Velvet Antler Ameliorates Cardiac Function by Restoring Sarcoplasmic Reticulum Ca2+-ATPase Activity in Rats With Heart Failure After Myocardial Infarction

Abstract

Objective: Velvet antler (VA; cornu cervi pantotrichum), a well-known traditional Chinese medicine, has been shown to exert cardioprotective effects. The purpose of this study was to investigate the effect of VA on heart failure (HF) caused by ischemia-reperfusion, and explore its possible mechanism from the regulation of sarcoplasmic/endoplasmic reticulum Ca2+-ATPase 2 alpha (SERCA2a).Methods: A rat model of HF was established by ligating the left anterior descending coronary artery of male Sprague–Dawley rats (n = 88). One week after surgery, VA (200, 400, or 800 mg/[kg day−1]) or enalapril (1 mg/[kg day−1]) was administered daily for the next 4 weeks. Heart function was detected by echocardiography and histopathological analysis. The serum BNP level was measured by ELISA, and the expression of SERCA2a, PLB, PLB-Ser16, and PKA was determined by western blotting. SERCA2a and PLB mRNA levels were determined by real-time quantitative PCR.Results: Compared with the sham group, cardiac function in the HF group, including the serum BNP level, heart mass index, myocardial collagen deposition, and left ventricular ejection fraction, was markedly reduced; however, these changes could be reversed by VA treatment. In addition, VA (200 mg/[kg·d−1]) inhibited the decrease of SERCA2a and PLB mRNA levels and SERCA2a, PLB, PLB-Ser16, and PKA protein expression and restored the activity of SERCA2a and PKA. Enalapril affected only PLB protein expression.Conclusion: VA can improve myocardial fibrosis and ventricular remodeling in rats, thereby helping to restore cardiac function. The underlying mechanism may be related to the upregulation of the expression and activation of PKA and PLB and the restoration of the expression and activity of SERCA2a.

Jintiange Capsule Alleviates Rheumatoid Arthritis and Reverses Changes of Serum Metabolic Profile in Collagen-Induced Arthritic Rats

“…LC–MS analysis and data mining were processed according to the methods described in the literature. 35 , 36 …”

“…LC-MS analysis and data mining were processed according to the methods described in the literature. 35,36 Animal Treatments Sixty 7-week-old male Wistar rats weighing (170 ± 10) g (Sippur Will Kay Company) were housed at the Experimental Animal Center of Zhejiang Chinese Medical University (Hangzhou, China, Certificate No. SCXK 2018-0012) and acclimatized for a week under the standard conditions at (24 ± 0.5) °C with 12-hour light-dark cycles, fed standard rodent chow with free access to water.…”

Inhibition of Myocardial Cell Apoptosis Is Important Mechanism for Ginsenoside in the Limitation of Myocardial Ischemia/Reperfusion Injury

“…Mitochondria play a significant role in adjusting metabolism, generating ROS, and guaranteeing cell activity (Zhang et al, 2019a;Zhang X. et al, 2021). Bcl-2 inhibits apoptosis, whereas Bax promotes it, causing damage to the membrane structure and potential of mitochondria (Wang Q. et al, 2016).…”

“…And NF-κB has function of inhibiting anti-apoptotic protein Bcl-2 ( Neamatallah et al, 2018 ). MIRI up-regulates the levels of reactive oxygen species (ROS) and ER stress and further leads to intracellular calcium overload and apoptosis ( Chaudhari et al, 2014 ; Lai et al, 2020 ; Song et al, 2020 ; Shi et al, 2021 ). ROS activates ER stress and JNK to increase the content of ROS ( Chaudhari et al, 2014 ; Son et al, 2014 ; Lu et al, 2017 ; Chu et al, 2019 ).…”

Velvet antler water extract protects porcine oocytes from lipopolysaccharide‐induced meiotic defects

“…Previous reports have indicated that VA had anti‐inflammatory effects on human rheumatoid arthritis fibroblast‐like synoviocytes and alleviated the Parkinson's disease by inhibiting oxidative stress in mice, 17,18 further demonstrating that VA had many beneficial components and pharmacological actions 19–22 . Also, other studies have shown that VA could rescue the sarcoplasmic reticulum Ca 2+ ‐ATPase activity and thus enhanced the cardiac function in rats with heart failure 23,24 . However, whether VA could improve the quality of oocytes under oxidative stress from diverse environmental factors is largely elusive.…”