Vegetables

Klocke, Evelyn; Nothnagel, Thomas; Schumann, G.

doi:10.1007/978-3-642-02391-0_25

Cited by 9 publications

(15 citation statements)

References 369 publications

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“…Recently, its effect has been characterized in a different genetic background also in combination with other relevant genes ( Pino et al, 2010 ; Lombardi-Crestana et al, 2012 ). By contrast, although some positive results have been reported ( Klocke et al, 2010 ), pepper ( Capsicum spp.) is considered a recalcitrant species due to several issues that jeopardize the in vitro response ( Kothari et al, 2010 ).…”

Section: Regeneration and Transformationmentioning

confidence: 94%

“…Successful transformation, however, relies on various phases, being the introduction and integration of DNA into the plant genome as well as the selection and regeneration of transformed cells the most important and, sometimes, limiting factors ( Wȩdzony et al, 2014 ; Altpeter et al, 2016 ). Regeneration of transformed plants has been extensively pursued in vegetable crops, although large differences in efficiency among families, species, and cultivars have been reported ( Klocke et al, 2010 ). Plant regeneration is generally achieved via in vitro culture systems, using a range of explants and following two alternative pathways: de novo shoot organogenesis (DNSO) or somatic embryogenesis (SE; Wȩdzony et al, 2014 ).…”

Section: Regeneration and Transformationmentioning

confidence: 99%

“…Within the Solanaceae family, eggplant ( Solanum melongena ) and, to a lesser extent, tomato ( S. lycopersicum ) have been successfully subjected to genetic transformation with various purposes using different approaches ( Klocke et al, 2010 ). Regeneration in eggplant has been obtained either through SE or DNSO starting from protoplasts, tissue explants, microspores ( Rotino et al, 2014 ).…”

Section: Regeneration and Transformationmentioning

confidence: 99%

“…Raphanus sativus , another vegetable of the same family, is considered recalcitrant and only few reports are available. Advances in regeneration and transformation in this group of crops have been reviewed elsewhere ( Vinterhalter et al, 2007 ; Klocke et al, 2010 ; Sparrow et al, 2011 ; Park et al, 2012 ; Kumar and Srivastava, 2016 ). With the aim to avoid tissue culture systems, similarly to the related species Arabidopsis thaliana, in planta transformation systems based on floral dipping or flower bud microinjection have been also attempted in various genotypes ( Curtis and Nam, 2001 ; Sparrow et al, 2011 ).…”

Section: Regeneration and Transformationmentioning

confidence: 99%

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Genetic Transformation and Genomic Resources for Next-Generation Precise Genome Engineering in Vegetable Crops

2017

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In the frame of modern agriculture facing the predicted increase of population and general environmental changes, the securement of high quality food remains a major challenge to deal with. Vegetable crops include a large number of species, characterized by multiple geographical origins, large genetic variability and diverse reproductive features. Due to their nutritional value, they have an important place in human diet. In recent years, many crop genomes have been sequenced permitting the identification of genes and superior alleles associated with desirable traits. Furthermore, innovative biotechnological approaches allow to take a step forward towards the development of new improved cultivars harboring precise genome modifications. Sequence-based knowledge coupled with advanced biotechnologies is supporting the widespread application of new plant breeding techniques to enhance the success in modification and transfer of useful alleles into target varieties. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 system, zinc-finger nucleases, and transcription activator-like effector nucleases represent the main methods available for plant genome engineering through targeted modifications. Such technologies, however, require efficient transformation protocols as well as extensive genomic resources and accurate knowledge before they can be efficiently exploited in practical breeding programs. In this review, we revise the state of the art in relation to availability of such scientific and technological resources in various groups of vegetables, describe genome editing results obtained so far and discuss the implications for future applications.

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Section: Regeneration and Transformationmentioning

confidence: 94%

Section: Regeneration and Transformationmentioning

confidence: 99%

Section: Regeneration and Transformationmentioning

confidence: 99%

Section: Regeneration and Transformationmentioning

confidence: 99%

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Genetic Transformation and Genomic Resources for Next-Generation Precise Genome Engineering in Vegetable Crops

2017

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“…However, the licence to produce F 1 hybrids using this system is no longer valid, and the marketing of these transgenic plants is not allowed in the European Union (Klocke et al. ).…”

Section: Types Of Pollination Controlmentioning

confidence: 99%

The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review

Colombo¹,

Galmarini

2017

Plant Breeding

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Production of hybrid varieties of vegetable crops is currently a desired breeding goal, due to their remarkable agronomic performance and to the possibility of intellectual property protection. However, efficient hybrid production requires a careful pollination control to guarantee the hybrid nature of F 1 seed. Several technologies ranging from manual emasculation to genetic transformation are used to inhibit pollen production in mother plants. In this review, we examine the principles underlying strategies like genetically determined systems (genic male sterility, cytoplasmic-genic male sterility, self-incompatibility) and other methods (manual emasculation, chemical-hybridizing agents) in different species, considering the benefits and drawbacks of their adoption. Finally, we present the current state of the art for vegetable hybrid seed production.

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Root-Knot Nematodes (Meloidogyne spp.): Biology, Plant-Nematode Interactions and Their Environmentally Benign Management Strategies

Khan

Ali

et al. 2023

Gesunde Pflanzen

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Vegetables

Cited by 9 publications

References 369 publications

Genetic Transformation and Genomic Resources for Next-Generation Precise Genome Engineering in Vegetable Crops

Genetic Transformation and Genomic Resources for Next-Generation Precise Genome Engineering in Vegetable Crops

The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review

Root-Knot Nematodes (Meloidogyne spp.): Biology, Plant-Nematode Interactions and Their Environmentally Benign Management Strategies

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