Vehicles and ways for efficient nuclear transformation in plants

Husaini, Amjad M.; Abdin, Malik Zainul; Parray, G. A.; Sanghera, Gulzar S.; Murtaza, Imtiyaz; Alam, Tanweer; Srivastava, D. K.; Farooqi, Humaira; Khan, Hamzullah

doi:10.4161/gmcr.1.5.14660

Cited by 23 publications

(9 citation statements)

References 176 publications

(184 reference statements)

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“…Tissue culture is an essential technique of plant propagation and development, and has been used to generate doubled haploids (Morrison and Evans 1988) and transformed plants (Husaini et al 2010), for conservation (Li and Pritchard 2009) and to produce new varieties (Patial et al 2019). Protocols have been developed for the regeneration of crop and model plant species that speed up the time of obtaining plants (Patial et al 2019), eliminate disease (García-Gonzáles et al 2010) and continually produce hundreds of thousands of regenerants.…”

Section: Discussionmentioning

confidence: 99%

Precise evaluation of tissue culture-induced variation during optimisation of in vitro regeneration regime in barley

Orłowska

Bednarek

2020

Plant Mol Biol

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Key message The Taguchi method and metAFLP analysis were used to optimise barley regenerants towards maximum andminimum levels of tissue culture-induced variation. The subtle effects of symmetric and asymmetric methylationchanges in regenerants were identified. Abstract Plant tissue cultures (PTCs) provide researchers with unique materials that accelerate the development of new breeding cultivars and facilitate studies on off-type regenerants. The emerging variability of regenerants derived from PTCs may have both genetic and epigenetic origins, and may be desirable or degrade the value of regenerated plants. Thus, it is crucial to determine how the PTC variation level can be controlled. The easiest way to manipulate total tissue cultureinduced variation (TTCIV) is to utilise appropriate stress factors and suitable medium components. This study describes the optimisation of in vitro tissue culture-induced variation in plant regenerants derived from barley anther culture, and maximizes and minimizes regenerant variation compared with the source explants. The approach relied on methylation amplified fragment length polymorphism (metAFLP)-derived TTCIV characteristics, which were evaluated in regenerants derived under distinct tissue culture conditions and analysed via Taguchi statistics. The factors that may trigger TTCIV included CuSO 4 , AgNO 3 and the total time spent on the induction medium. The donor plants prepared for regeneration purposes had 5.75% and 2.01% polymorphic metAFLP loci with methylation and sequence changes, respectively. The level of TTCIV (as the sum of all metAFLP characteristics analyzed) identified in optimisation and verification experiments reached 7.51 and 10.46%, respectively. In the trial designed to produce a minimum number of differences between donor and regenerant plants, CuSO 4 and AgNO 3 were more crucial than time, which was not a significant factor. In the trial designed to produce a maximum number of differences between donor and regenerant plants, all factors had comparable impact on variation. The Taguchi method reduced the time required for experimental trials compared with a grid method and suggested that medium modifications were required to control regenerant variation. Finally, the effects of symmetric and asymmetric methylation changes on regenerants were identified using novel aspects of the metAFLP method developed for this analysis.

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Section: Discussionmentioning

confidence: 99%

Precise evaluation of tissue culture-induced variation during optimisation of in vitro regeneration regime in barley

Orłowska

Bednarek

2020

Plant Mol Biol

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“…Existing nanoparticle‐based methods still rely heavily on tools like the gene gun, where DNA‐coated gold microparticles are used as bullets for bombardment of plant cells and tissues to achieve gene transfer . These methods require specialized, expensive equipment and lead to significant plant cell damage due to high delivery pressures . To address these limitations, there is an urgent need to understand how nanoparticles interact with various biological membranes within plant cells.…”

Section: Introductionmentioning

confidence: 99%

Rational Design Principles for the Transport and Subcellular Distribution of Nanomaterials into Plant Protoplasts

Lew

Wong

Kwak

et al. 2018

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111

139

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The ability to control the subcellular localization of nanoparticles within living plants offers unique advantages for targeted biomolecule delivery and enables important applications in plant bioengineering. However, the mechanism of nanoparticle transport past plant biological membranes is poorly understood. Here, a mechanistic study of nanoparticle cellular uptake into plant protoplasts is presented. An experimentally validated mathematical model of lipid exchange envelope penetration mechanism for protoplasts, which predicts that the subcellular distribution of nanoparticles in plant cells is dictated by the particle size and the magnitude of the zeta potential, is advanced. The mechanism is completely generic, describing nanoparticles ranging from quantum dots, gold and silica nanoparticles, nanoceria, and single-walled carbon nanotubes (SWNTs). In addition, the use of imaging flow cytometry to investigate the influence of protoplasts' morphological characteristics on nanoparticle uptake efficiency is demonstrated. Using DNA-wrapped SWNTs as model nanoparticles, it is found that glycerolipids, the predominant lipids in chloroplast membranes, exhibit stronger lipid-nanoparticle interaction than phospholipids, the major constituent in protoplast membrane. This work can guide the rational design of nanoparticles for targeted delivery into specific compartments within plant cells without the use of chemical or mechanical aid, potentially enabling various plant engineering applications.

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“…Pressure elevation or suction was found to mediate the insertion of naked DNA and oligonucleotide into neointimal, medial and adventitial cells of rabbit carotid arteries 10 , rat and human cardiovascular tissues 11 , and naked plasmid DNA and siRNAs into mice tissue cells 12 , but like biochemical means, such methods are not effective for suspended cell types for which pressure changes are not easy to be induced. Electroporation has also been used to deliver genetic materials into certain cell types which are hard to be transfected otherwise 6 13 14 , but the method normally jeopardizes viability and possibly results in ion imbalance that may lead to improper cell functions 15 .…”

mentioning

confidence: 99%

Mechanical oscillations enhance gene delivery into suspended cells

Zhou

Sun

et al. 2016

Sci Rep

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Suspended cells are difficult to be transfected by common biochemical methods which require cell attachment to a substrate. Mechanical oscillations of suspended cells at certain frequencies are found to result in significant increase in membrane permeability and potency for delivery of nano-particles and genetic materials into the cells. Nanomaterials including siRNAs are found to penetrate into suspended cells after subjecting to short-time mechanical oscillations, which would otherwise not affect the viability of the cells. Theoretical analysis indicates significant deformation of the actin-filament network in the cytoskeleton cortex during mechanical oscillations at the experimental frequency, which is likely to rupture the soft phospholipid bilayer leading to increased membrane permeability. The results here indicate a new method for enhancing cell transfection.

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Vehicles and ways for efficient nuclear transformation in plants

Cited by 23 publications

References 176 publications

Precise evaluation of tissue culture-induced variation during optimisation of in vitro regeneration regime in barley

Precise evaluation of tissue culture-induced variation during optimisation of in vitro regeneration regime in barley

Rational Design Principles for the Transport and Subcellular Distribution of Nanomaterials into Plant Protoplasts

Mechanical oscillations enhance gene delivery into suspended cells

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