VEGFA-modified DPSCs combined with LC-YE-PLGA NGCs promote facial nerve injury repair in rats

Xu, Wanqiu; Xu, Xiaohang; Yao, Lihong; Xue, Bing; Xi, Hualei; Cao, Xi‐Ren; Piao, Guiyan; Song, Lin; Wang, Xiumei

doi:10.1016/j.heliyon.2023.e14626

Cited by 9 publications

(4 citation statements)

References 46 publications

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“…A study by Xu et al (2023) investigated the effects of a nerve conduit made of PLGA, containing schwann cells and chitosan, on nerve regeneration in rats. The researchers found that the combination of the conduit, schwann cells, and chitosan led to significantly improved nerve regeneration compared to the control group, with increased functional recovery [41].…”

Section: Discussionmentioning

confidence: 99%

Enhancement of nerve regeneration through schwann cell-mediated healing in a 3D printed polyacrylonitrile conduit incorporating hydrogel and graphene quantum dots: a study on rat sciatic nerve injury model

Hoveizi

2023

Biomed. Mater.

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Despite recent technological advancements, effective healing from sciatic nerve damage remains inadequate. Cell-based therapies offer a promising alternative to autograft restoration for peripheral nerve injuries, and 3D printing techniques can be used to manufacture conduits with controlled diameter and size. In this study, we investigated the potential of Wharton’s jelly-derived mesenchymal stem cells (WJMSCs) differentiated into schwann cells, using a polyacrylonitrile (PAN) conduit filled with fibrin hydrogel and graphene quantum dots (GQDs) to promote nerve regeneration in a rat sciatic nerve injury model. We investigated the potential of WJMSCs, extracted from the umbilical cord, to differentiate into schwann cells and promote nerve regeneration in a rat sciatic nerve injury model. WJMSCs were 3D cultured and differentiated into schwann cells within fibrin gel for two weeks. A 3 mm defect was created in the sciatic nerve of the rat model, which was then regenerated using a conduit/fibrin, conduit covered with schwann cells in fibrin/GQDs, GQDs in fibrin, and a control group without any treatment (n = 6/group). At 10 weeks after transplantation, motor and sensory functions and histological improvement were assessed. The WJMSCs were extracted, identified, and differentiated. The differentiated cells expressed typical schwann cell markers, S100 and P75. In vivo investigations established the durability and efficacy of the conduit to resist the pressures over two months of implantation. Histological measurements showed conduit efficiency, schwann cell infiltration, and association within the fibrin gel and lumen. Rats treated with the composite hydrogel-filled PAN conduit with GQDs showed significantly higher sensorial recovery than the other groups. Histological results showed that this group had significantly more axon numbers and remyelination than others. Our findings suggest that the conduit/schwann approach has the potential to improve nerve regeneration in peripheral nerve injuries, with future therapeutic implications.

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Section: Discussionmentioning

confidence: 99%

Enhancement of nerve regeneration through schwann cell-mediated healing in a 3D printed polyacrylonitrile conduit incorporating hydrogel and graphene quantum dots: a study on rat sciatic nerve injury model

Hoveizi

2023

Biomed. Mater.

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“…Various studies highlight DPSCs’ potential for neurodegeneration. Recent research demonstrates that combining vascular endothelial growth factor A (VEGFA)-overexpressing rat dental pulp stem cells (rDPSCs) with a laminin-coated and yarn-encapsulated poly (l-lactide-co-glycolide) (PLGA) nerve guidance conduit (LC-YE-PLGA NGC) enhances myelin sheath quantity, thickness, and axonal diameter, facilitating the repair of facial nerve injuries [ 53 ]. Similar experiments show that chitosan tubes inoculated with stem cell factor and DPSCs boost neo-vascularization, providing an effective approach to repairing facial nerve defects [ 54 ].…”

Section: The Neurodegenerative Potential Of Dental Stem Cellsmentioning

confidence: 99%

Advancements in Spinal Cord Injury Repair: Insights from Dental-Derived Stem Cells

Wen,

Jiang,

et al. 2024

Biomedicines

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Spinal cord injury (SCI), a prevalent and disabling neurological condition, prompts a growing interest in stem cell therapy as a promising avenue for treatment. Dental-derived stem cells, including dental pulp stem cells (DPSCs), stem cells from human exfoliated deciduous teeth (SHED), stem cells from the apical papilla (SCAP), dental follicle stem cells (DFSCs), are of interest due to their accessibility, minimally invasive extraction, and robust differentiating capabilities. Research indicates their potential to differentiate into neural cells and promote SCI repair in animal models at both tissue and functional levels. This review explores the potential applications of dental-derived stem cells in SCI neural repair, covering stem cell transplantation, conditioned culture medium injection, bioengineered delivery systems, exosomes, extracellular vesicle treatments, and combined therapies. Assessing the clinical effectiveness of dental-derived stem cells in the treatment of SCI, further research is necessary. This includes investigating potential biological mechanisms and conducting Large-animal studies and clinical trials. It is also important to undertake more comprehensive comparisons, optimize the selection of dental-derived stem cell types, and implement a functionalized delivery system. These efforts will enhance the therapeutic potential of dental-derived stem cells for repairing SCI.

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“…With strong mechanical properties and the ability to efficiently penetrate nutrients, this conduit offers great potential for use in maintaining a stable support struc-ture for nerve regeneration [25]. Xu et al produced dental pulp stem cells overexpressing VEGFA in conjunction with PLGA conduits via electrospinning to heal 10 mm facial nerve defects in rats [86]. The composite conduit availed the growth of axons and myelin sheath (Figure 8a-c), which can hasten the healing process following facial nerve damage and enhance the degree of motor ability recovery.…”

Section: Plgamentioning

confidence: 99%

Porous Organic Materials in Tissue Engineering: Recent Advances and Applications for Severed Facial Nerve Injury Repair

Sun,

Cao,

Pan

et al. 2024

Molecules

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The prevalence of facial nerve injury is substantial, and the restoration of its structure and function remains a significant challenge. Autologous nerve transplantation is a common treatment for severed facial nerve injury; however, it has great limitations. Therefore, there is an urgent need for clinical repair methods that can rival it. Tissue engineering nerve conduits are usually composed of scaffolds, cells and neurofactors. Tissue engineering is regarded as a promising method for facial nerve regeneration. Among different factors, the porous nerve conduit made of organic materials, which has high porosity and biocompatibility, plays an indispensable role. This review introduces facial nerve injury and the existing treatment methods and discusses the necessity of the application of porous nerve conduit. We focus on the application of porous organic polymer materials from production technology and material classification and summarize the necessity and research progress of these in repairing severed facial nerve injury, which is relatively rare in the existing articles. This review provides a theoretical basis for further research into and clinical interventions on facial nerve injury and has certain guiding significance for the development of new materials.

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VEGFA-modified DPSCs combined with LC-YE-PLGA NGCs promote facial nerve injury repair in rats

Cited by 9 publications

References 46 publications

Enhancement of nerve regeneration through schwann cell-mediated healing in a 3D printed polyacrylonitrile conduit incorporating hydrogel and graphene quantum dots: a study on rat sciatic nerve injury model

Enhancement of nerve regeneration through schwann cell-mediated healing in a 3D printed polyacrylonitrile conduit incorporating hydrogel and graphene quantum dots: a study on rat sciatic nerve injury model

Advancements in Spinal Cord Injury Repair: Insights from Dental-Derived Stem Cells

Porous Organic Materials in Tissue Engineering: Recent Advances and Applications for Severed Facial Nerve Injury Repair

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