Vegetative compatibility groups within Fusarium oxysporum f.sp. lini from Linum usitatissimum (flax) wilt nurseries in western Canada

Mpofu, S. I.; Rashid, K. Y.

doi:10.1139/b01-058

Cited by 7 publications

(5 citation statements)

References 26 publications

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“…This indicates geographic variation in pathogen populations and virulence. Differences among fusarium wilt populations within Canada have also been found (Mpofu and Rashid, 2000). It would be useful to compare the results of this study with screenings of the same accessions in North America in order to detect differences in pathogen populations between North America and Russia.…”

Section: Discussionmentioning

confidence: 86%

Variation patterns within 153 flax (Linum usitatissimum L.) genebank accessions based on evaluation for resistance to fusarium wilt, anthracnose and pasmo

Diederichsen

Rozhmina

Kudrjavceva

2008

Plant Genet. Resour.

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Germplasm of 153 flax (Linum usitatissimum) accessions from 24 countries held at Plant Gene Resources of Canada (PGRC) was evaluated for resistance to fusarium wilt (Fusarium oxysporum), anthracnose (Colletotrichum lini) and pasmo (Septoria linicola). The screening was conducted at the All-Russian Flax Research Institute (VNIIL) at Torzhok, Russia, over 3 years for fusarium wilt and anthracnose, and over 2 years for pasmo. A disease severity index ranging from 0% (no infection) to 100% (heavy infection) was calculated based on observations after artificial inoculation with the pathogens in the greenhouse (fusarium wilt) or in field nurseries (anthracnose and pasmo). The average disease severity index for fusarium wilt was 56.6^34.4% (range 0-100.0%), for anthracnose 59.8^8.1% (range 43.8-83.9%) and for pasmo 74.2^11.8% (range 27.3-100.0%). The variation of disease severity indices among the years and within each accession was highest for fusarium wilt. Higher than average resistance for all three diseases was found in accessions from East Asia, while germplasm from the Indian subcontinent showed considerably lower than average resistance. Germplasm from North America and South America (mostly linseed) displayed above average resistance to fusarium wilt, while European accessions (mostly fibre flax) showed lower than average resistance to this disease. The different resistance levels reflected the improvements made by plant breeding and differences in the environments under which the germplasm accessions evolved. Accessions with potential use in linseed and fibre flax breeding were identified.

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Section: Discussionmentioning

confidence: 86%

Variation patterns within 153 flax (Linum usitatissimum L.) genebank accessions based on evaluation for resistance to fusarium wilt, anthracnose and pasmo

Diederichsen

Rozhmina

Kudrjavceva

2008

Plant Genet. Resour.

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“…At present, the Folini pangenome captures gene content from 4 clonal lineages. Yet, a survey of vegetative compatibility conducted on 74 isolates in wilt nurseries in western Canada identified 12 vegetative compatibility groups ( Mpofu and Rashid, 2001 ), thus emphasizing the need for further research, including sequencing of new strains from different regions.…”

Section: Discussionmentioning

confidence: 99%

Pangenomics of flax fungal parasite Fusarium oxysporum f. sp. lini

Logachev,

Kanapin,

Rozhmina

et al. 2024

Front. Plant Sci.

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To assess the genomic diversity of Fusarium oxysporum f. sp. lini strains and compile a comprehensive gene repertoire, we constructed a pangenome using 13 isolates from four different clonal lineages, each exhibiting distinct levels of virulence. Syntenic analyses of two selected genomes revealed significant chromosomal rearrangements unique to each genome. A comprehensive examination of both core and accessory pangenome content and diversity points at an open genome state. Additionally, Gene Ontology (GO) enrichment analysis indicated that non-core pangenome genes are associated with pathogen recognition and immune signaling. Furthermore, the Folini pansecterome, encompassing secreted proteins critical for fungal pathogenicity, primarily consists of three functional classes: effector proteins, CAZYmes, and proteases. These three classes account for approximately 3.5% of the pangenome. Each functional class within the pansecterome was meticulously annotated and characterized with respect to pangenome category distribution, PFAM domain frequency, and strain virulence assessment. This analysis revealed that highly virulent isolates have specific types of PFAM domains that are exclusive to them. Upon examining the repertoire of SIX genes known for virulence in other formae speciales, it was found that all isolates had a similar gene content except for two, which lacked SIX genes entirely.

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“…Vegetative compatibility analysis is a very useful tool for analysing pathogenic and nonpathogenic fungal populations and for identifying new races in crop production areas or regions or among host genotypes (Mpofu & Rashid, ; Krnjaja et al ., ). Because vegetatively compatible isolates have the same allele at each and every incompatibility locus, isolates of similar pathogenicity may belong to the same VCG (Dervis et al ., ; Krnjaja et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Heterokaryosis and diploid formation among Brazilian isolates of Macrophomina phaseolina

et al. 2018

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Heterokaryosis is the association of genetically distinct nuclei in a common hyphal cytoplasm, and is a process involved in the generation of fungal variation. The fusion of the distinct nuclei within a heterokaryotic hypha produces a heterozygous diploid nucleus and is one of the stages of the parasexual cycle. A heterokaryon's viability depends on the isolates’ genetic constitution with regard to loci called het (heterokaryon incompatibility) or vic (vegetative incompatibility). The current study evaluates for the first time the diversity of vegetative compatibility reactions in isolates of Macrophomina phaseolina from different hosts. Complementary nit (nitrate non‐utilizing) mutants of each isolate were obtained and paired in all possible combinations. Isolates were classified in vegetative compatibility groups (VCG) according to their ability to form viable heterokaryons. Ten VCGs were identified, two of them containing two isolates, and the remainder containing a single isolate. When grown on basal medium, heterokaryons produced both (i) auxotrophic segregants exhibiting the same phenotype as the paired mutants; and (ii) a fast‐growing sector, characterized as a heterozygous diploid sector, named D653, which showed a nit+ phenotype with a growth rate similar to the original wild isolate. When grown in the presence of benomyl, a haploidizing agent, D653 produced auxotrophic haploid segregants exhibiting the nit phenotypes of the crossed mutants. The results demonstrate for the first time the ability of M. phaseolina isolates to form viable heterokaryons and heterozygous diploid nuclei, suggesting that the parasexual cycle may be an alternative source of genetic variability in this species.

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Vegetative compatibility groups within Fusarium oxysporum f.sp. lini from Linum usitatissimum (flax) wilt nurseries in western Canada

Cited by 7 publications

References 26 publications

Variation patterns within 153 flax (Linum usitatissimum L.) genebank accessions based on evaluation for resistance to fusarium wilt, anthracnose and pasmo

Variation patterns within 153 flax (Linum usitatissimum L.) genebank accessions based on evaluation for resistance to fusarium wilt, anthracnose and pasmo

Pangenomics of flax fungal parasite Fusarium oxysporum f. sp. lini

Heterokaryosis and diploid formation among Brazilian isolates of Macrophomina phaseolina

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