Vegetative Compatibility Groups and Subgroups in<i>Fusarium oxysporum</i>f. sp.<i>radicis-lycopersici</i>

Katan, Talma; Zamir, Daniel; Sarfatti, M.; Katan, J.

doi:10.1094/phyto-81-255

Cited by 104 publications

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“…During VCG testing, all compatibility reactions were easily discernible. There was no evidence of the self-incompatibility reported in other studies (Correll et al, 1986b;Jacobson and Gordon, 1988), nor were there any weak reactions as has been observed in other studies (Elmer and Stephens, t989;Gordon and Okamoto, 1991;Katan et al, 1991). The isolates sorted into 23 VCGs arbitrarily named A to W ( Table 1).…”

Section: Fusarium Oxysporum Schlecht Causes Vascular Wiltmentioning

confidence: 71%

Characterisation ofFusarium oxysporum isolated from carnation in Australia based on pathogenicity, vegetative compatibility and random amplified polymorphic DNA (RAPD) assay

et al. 1996

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Isolates of F. oxysporum collected from symptomless carnation cuttings from Australian carnation growers properties, together with isolates from national collections, were screened for pathogenicity and grouped according to vegetative compatibility and random amplified polymorphic DNA (RAPD) patterns. The collection of 82 Australian isolates sorted into 23 different vegetative compatibility groups (VCGs). Of 69 isolates tested for pathogenicity, 24 were pathogenic to carnations, while the remaining 45 were non-pathogenic. All pathogenic isolates were within two VCGs, one of which was also compatible with an isolate obtained from an international culture collection, and which is known to represent VCG 0021 and race 2. Race status of the two pathogenic VCGs remains unknown. The RAPD assay revealed distinct DNA banding patterns which could distinguish pathogenic from non-pathogenic isolates as well as differentiate between isolates from the two pathogenic VCGs. Fusarium oxysporumSchlecht. causes vascular wilt in a broad range of host plants including carnations (Dianthus caryophyllus L.). This species of Fusarium

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Section: Fusarium Oxysporum Schlecht Causes Vascular Wiltmentioning

confidence: 71%

Characterisation ofFusarium oxysporum isolated from carnation in Australia based on pathogenicity, vegetative compatibility and random amplified polymorphic DNA (RAPD) assay

et al. 1996

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“…The objective of this study was to determine genetic relatedness among the populations of C. gloeosporioides from almond, and between the almond pathotype and other pathotypes of Colletotrichum. The study was based on previous experience with pathogenicformae speciales of Fusarium oxysporum (Katan et al, 1991, using nitrate-nonutilizing (nit) mutants to test vegetative compatibility, as suggested by Puhalla (1985). Seventy isolates of C. gloeosporioides from almond, originating from 11 sites at two geographic regions, were used in this study.…”

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confidence: 99%

“…Generally, each plate was inoculated with three mutants in a triangle formation, and incubated at 20-22 ~ Complementation was usually evident within 10-15 days by the formation of a dense aerial wild-type mycelium where mycelia from two mutants met and formed a prototrophic heterokaryon (Brooker et al, 1991;Katan et al, 1991). Absence of wild-type growth at the contact zone between two nit mutants of the same parent isolate indicated allelic, overlapping, or otherwise non-complementary mutations, or vegetative self-incompatibility (Brooker et al, 1991).…”

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confidence: 99%

Vegetative compatibility among isolates ofColletotrichum gloeosporioides from almond in Israel

Katan

Shabi

1996

Eur J Plant Pathol

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Anthracnose, caused by Colletotrichum gloeosporioides, is the major disease of almond in Israel. Pathogen attack of young fruit results in fruit rot and leaf wilting. Seventy isolates of C. gloeosporioides were obtained from affected almond fruits collected at 11 sites during 1991-2 and 1994. Chlorate-resistant nitrate-nonutilizing (nit) mutants were generated from each isolate and used in complementation (heterokaryon) tests. The formation of complementary stable heterokaryons between mutants from different isolates showed that all the isolates belonged to a single vegetative compatibility group. Representative isolates of C. gloeosporioides from almond did not form heterokaryons with local isolates of Colletotrichum from anemone and avocado, indicating that the almond isolates constitute a distinct subspecific group within C. gloeosporioides.

