Venezuelan Equine Encephalitis Complex Alphavirus in Bats, French Guiana

Fischer, Carlo; Pontier, Dominique; Filippi‐Codaccioni, Ondine; Pons, Jean-Batiste; Postigo-Hidalgo, Ignacio; Duhayer, Jeanne; Drexler, Jan Felix

doi:10.3201/eid2704.202676

Cited by 11 publications

(8 citation statements)

References 14 publications

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“…In Colombia, VEEV viral RNA and antigen detection were demonstrated via RT-PCR and immunohistochemistry (IHC) in the brain tissues from two frugivorous bats, suggesting that these species might be hosts for this zoonotic agent [46]. Finally, a new strain of encephalitogenic VEEV (Tonate Virus) was isolated from bats in French Guiana [47]. Neutralizing antibodies against SLEV have been reported in bats from Ohio, USA [48].…”

Section: Discussionmentioning

confidence: 99%

Serological Positivity against Selected Flaviviruses and Alphaviruses in Free-Ranging Bats and Birds from Costa Rica Evidence Exposure to Arboviruses Seldom Reported Locally in Humans

Murillo

Piche-Ovares

Gamboa-Solano

et al. 2022

Viruses

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Arboviruses have two ecological transmission cycles: sylvatic and urban. For some, the sylvatic cycle has not been thoroughly described in America. To study the role of wildlife in a putative sylvatic cycle, we sampled free-ranging bats and birds in two arbovirus endemic locations and analyzed them using molecular, serological, and histological methods. No current infection was detected, and no significant arbovirus-associated histological changes were observed. Neutralizing antibodies were detected against selected arboviruses. In bats, positivity in 34.95% for DENV-1, 16.26% for DENV-2, 5.69% for DENV-3, 4.87% for DENV-4, 2.43% for WNV, 4.87% for SLEV, 0.81% for YFV, 7.31% for EEEV, and 0.81% for VEEV was found. Antibodies against ZIKV were not detected. In birds, PRNT results were positive against WNV in 0.80%, SLEV in 5.64%, EEEV in 8.4%, and VEEV in 5.63%. An additional retrospective PRNT analysis was performed using bat samples from three additional DENV endemic sites resulting in a 3.27% prevalence for WNV and 1.63% for SLEV. Interestingly, one sample resulted unequivocally WNV positive confirmed by serum titration. These results suggest that free-ranging bats and birds are exposed to not currently reported hyperendemic-human infecting Flavivirus and Alphavirus; however, their role as reservoirs or hosts is still undetermined.

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Section: Discussionmentioning

confidence: 99%

Serological Positivity against Selected Flaviviruses and Alphaviruses in Free-Ranging Bats and Birds from Costa Rica Evidence Exposure to Arboviruses Seldom Reported Locally in Humans

Murillo

Piche-Ovares

Gamboa-Solano

et al. 2022

Viruses

View full text Add to dashboard Cite

show abstract

“…In Colombia, VEEV viral RNA and antigen detection were demonstrated via RT-PCR and immunohistochemistry (IHC)in the brain tissues from two frugivorous bats, suggesting that these species might be hosts for this zoonotic agent [38]. Finally, a new strain of encephalitogenic VEEV (Tonate Virus) was isolated from bats in French Guiana [39]. Neutralizing antibodies against SLEV have been reported in bats from Ohio, USA?…”

Section: Discussionmentioning

confidence: 99%

Serological Positivity against Selected <em>Flavivirus </em>and <em>Alphavirus</em> in Free-ranging Bats and Birds from Costa Rica Evidence Exposure to Arboviruses Seldom Reported Locally in Humans

Murillo¹,

Piche-Ovares²,

Solano³

et al. 2021

Preprint

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Arboviruses have two ecological transmission cycles, sylvatic and urban. For some, the sylvatic cycle has not been thoroughly described in America. To study the role of wildlife in a putative sylvatic cycle, we sampled free-ranging bats and birds in two arbovirus endemic locations and analyzed them using molecular, serological, and histological methods. No current infection was detected, and no significant arbovirus-associated histological changes were observed. Neutralizing antibodies were detected against selected arboviruses. In bats, positivity in 34.95% for DENV-1, 16.26% for DENV-2, 5.69% for DENV-3, 4.87% for DENV-4, 2.43% for WNV, 4.87% for SLEV, 0,81% for YFV, 7.31% for EEEV, and 0.81% for VEEV was found. Antibodies against ZIKV were not detected. In birds, PRNT results were positive against WNV in 0.80%, SLEV in 5.64%, EEEV in 8.4%, and VEEV in 5.63%. An additional retrospective PRNT analysis was performed using bat samples from three additional DENV endemic sites resulting in a 3.27% prevalence for WNV and 1.63% for SLEV. Interestingly one sample resulted unequivocally WNV positive confirmed by serum titration. These results suggest that free-ranging bats and birds are exposed to not currently reported hyperendemic-human infecting Flavivirus and Alphavirus, however, their role as reservoirs or hosts is still undetermined.

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“…(Mel.) spissipes Theobald are highly suspected to be natural vectors of Tonate virus (TONV) that belongs to the Alphavirus genus in FG [25][26][27], but are also found infected by Bunyavirus (Caru, group Guama, Caraparu and group C) [19]. Culex (Mel.)…”

Section: Of 18mentioning

confidence: 99%

Identification of Neotropical Culex Mosquitoes by MALDI-TOF MS Profiling

Costa¹,

Guidez²,

Briolant³

et al. 2023

Preprint

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French Guiana mosquito fauna diversity encompasses 242 species, of which nearly half of them belong to Culex (Diptera: Culicidae) genus. Whereas several species from Culex genus are important vectors of arbovirus, scarce studies focus on Culex genus, notably due to the inaccurate morphological identification of field-caught females gender. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been reported as a promising method for the identification of mosquitoes. Culex females from French Guiana field were dissected based on morphological classification. Abdomens were used for molecular identification using COI (cytochrome oxydase 1) gene. Legs and thorax of 169 specimens belonging to 13 Culex species,( i.e. Cx. declarator, Cx. nigripalpus, Cx. quinquefasciatus, Cx. usquatus, Cx. adamesi, Cx. dunni, Cx. eastor, Cx. idottus, Cx. pedroi, Cx. phlogistus, Cx. portesi, Cx. rabanicolus and Cx. spissipes) were then submitted to MALDI-TOF MS analysis. A high intra-species reproducibility and inter-species specificity of MS spectra for each mosquito body part tested were noticed. A corroboration of the specimen identification was revealed between MALDI-TOF MS, morphological and molecular results. MALDI-TOF MS protein profiling proves to be a suitable tool for identification of neotropical Culex species and will permit to enhance knowledge on this highly diverse Culex mosquitoes genus.

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Venezuelan Equine Encephalitis Complex Alphavirus in Bats, French Guiana

Cited by 11 publications

References 14 publications

Serological Positivity against Selected Flaviviruses and Alphaviruses in Free-Ranging Bats and Birds from Costa Rica Evidence Exposure to Arboviruses Seldom Reported Locally in Humans

Serological Positivity against Selected Flaviviruses and Alphaviruses in Free-Ranging Bats and Birds from Costa Rica Evidence Exposure to Arboviruses Seldom Reported Locally in Humans

Serological Positivity against Selected <em>Flavivirus </em>and <em>Alphavirus</em> in Free-ranging Bats and Birds from Costa Rica Evidence Exposure to Arboviruses Seldom Reported Locally in Humans

Identification of Neotropical Culex Mosquitoes by MALDI-TOF MS Profiling

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