Background
Malaria, one of the world’s greatest public health challenges, is an endemic disease with stable transmission in Togo. Combating malaria requires an effective vector control. This study provides temporal data on insecticide resistance status in the major malaria vector
Anopheles gambiae
sensu lato (s.l.) from Togo.
Methods
Two to 5 days old females of
An. gambiae
s.l., originating from three localities (Baguida, Kovié, Kolokopé) were subjected to insecticide-impregnated papers during 3 years (2012, 2013, 2016) as follows: organochlorides (4% DDT), pyrethroids (0.05% deltamethrin, 0.75% permethrin, 0.05% lambdacyhalothrin), carbamates (0.4% bendiocarb and 0.1% propoxur), and organophosphates (5% malathion, 0.4% chlorpyrifos methyl, 1% fenitrothion) following the WHO standard protocol. Dead and surviving mosquitoes were stored separately in Eppendorf tubes containing silica gel for DNA extraction, species identification, and
kdr
and
ace
-
1
genotyping.
Results
Knockdown times (KDT
50
and KDT
95
) were high in
An. gambiae
s.l. The lowest KDTs were recorded at Baguida in 2013 for deltamethrin (KDT
50
= 24.7, CI [22.4–27.12] and KDT
95
= 90.78, CI [76.35–113.49]). No KDTs were recorded for DDT and in some instances for permethrin. In general,
An. gambiae
s.l. was resistant to most of the four classes of insecticides during the survey periods regardless of locality and year, except to chlorpyrifos methyl. In some instances, mosquitoes were fully susceptible to fenitrothion (Kolokopé: 100% and Kovié: 98.05%, CI [95.82–100.26]) and malathion (100% at both Kolokopé and Kovié) in 2013, and malathion only (Kolokopé; 100%) in 2016.
Anopheles coluzzii
,
An. gambiae
and
Anopheles arabiensis
were the three sibling species identified at the three localities with some hybrids at Baguida (2013), and Kovié (2012 and 2016), respectively.
Anopheles gambiae
was relatively dominant (61.6%). The
kdr
1014F allele frequency was > 0.9 in most of the cases, except at Kolokopé (f (1014F) = 0.63, CI [0.55–0.71]) in 2013. The
kdr
1014S allele frequency was below 0.02. The highest
ace
-
1
frequencies were identified in
An. gambiae
at Baguida (2012: 0.52, CI [0.34–0.69] and 2013: 0.66, CI [0.46–0.86]).
Conclusion
The resistance status is worrying in Togo and should be considered in future malaria vector resistance management programmes by decision-makers.
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