic properties, either as free (unlinked) glycans or on (glyco)protein allergens [70,[76][77][78].…”

Section: Food Glycans and Antigensmentioning

confidence: 99%

Blood Type Diets (BTD) and Aging: An Overview

Menapace¹

2019

J Aging Sci

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It has recently been proposed that glycans, being the third alphabet of life, interact intricately with endogenous biomolecules to modulate tolerance, immune and inflammatory responses. Specifically, food glycans could impact health and be a source of inflammation and age-related diseases. These special carbohydrates are present as glycoconjugates (glycoproteins or glycolipids) in and on the surface of all the cells (glycocalyx) of all organisms or are found in free form in biological fluids. Recent advances in glycobiology and glycochemistry have shown how glycans bind with naturally present human proteins (lectins), through protein-carbohydrate interactions (or PCI), but also how oligosaccharides can interact with other glycans, present throughout the human body (through carbohydrate-carbohydrate interactions, or CCI). Oligosaccharides present in food sources, which go beyond the definition of normal fibers, once ingested are then either absorbed in the bloodstream, where they are recognized by the immune system, or interact with the surface of GI epithelial cells, thus generating appropriate biochemical cascades that induce a tolerance or immune/inflammatory response. Because the ABO epitopes have been encountered on all human cells, not just erythrocytes and based on the different biotypology (A, AB, B, and O) impose morphic changes in the distribution of the glycans on the glycocalyx (lipid rafts and clustered saccharide patches), their CCI with food and microbe glycans will be different, thus, eliciting contrasting responses. This can explain the epidemiological data for blood type diets (BTD). Through continuous consumption of the wrong types of glycans, processes of chronic inflammation could be initiated and progress to accelerated aging. Four basic modes of action have been identified showing how glycans can trigger inflamm-aging. Since glycobiology is a young science, further studies with newer technologies are warranted for advancement in this field.

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Venoms of Neotropical wasps lack cross-reactive carbohydrate determinants enabling reliable protein-based specific IgE determination

Cited by 18 publications

References 11 publications

Current challenges in molecular diagnostics of insect venom allergy

Current challenges in molecular diagnostics of insect venom allergy

Cross-Reactive Carbohydrate Determinant in Apis mellifera, Solenopsis invicta and Polybia paulista Venoms: Identification of Allergic Sensitization and Cross-Reactivity

Blood Type Diets (BTD) and Aging: An Overview

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