Vegetative compatibility grouping in Verticillium nigrescens and V. tricorpus

Korolev, Nadia; Katan, Talma

doi:10.1017/s0953756298006844

Cited by 18 publications

(23 citation statements)

References 29 publications

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“…We can thus rule out contaminated seed from California as the source for the Verticillium wilt outbreak of lettuce investigated here. Another important finding of this study is the high diversity within V. tricorpus, in agreement with Korolev and Katan (1999), who found considerably more vegetative compatibility groups in V. tricorpus than in other Verticillium species. Future studies will focus on comparing the virulence of the Japanese and the California isolates, as well as on further exploring the genetic diversity within V. tricorpus.…”

Section: Discussionsupporting

confidence: 91%

Verticillium tricorpus causing lettuce wilt in Japan differs genetically from California lettuce isolates

et al. 2010

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In January 2002, Verticillium wilt of lettuce (Lactuca sativa L.) caused by Verticillium tricorpus occurred in upland paddy fields in Hyogo Prefecture for the first time in Japan. This fungal species was first isolated from lettuce in California, USA. In the present study, the genetic relationships between the American and Japanese isolates of V. tricorpus from lettuce were analyzed to determine whether the pathogen could have migrated to Japan from the USA, the major lettuce-seed supplier for Japan. Nucleotide sequences of the rDNA internal transcribed spacer regions, as well as the genes coding for translation elongation factor 1-alpha and RNA polymerase II were compared among American and Japanese V. tricorpus isolates from lettuce. The Japanese isolates of V. tricorpus were distinct from the American. Random amplified polymorphic DNA analyses also supported this conclusion. These results demonstrated that Verticillium wilt on lettuce caused by V. tricorpus in Japan was not related to the isolates causing the disease in California.

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Section: Discussionsupporting

confidence: 91%

Verticillium tricorpus causing lettuce wilt in Japan differs genetically from California lettuce isolates

et al. 2010

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“…Members of the same VCG can undergo hyphal fusion, with the potential for transferring nuclear and cytoplasmic elements. VCGs have been very useful for identifying clones of fungi that are largely asexual, and VCGs often correlate with pathogenicity and other traits (Korolev and Katan 1999;Korolev et al 2000Korolev et al , 2001. Direct tests of heterokaryon formation involve establishment of a stable prototrophic heterokaryon under conditions in which neither of the two complementary auxotrophic mutants could survive or form wild-type growth.…”

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confidence: 99%

Vegetative compatibility grouping in Botrytis cinerea using sulphate non-utilizing mutants

2008

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Twenty-one strains of Botrytis cinerea isolated from six plant species on ten sites throughout Israel, as well as a strain from France, were tested for vegetative and mycelial incompatibility, pathogenicity, resistance to the fungicides carbendazim and iprodione, and colony morphology. Selenate-resistant mutants were isolated from the strains as spontaneous, fastgrowing sectors arising from restricted colonies on medium amended with sodium selenate with a mean frequency of 0.04 sectors/colony; 81% of the sectors were sulphate non-utilizing (sul) mutants. One hundred and four sul mutants were divided into two complementary groups: resistant (66 mutants) and sensitive to chromate. Based on compatibility reactions between chromate-resistant and chromate-sensitive sul mutants, 12 strains were compatible only with themselves and were each classified as belonging to different vegetative compatibility groups (VCGs). Nine strains were each compatible with one to three other strains, and were assembled into three multi-member VCGs. Mycelial incompatibility between wild-type strains (barrage), in the form of a zone of dark pigmentation or sparse mycelium with or without dark pigmentation of the agar along the line of confrontation, was observed for 70% of the inter-strain pairings. There was no correspondence in compatibility between strains revealed by two approaches: strains in different VCGs did not necessarily produce a barrage. However, self-compatibility was observed both as heterokaryon formation between complementary sul mutants and as an absence of barrages between mycelia of wild-type strains; wild-type strains belonging to the same VCG did not exhibit strong barrages, although weak antagonistic reactions were observed. Strains in two multimember VCGs showed the same patterns of resistance to carbendazim and iprodione; the third multi-member VCG contained isolates with different patterns of resistance. Four morphological types were revealed among wild-type strains: conidial (five strains), sclerotial (six strains), intermediate (ten strains), and mycelial (one strain). On bean leaves, conidial strains were more aggressive than sclerotial strains.

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“…In this experiment, although both potato isolates showed no external and internal symptoms with inoculated plants, reisolation rates were quite high. Similarly, V. tricorpus was reisolated from eggplant, snapdragon, tobacco, lupin, and cotton without showing any symptoms (Isaac 1953;Skotland 1971;Huisman 1988;Korolev and Katan 1999). These data indicate that V. tricorpus can readily colonize several plants.…”

Section: Discussionmentioning

confidence: 75%

“…tricorpus was isolated from tomato, potato, mint, cantaloupe, cotton, and weeds (Isaac 1953;Isaac and Harrison 1968;MacGarvie and Hide 1966;Skotland 1971;Huisman 1988;Korolev and Katan 1999). Also, this fungus has been reported as the wilt pathogen of tomato (Isaac 1953;Uys et al 1997) and snapdragon (Isaac 1956), and as the causal agent of dry rot of stored potato (Thanassoulopoulos and Giapanoglou 1994).…”

Section: Discussionmentioning

confidence: 99%

First report of Verticillium tricorpus isolated from potato tubers in Japan

et al. 2003

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In 1998, Verticillium sp. (CE98Vt1 and CE98Vt2) were isolated from discolored vascular structures of potato tubers sold at a market in Chiba Prefecture. These isolates were identified as Verticillium tricorpus on the basis of cultural and morphological characteristics and PCR diagnosis. This observed vascular discoloration of the potato tuber was demonstrated in three cultivars (Touya, Toyoshiro, and Waseshiro) among eight cultivars by inoculation to seedlings. External and internal symptoms of these isolates were not distinct in potato plants. The virulence of these isolates to potato was very low as compared with Verticillium dahliae. These two isolates were not pathogenic to Chinese cabbage, eggplant, green pepper, larkspur, parsley, snapdragon, soybean, tobacco, and tomato. This is the first report of V. tricorpus from potato in Japan.

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Vegetative compatibility grouping in Verticillium nigrescens and V. tricorpus

Cited by 18 publications

References 29 publications

Verticillium tricorpus causing lettuce wilt in Japan differs genetically from California lettuce isolates

Verticillium tricorpus causing lettuce wilt in Japan differs genetically from California lettuce isolates

Vegetative compatibility grouping in Botrytis cinerea using sulphate non-utilizing mutants

First report of Verticillium tricorpus isolated from potato tubers in Japan

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