Vegetable oils as NAPLs in two phase partitioning bioreactors for the degradation of anthracene by laccase

Arca-Ramos, A.; Eibes, Gemma; Moreira, Marı́a Teresa; Feijoó, Gumersindo; Lema, Juan M.

doi:10.1016/j.cej.2013.11.076

Cited by 20 publications

(4 citation statements)

References 29 publications

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“…In fact, the increase in the dissolved oxygen concentration was reported to improve the transformation of pollutants by laccase (Arca-Ramos et al 2014). In experiments 1-4 (Table 2), aeration was not considered because agitation was sufficient to maintain the level of dissolved oxygen close to saturation values (6.5-7.5 mg O 2 /L).…”

Section: Effect Of Hydraulic Retention Timementioning

confidence: 97%

Potentiality of a ceramic membrane reactor for the laccase-catalyzed removal of bisphenol A from secondary effluents

Arca-Ramos

Eibes

Feijoo

et al. 2015

Appl Microbiol Biotechnol

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In this study, the removal of bisphenol A (BPA) by laccase in a continuous enzymatic membrane reactor (EMR) was investigated. The effects of key parameters, namely, type of laccase, pH, and enzyme activity, were initially evaluated. Once optimal conditions were determined, the continuous removal of the pollutant in an EMR was assessed in synthetic and real biologically treated wastewaters. The reactor configuration consisted of a stirred tank reactor coupled to a ceramic membrane, which prevented the sorption of the pollutant and allowed the recovery and recycling of laccase. Nearly complete removal of BPA was attained under both operation regimes with removal yields above 94.5 %. In experiments with real wastewater, the removal of BPA remained high while the presence of colloids and certain ions and the formation of precipitates on the membrane potentially affected enzyme stability and made necessary the periodic addition of laccase. Polymerization and degradation were observed as probable mechanisms of BPA transformation by laccase.

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Section: Effect Of Hydraulic Retention Timementioning

confidence: 97%

Potentiality of a ceramic membrane reactor for the laccase-catalyzed removal of bisphenol A from secondary effluents

Arca-Ramos

Eibes

Feijoo

et al. 2015

Appl Microbiol Biotechnol

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“…13 Regarding the specific performance of laccase in biphasic systems, this enzyme has been used in the degradation of polluting aromatic compounds (namely estradiol and anthracene), observing an increase in substrate solubility that consequently promoted mass transfer, enhancing the overall substrate conversion. 13,14 Thus, these works suggest that the laccase-catalyzed oxidation of hydrophobic substrates can be positively affected by the presence of a more hydrophilic phase, where laccase is preferentially solubilized, and a hydrophobic phase that enhances the substrate solubility.…”

Section: ■ Introductionmentioning

confidence: 99%

Integrated Biocatalytic Platform Based on Aqueous Biphasic Systems for the Sustainable Oligomerization of Rutin

Muñiz-Mouro

Ferreira

Coutinho

et al. 2021

ACS Sustainable Chem. Eng.

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Rutin is a known antioxidant compound that displays a broad range of biological activities and health-related benefits but presents a low water solubility that can be overcome by its polymerization. In this work, biocompatible aqueous biphasic systems composed of the ionic liquid cholinium dihydrogen phosphate ([CH][DHph]) and the polymer poly-(ethylene glycol) 600 (PEG 600) were investigated as an efficient integrated reaction−separation platform for the laccase-catalyzed oligomerization of rutin. Two different approaches were studied to reuse laccase in several oligorutin production cycles, the main difference between them being the use of monophasic or biphasic regimes during the oligomerization reaction. The use of a biphasic regime in the second approach (heterogeneous reaction medium) allowed the successful reuse of the biocatalyst in three consecutive reaction−separation cycles while achieving noteworthy rutin oligomerization yields (95% in the first cycle, 91% in the second cycle, and 89% in the last cycle). These remarkable results were caused by the combination of the increased solubility of rutin in the PEGrich phase together with the enhanced catalytic performance of laccase in the [Ch][DHph]-rich phase, alongside with the optimization of the pH of the reaction medium straightly linked to enzyme stability. Finally, a life-cycle assessment was performed to compare this integrated reaction−separation platform to three alternative processes, reinforcing its sustainability.

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“…The selection of a NAP must satisfy several criteria [5]. Among the liquid phases tested (e.g., lubricant [6], vegetal oils [6,7], alkanes [8,9], ionic liquids [10]), silicone oils (i.e., PolyDiMethylSiloxane, PDMS) are the most adapted NAP to be implemented in a TPPB (mainly due to their low volatility and viscosity, high affinity for VOC, compatibility with biomass, and no bioavailability) [11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Removal of a Mixture of Seven Volatile Organic Compounds (VOCs) Using an Industrial Pilot-Scale Process Combining Absorption in Silicone Oil and Biological Regeneration in a Two-Phase Partitioning Bioreactor (TPPB)

et al. 2022

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The treatment of a synthetic polluted gas containing seven volatile organic compounds (VOCs) was studied using a pilot plant in real industrial conditions. The process combined VOC absorption in silicone oil (PolyDiMethylSiloxane, i.e., PDMS), a biological regeneration of the PDMS in a two-phase partitioning bioreactor (TPPB), and a phase separation including settling and centrifugation. The TPPB was operated at a water/PDMS volume ratio of 75/25. The VOCs treatment performance was efficient during the entire test, corresponding to 10 PDMS regeneration cycles. The analysis of the content of the aqueous phase and PDMS confirmed that VOCs are progressively degraded until mineralization. The nitrogen consumption and the characterization of the microorganisms highlighted possible anoxic functioning of the biomass within the first decanter. Moreover, although the absorption and biodegradation performances were very satisfactory, the separation of all phases, essential for the PDMS recycling, was problematic due to the production of biosurfactants by the microorganisms, leading to the formation of a stable emulsion and foaming episodes. As a consequence, the packed column showed slight fouling. However, no significant increase in the pressure drop of the packed bed, as well as no significant impact on VOC absorption efficiency was observed.

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Vegetable oils as NAPLs in two phase partitioning bioreactors for the degradation of anthracene by laccase

Cited by 20 publications

References 29 publications

Potentiality of a ceramic membrane reactor for the laccase-catalyzed removal of bisphenol A from secondary effluents

Potentiality of a ceramic membrane reactor for the laccase-catalyzed removal of bisphenol A from secondary effluents

Integrated Biocatalytic Platform Based on Aqueous Biphasic Systems for the Sustainable Oligomerization of Rutin

Removal of a Mixture of Seven Volatile Organic Compounds (VOCs) Using an Industrial Pilot-Scale Process Combining Absorption in Silicone Oil and Biological Regeneration in a Two-Phase Partitioning Bioreactor (TPPB)

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