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“…In our compatibility tests, weak heterokaryotic reactions were not generally observed among Japanese isolates (Table 5). These isolates may be "bridging strains" (Katan et al, 1991;Rowe, 1995), in which complete genetic isolation has not yet developed. These isolates of "bridging strains" were not concentrated in a specific pathotype but seemed to be related to the tomato pathotype, including the tomato wilt race 2 (Table 1).…”

Section: Discussionmentioning

confidence: 99%

How do Japanese isolates of Verticillium dahliae correspond with standardized VCG testers?

et al. 1999

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We examined the vegetative compatibility of 56 Japanese isolates provisionally assigned to four subgroups of V, dahliae to estimate the genetic relatedness with testers of the standardized VCGs. Subgroup J1 was assigned to VCG 2A/B as a new category of assignment. Subgroup J2, except isolate Vdt 110, was assigned to VCG 2A, and subgroup J3, except isolate Vdf 1, was assigned to VCG 2B. Isolates Vdf 1 and Vdt 110 were assigned to VCG 2A/B. Subgroup J4 was assigned to two subgroups, VCG 4B for Vde 1 and VCG 4A/B for FY 3 and HR 1. Four isolates were compatible with both VCG 2 and 4. Isolate U56 was compatible with VCG 2A/B and 4A. Isolates of VCG 2A, Vdt 9 and FF 1, were compatible with either VCG 4A or 4A/B. One isolate of VCG 2B, Vdp-4, was compatible with VCG 4A. Three isolates of subgroup J2 showed weak reactions with the testers of VCG 4. These isolates may be "bridging strains". Japanese isolates were composed of two VCGs, 2 and 4, "bridging strains" compatible with these VCGs, and some self-incompatible isolates. Testers of VCG 1 and VCG 3 did not show any reactions with the Japanese isolates.Key Words bridging strain; pathotype; standardized tester; VCG; Verticillium dahliae.Verticillium dahliae Kleb. is one of the important soilborne fungal plant pathogens. It infects a wide range of crops. As the pathogenicity depends upon the isolate (Bewley, 1922;Kendrick and Middleton, 1959), inoculation tests are required to determine the pathogenicity. In Japan, a differential host method has been proposed (lijima, 1983) and improved to determine certain host ranges (Hagiwara, 1990;Horiuchi et al., 1990;Suwa et al., 1987). However, this differential host method has not proven conclusive. Over time, as new differential hosts were used for the pathogenicity test, new categorizations resulted (lijima, 1983;Takeda et al., 1988). Recently, V, dahliae race-2 of tomato wilt (Kobayashi et al., 1989) and tomato-pepper pathotype of V. dahliae (lijima, 1983;Oshima et al., 1993) appeared as new diseases in Japan. Now, determination of pathotypes of V. dahliae has become quite complicated using the differential host method.On the other hand, genetic relatedness in fungi can be estimated by their vegetative compatibility (Glass and Kuldau, 1992;Leslie, 1993 Puhalla and Hummel (1983). By this method, the number of distinct VCGs was reduced from 16 to 4. Several authors have followed this system (Bao et al., 1998;Daayf et al., 1995;Dobinson et al., 1998;Elena, 1997;Korolev, et al., 1997aKorolev, et al., , 1997bRataj-Guranowska and Hiemsta, 1997;Strausbaugh et al., 1992;Strausbaugh, 1993). It should be emphasized that the method used in VCG analysis can affect the results significantly (Rowe, 1995).Evaluation of weak reactions led to subdivisions of VCGs 2 and 4. Joaquim and Rowe (1991) discussed differential reactions among isolates of VCG 2 but avoided the subdivision of this VCG in order to keep their VCG system for V. dahliae as simple as possible. In the case of VCG 4, three subgroups (4A, 4B, and 4A/B) were proposed to accomod...

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Vegetative Compatibility Groups and Subgroups inFusarium oxysporumf. sp.radicis-lycopersici

Cited by 104 publications

References 0 publications

Characterisation ofFusarium oxysporum isolated from carnation in Australia based on pathogenicity, vegetative compatibility and random amplified polymorphic DNA (RAPD) assay

Characterisation ofFusarium oxysporum isolated from carnation in Australia based on pathogenicity, vegetative compatibility and random amplified polymorphic DNA (RAPD) assay

Vegetative compatibility among isolates ofColletotrichum gloeosporioides from almond in Israel

How do Japanese isolates of Verticillium dahliae correspond with standardized VCG testers?

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