Vegetative propagation of<i>Ceanothus velutinus</i>using stem cuttings

Paudel, Asmita; Sun, Youping; Rupp, Larry; Carman, John G.; Love, Stephen L.

doi:10.3368/npj.23.1.123

Cited by 2 publications

(3 citation statements)

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“…The wounded stems were dipped in 3,000 mg/L indole butyric acid (a rooting hormone Hormodin 2). For the control set, the treated cuttings were placed in peat moss and perlite (1:4 v/v) soil mixture ( Paudel et al., 2022 ). In the second set, 50% of the soil mix was replaced with bulk soil from the snowbrush ceanothus plants in Tony Grove in Logan Canyon to make it 50% of native soil inoculum to observe the effect of microbiome in the native soil on the rooting.…”

Section: Methodsmentioning

confidence: 99%

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IAA-producing plant growth promoting rhizobacteria from Ceanothus velutinus enhance cutting propagation efficiency and Arabidopsis biomass

Ganesh,

Hewitt,

Devkota

et al. 2024

Front. Plant Sci.

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Climate-induced drought impacts plant growth and development. Recurring droughts increase the demand for water for food production and landscaping. Native plants in the Intermountain West region of the US are of keen interest in low water use landscaping as they are acclimatized to dry and cold environments. These native plants do very well at their native locations but are difficult to propagate in landscape. One of the possible reasons is the lack of associated microbiome in the landscaping. Microbiome in the soil contributes to soil health and impacts plant growth and development. Here, we used the bulk soil from the native plant Ceanothus velutinus (snowbrush ceanothus) as inoculant to enhance its propagation. Snowbrush ceanothus is an ornamental plant for low-water landscaping that is hard to propagate asexually. Using 50% native bulk soil as inoculant in the potting mix significantly improved the survival rate of the cuttings compared to no-treated cuttings. Twenty-four plant growth-promoting rhizobacteria (PGPR) producing indole acetic acid (IAA) were isolated from the rhizosphere and roots of the survived snowbrush. Seventeen isolates had more than 10µg/mL of IAA were shortlisted and tested for seven different plant growth-promoting (PGP) traits; 76% showed nitrogen-fixing ability on Norris Glucose Nitrogen free media,70% showed phosphate solubilization activity, 76% showed siderophore production, 36% showed protease activity, 94% showed ACC deaminase activity on DF-ACC media, 76% produced catalase and all of isolates produced ammonia. Eight of seventeen isolates, CK-6, CK-22, CK-41, CK-44, CK-47, CK-50, CK-53, and CK-55, showed an increase in shoot biomass in Arabidopsis thaliana. Seven out of eight isolates were identified as Pseudomonas, except CK-55, identified as Sphingobium based on 16S rRNA gene sequencing. The shortlisted isolates are being tested on different grain and vegetable crops to mitigate drought stress and promote plant growth.

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Section: Methodsmentioning

confidence: 99%

“…After eight weeks, the cuttings were observed for rooting. The rooted cuttings were transplanted to 3.8 L pots (peat moss (75%), vermiculite (13%), rice husks (12%), wetting agent (AguaGro G, and hydrated lime- CaCO 3 ) ( Paudel et al., 2022 ). After ten months of transplantation of cuttings, the survival rate was recorded.…”

Section: Methodsmentioning

confidence: 99%

IAA-producing plant growth promoting rhizobacteria from Ceanothus velutinus enhance cutting propagation efficiency and Arabidopsis biomass

Ganesh,

Hewitt,

Devkota

et al. 2024

Front. Plant Sci.

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“…Two plants were not inoculated with native soil and were considered as control. Snowbrush ceanothus is a difficult plant to propagate in nursery conditions by cuttings ( Paudel et al., 2022 ). Cuttings are the preferred way to propagate plants to get uniform siblings compared to plants by seeds, so we had a limited number of plants for this study.…”

Section: Methodsmentioning

confidence: 99%

Exploration of the rhizosphere microbiome of native plant Ceanothus velutinus – an excellent resource of plant growth-promoting bacteria

et al. 2022

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Continuous demand for an increase in food production due to climate change and a steady rise in world population requires stress-resilient, sustainable agriculture. Overuse of chemical fertilizers and monoculture farming to achieve this goal deteriorated soil health and negatively affected its microbiome. The rhizosphere microbiome of a plant plays a significant role in its growth and development and promotes the plant’s overall health through nutrient uptake/availability, stress tolerance, and biocontrol activity. The Intermountain West (IW) region of the US is rich in native plants recommended for low water use landscaping because of their drought tolerance. The rhizosphere microbiome of these native plants is an excellent resource for plant growth-promoting rhizobacteria (PGPR) to use these microbes as biofertilizers and biostimulants to enhance food production, mitigate environmental stresses and an alternative for chemical fertilizer, and improve soil health. Here, we isolated, purified, identified, and characterized 64 bacterial isolates from a native plant, Ceanothus velutinus, commonly known as snowbrush ceanothus, from the natural habitat and the greenhouse-grown native soil-treated snowbrush ceanothus plants. We also conducted a microbial diversity analysis of the rhizosphere of greenhouse-grown native soil-treated and untreated plants (control). Twenty-seven of the 64 isolates were from the rhizosphere of the native region, and 36 were from the greenhouse-grown native soil-treated plants. These isolates were also tested for plant growth-promoting (PGP) traits such as their ability to produce catalase, siderophore, and indole acetic acid, fix atmospheric nitrogen and solubilize phosphate. Thirteen bacterial isolates tested positive for all five plant growth-promoting abilities and belonged to the genera Pantoea, Pseudomonas, Bacillus, and Ancylobacter. Besides, there are isolates belonging to the genus Streptomyces, Bacillus, Peribacillus, Variovorax, Xenophilus, Brevundimonas, and Priestia, which exhibit at least one of the plant growth-promoting activities. This initial screen provided a list of potential PGPR to test for plant health improvement on model and crop plants. Most of the bacterial isolates in this study have a great potential to become biofertilizers and bio-stimulants.

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Vegetative propagation ofCeanothus velutinususing stem cuttings

Cited by 2 publications

References 0 publications

IAA-producing plant growth promoting rhizobacteria from Ceanothus velutinus enhance cutting propagation efficiency and Arabidopsis biomass

IAA-producing plant growth promoting rhizobacteria from Ceanothus velutinus enhance cutting propagation efficiency and Arabidopsis biomass

Exploration of the rhizosphere microbiome of native plant Ceanothus velutinus – an excellent resource of plant growth-promoting bacteria

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