Venom alkaloids of monogyne and polygyne forms of the red imported fire ant, Solenopsis invicta, in Taiwan

Lai, Lin-Ping; Huang, Rong; Wu, Wen‐Jer

doi:10.1007/s00040-008-1025-2

Cited by 19 publications

(19 citation statements)

References 30 publications

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“…A Finnigan Focus GC was coupled to a Finnigan Focus DSQ mass selective detector (Thermo Fisher, Waltham, MA) to separate and identify the venom alkaloids of S. geminata according to our previous publication (Lai et al 2008). The mass spectral base peak at m/z ϭ 98 corresponded to the fragment C 6 H 12 N ϩ (MacConnell et al 1971).…”

Section: Collection Of Venom and Chemical Analysesmentioning

confidence: 99%

“…Our previous study showed that venom alkaloids of S. invicta vary with the social forms (monogyne versus polygyne workers) and the sampling methods (microcapillary tube versus whole body soaking) (Lai et al 2008). We hypothesized that the venom composition of major workers and minor workers of S. geminata may also differ because of different sampling methods used.…”

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confidence: 99%

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Secretion Profiles of Venom Alkaloids inSolenopsis geminata(Hymenoptera: Formicidae) in Taiwan

et al. 2009

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Solenopsis geminata (F.) was introduced into southern Taiwan decades ago and has continued to threaten the residents. Although the venom compositions of various fire ant species have been studied, the effects of environmental cues on the secretion pattern have received relatively little attention in an area with subtropical climate and high humidity, such as Taiwan. This study characterizes the effects of temperature and season on the venom compositions of S. geminata in Taiwan. Pure venom was sampled by using a microcapillary pipette and immersing the whole ant in hexane and subjected to gas chromatography-mass spectrometry analysis. The results showed that the ratio of cis C(11) to trans C(11) alkaloids in major workers was significantly higher than that in minor workers. No significant differences could be found in either the relative alkaloids content or the ratio of cis C(11) to trans C(11) alkaloids in venom of minor workers while rearing at four temperature conditions. Nevertheless, the ratio of cis C(11) to trans C(11) alkaloids in the venom of minor workers was the highest in spring and the lowest in winter. The results also showed that the body length, abdomen length, head length, head width, and venom volume differed significantly between major workers and minor workers of S. geminata. The venom volumes of these two castes were positively correlated with their body sizes.

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Section: Collection Of Venom and Chemical Analysesmentioning

confidence: 99%

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confidence: 99%

Secretion Profiles of Venom Alkaloids inSolenopsis geminata(Hymenoptera: Formicidae) in Taiwan

et al. 2009

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“…Milking venom into a capillary tube placed on the sting was first described by Blum et al (1958), and later widely used in obtaining pure venom for compositional analyses or bioassays (MacConnell et al 1970;1971;Brand et al 1972Brand et al , 1973bMacConnell et al 1976;Blum 1992;Greenberg et al 2008;Lai et al 2008Lai et al , 2009Lai et al , 2012. In this way, milligram quantities of pure venom can be obtained for direct use.…”

Section: Introductionmentioning

confidence: 99%

“…This method can be used to obtain a large amount of venom by successively extracting multiple ants. Although such extracts have been used directly in chemical analysis (Deslippe & Guo 2000;Lai et al 2008Lai et al , 2009Eliyahu et al 2011), further purification steps are needed to obtain pure venom alkaloid fractions. Chen and Fadamiro (2009a) developed a practical method to purify venom alkaloids by washing the whole body extract with a hexane-acetone solvent system containing 1 to 2% triethylamine through a silica gel column (See details in Yu et al 2014;Shi et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Lethality of Red Imported Fire Ant Venom to Argentine Ants and Other Ant Species

Greenberg¹,

Kabashima²,

Allison³

et al. 2008

Annals of the Entomological Society of America

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Using natural venom collected from the red imported fire ant, Solenopsis invicta Buren, we determined the dose of venom necessary to kill 50% of the workers (LD50) of Argentine ants, Linepithema humile (Mayr), and several other ant species that the fire ant may encounter in California. Of the species tested, the Argentine ant was most susceptible to the venom. On a weight-adjusted basis the red imported fire ant was 330 times more resistant to the venom than the Argentine ant. In addition, the native southern fire ant, Solenopsis xyloni McCook, was significantly more susceptible to the venom than S. invicta. Formica perpilosa Wheeler was most resistant to the venom when the LD50 was not adjusted for weight, requiring a dose 684 times higher than that of the Argentine ant. When we tested synthetic venom (solenopsin A, which is the main component of S. xyloni venom) against the Argentine ant, the LD50 value was unchanged. However, both S. invicta and S. xyloni were ≈6 times more susceptible to the synthetic compound than to natural venom. Therefore, S. invicta venom is less toxic to fire ants while being just as toxic to Argentine ants as the simpler, more primitive venom of S. xyloni. Fire ant gaster flagging and venom dabbing applies enough venom to the Argentine ant to be lethal and may help explain the superior interference competition shown by the red imported fire ant.

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“…Differences between the proportions of unsaturated alkaloids and ratios of C 13 : C 13:1 and C 15 :C 15:1 alkaloids in the venom of the monogyne and polygyne forms of S. invicta have been demonstrated (Lai et al 2008). The effects of environmental cues on the venom compositions of S. invicta and S. geminata in Taiwan were further characterized in our previous studies (Lai et al 2008(Lai et al , 2009).…”

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confidence: 89%

The Insecticidal Activities of Fire Ant (Hymenoptera: Formicidae) Venoms Against <I>Plutella xylostella</I> (Lepidoptera: Plutellidae) Larvae

et al. 2012

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Although fire ants frequently have negative impacts on agricultural systems and public health, they have additional beneficial insecticidal effects. To evaluate the potential effect of fire ant venoms on agricultural pests, the compositions of the venoms and their insecticidal activities against Plutella xylostella (L.) larvae were evaluated under laboratory conditions. The alkaloids found in Solenopsis geminata (F.) venom are primarily saturated C11, which occur in both cis and trans forms, whereas the venom of S. invicta Buren contains six principal alkaloids (from trans C1, to C17). Moreover, the proportions of unsaturated alkaloids in the venom of polygynous S. invicta were significantly higher than the corresponding proportions in the monogynous S. invicta, as shown by our previous studies. Fire ant venoms were topically applied to the dorsal thoracic region of fourth-instar larvae of P. xylostella. The results of the experiment showed that the larval symptoms induced by fire ant venom include contractile, flaccid paralysis, black coloration and death. P. xylostella larvae were most susceptible to S. geminata venom. The order of the susceptibilities of the larvae to the venoms was as follows: S. geminata > S. invicta (monogyne form) > S. invicta (polygyne form), as measured by the corresponding LT50 values at 24 h.

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Venom alkaloids of monogyne and polygyne forms of the red imported fire ant, Solenopsis invicta, in Taiwan

Cited by 19 publications

References 30 publications

Secretion Profiles of Venom Alkaloids inSolenopsis geminata(Hymenoptera: Formicidae) in Taiwan

Secretion Profiles of Venom Alkaloids inSolenopsis geminata(Hymenoptera: Formicidae) in Taiwan

Lethality of Red Imported Fire Ant Venom to Argentine Ants and Other Ant Species

The Insecticidal Activities of Fire Ant (Hymenoptera: Formicidae) Venoms Against <I>Plutella xylostella</I> (Lepidoptera: Plutellidae) Larvae

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