VEGF Gene Therapy Augments Localized Angiogenesis and Promotes Anastomotic Wound Healing: A Pilot Study in a Clinically Relevant Animal Model

Enestvedt, C. Kristian; Hosack, Luke; Winn, Shelley R.; Diggs, Brian S.; Uchida, Barry T.; O’Rourke, Robert W.; Jobe, Blair A.

doi:10.1007/s11605-008-0635-3

Cited by 27 publications

(16 citation statements)

References 34 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The potential importance of angiogenesis has been further highlighted by a study reporting the acceleration of wound healing by vascular endothelial cell growth factor (VEGF) in a rabbit model of colonic anastomoses, 57 and improved anastomotic healing in an opossum esophagectomy model by using gene therapy using VEGF transfection 58 showing a strong correlation between bursting pressure and the amount of neovascularization. Glucocorticoids could influence angiogenesis through several mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Effect of Preoperative Single-Dose Corticosteroid Administration on Postoperative Morbidity Following Esophagectomy

Engelman

Maeyens

2010

Journal of Gastrointestinal Surgery

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of Preoperative Single-Dose Corticosteroid Administration on Postoperative Morbidity Following Esophagectomy

Engelman

Maeyens

2010

Journal of Gastrointestinal Surgery

View full text Add to dashboard Cite

show abstract

“…Anastomotic leak related to ischemia is a source of significant morbidity and mortality in gastrointestinal surgery. 3 Experimental data unequivocally show persisting ischemia to impair the development of anastomotic strength. insult.…”

Section: Introductionmentioning

confidence: 99%

Early Anastomotic Repair in the Rat Intestine is Affected by Transient Preoperative Mesenteric Ischemia

Posma

Bleichrodt

Lomme

et al. 2009

Journal of Gastrointestinal Surgery

View full text Add to dashboard Cite

Introduction During bowel surgery, perioperative blood loss and hypotension can lead to transient intestinal ischemia. Recent preclinical studies reveal that the strength of intestinal anastomoses can be compromised after reperfusion. So far, this phenomenon has not been investigated in the very first days of healing when wound strength is lowest. Material and Method Ischemia was induced in rats by clamping both the superior mesenteric artery and ileal branches for 30 min. Immediately after declamping, anastomoses were constructed in both terminal ileum and descending colon. The same was done in control groups after sham-ischemia. Anastomotic bursting pressure and breaking strength were measured immediately after operation (day 0) and after 1, 2, or 3 days. Anastomotic hydroxyproline content, gelatinase activity, and histology were analyzed. Results and Discussion In ileal anastomoses, at day 1, both the breaking strength and bursting pressure were significantly (p<0.05) lower in the ischemic group, while at day 2, this was the case for the bursting pressure only. In the colon, the bursting pressure in the ischemic group was lower at day 1. Anastomotic hydroxyproline content remained unchanged. Increased presence of the various gelatinase activities was found in ileum only at day 0 and in colon at days 1 and 2. Histological mucosal damage was found in ischemia-reperfusion groups. Conclusion Transient mesenteric ischemia can negatively affect anastomotic strength during the very first days of healing, even if the tissue used for anastomotic construction looks vital.

show abstract

“…The Laser Doppler perfusion image index (LDPII) is a standard tool in the determination of perfusion and microcirculation in the investigation of different diseases correlating to tissue ischemia [24, 25]. It directly correlates with arteriogenesis [26].…”

Section: Discussionmentioning

confidence: 99%

Effect of Extracorporeal Shock Wave Treatment on Deep Partial-Thickness Burn Injury in Rats: A Pilot Study

Djedovic

Kamelger

Jeschke

et al. 2014

Plastic Surgery International

View full text Add to dashboard Cite

Extracorporeal shock wave therapy (ESWT) enhances tissue vascularization and neoangiogenesis. Recent animal studies showed improved soft tissue regeneration using ESWT. In most cases, deep partial-thickness burns require skin grafting; the outcome is often unsatisfactory in function and aesthetic appearance. The aim of this study was to demonstrate the effect of ESWT on skin regeneration after deep partial-thickness burns. Under general anesthesia, two standardized deep partial-thickness burns were induced on the back of 30 male Wistar rats. Immediately after the burn, ESWT was given to rats of group 1 (N = 15), but not to group 2 (N = 15). On days 5, 10, and 15, five rats of each group were analyzed. Reepithelialization rate was defined, perfusion units were measured, and histological analysis was performed. Digital photography was used for visual documentation. A wound score system was used. ESWT enhanced the percentage of wound closure in group 1 as compared to group 2 (P < 0.05). The reepithelialization rate was improved significantly on day 15 (P < 0.05). The wound score showed a significant increase in the ESWT group. ESWT improves skin regeneration of deep partial-thickness burns in rats. It may be a suitable and cost effective treatment alternative in this type of burn wounds in the future.

show abstract

VEGF Gene Therapy Augments Localized Angiogenesis and Promotes Anastomotic Wound Healing: A Pilot Study in a Clinically Relevant Animal Model

Abstract: This description of gene therapy in gastrointestinal surgery using VEGF(165) transfection demonstrates increased angiogenesis with subsequently improved anastomotic healing in a clinically relevant model.

Cited by 27 publications

References 34 publications

Effect of Preoperative Single-Dose Corticosteroid Administration on Postoperative Morbidity Following Esophagectomy

Effect of Preoperative Single-Dose Corticosteroid Administration on Postoperative Morbidity Following Esophagectomy

Early Anastomotic Repair in the Rat Intestine is Affected by Transient Preoperative Mesenteric Ischemia

Effect of Extracorporeal Shock Wave Treatment on Deep Partial-Thickness Burn Injury in Rats: A Pilot Study

Contact Info

Product

Resources

About