VEGF165 and bFGF protein based therapy in a slow release system to improve angiogenesis in a bioartificial dermal substitute in vitro and in vivo

Transplantation of artificial gelatin-co-bletillastriata gelatin/<i>Salvia miltiorrhiza</i> Corium promotes dermal repair in rats

“…Meanwhile, Wilcke et al found that incorporation of VEGF and β-FGF into fibrin matrix materials could result in the construction of dermal substitutes, which could accelerate the formation of new blood vessels and improve graft survival when replanted into the full-thickness skin wounds of nude mice [14]. Furthermore, Pienimaki et al [15] found that EGF could significantly accelerate wound healing and promote keratinocytes to produce hyaluronan and Cuono et al [16] constructed a deepidermalized dermis (DED) with cadaver skin, which retained the scaffold and full basement membrane complex without immunogenicity, and the epidermal cells could differentiate into a multilayered epidermis on the DED.…”

Carbon nanotubes as VEGF carriers to improve the early vascularization of porcine small intestinal submucosa in abdominal wall defect repair

“…However, encapsulated VEGF is often uncontrollably released from the carrier matrix. 12,13 Using fibrin matrices, Wilcke et al 14 developed a delayed-release system for the delivery of VEGF 165 and basic fibroblast growth factor to repair full-skin defects on the backs of mice. After the first 48 hours, the concentration of VEGF 165 released decreased by 50% compared to that in the first 24 hours.…”

Angiogenesis and Vascularity for Tissue Engineering Applications

“…As previously discussed, a successful angiogenic outcome is not solely attributed to VEGF implementation alone (discussed in Section 3 and Table 1). Wilcke, et al, showcased the coupling of VEGF with bFGF in a fibrin dermal substitute (Wilcke et al, 2007). This led to a marked improvement in factor delivery, with a notable, prolonged release and resulted in a higher density of newly developed vessels in in vivo murine models.…”