Vectors to achieve selective expression of vaccine antigens within eukaryotic cells using Salmonella spp. as carrier strains

Basso, Holger

doi:10.1016/s0378-1097(99)00593-5

Cited by 2 publications

(2 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to their propensity to infect APCs, facultative intracellular bacteria are ideal carriers for heterologous antigens and the elicitation of cellular immune responses. Efficient protection against infectious agents and tumors have been achieved by using Salmonella as a DNA carrier [89][90][91][92][93][94][95]. Interestingly, in vivo studies demonstrated that DCs can be specifically transfected by using this approach [96].…”

Section: Dna Vaccination: Using the Classical Or The Alternative Mhc mentioning

confidence: 99%

Rational Design of Vaccination Strategies to Promote Antigen Entry into the MHC Class I-Restricted Presentation Pathway

Becker¹,

Guzmán²

2004

Transfus Med Hemother

View full text Add to dashboard Cite

Cytotoxic CD8+ T lymphocytes (CTLs) constitute one of the main effector mechanisms against tumors and viral infections. CTLs specifically recognize short peptides (8-10 residues long) displayed on the surface of ‘target’ cells, which result from the processing of foreign or abnormal proteins (e.g. virus and tumor proteins) and are bound to major histocompatibility complex (MHC) class I molecules. Virtually all nucleated cells display on their surface fragments of intracellularly produced polypeptides. When there are signs of invasion or transformation, CTLs take control of the situation by destroying these ‘labeled’ target cells. This is an extremely efficient mechanism. However, the efficient differentiation of naïve CD8+ T cells into CTLs is a limiting prerequisite. To achieve this differentiation, dendritic cells (DCs) are critical since only these professional antigen-presenting cells (APCs) can provide not only the peptide presented onto the MHC class I molecules but also the costimulatory signals required for this activation. To this end, DCs take up antigens and degrade them into peptides which are loaded on MHC class I and presented onto the surface to prime specific T lymphocytes. In this review, we summarize the current knowledge on the mechanisms used by professional APCs in the processing and presentation of endogenous and exogenous antigens in the context of MHC class I molecules (i.e. priming and cross-priming). We will also discuss new vaccination strategies that take advantage of these physiological mechanisms in order to improve the elicitation of cytotoxic responses to eliminate intracellular pathogens and tumors.

show abstract

Section: Dna Vaccination: Using the Classical Or The Alternative Mhc mentioning

confidence: 99%

Rational Design of Vaccination Strategies to Promote Antigen Entry into the MHC Class I-Restricted Presentation Pathway

Becker¹,

Guzmán²

2004

Transfus Med Hemother

View full text Add to dashboard Cite

show abstract

“…More recently, it was demonstrated that, by integrating a single copy of a heterologous gene associated with nirB promoter into attenuated Salmonella chromosome, an inducible expression of foreign antigen was achieved [23]. A similar approach relies on chromosomal insertion of heterologous genes under the control of in vivo-inducible promoters that allow a selective expression within human macrophages by bacterial carriers Salmonella enterica serovar Typhi [24,25].…”

Section: Introductionmentioning

confidence: 99%

Identification of Salmonella enterica Serovar Typhi DNA Fragments with Transcriptional Activity Under Different Growth Conditions

Saverino¹,

McDermott²,

Ferrari³

et al. 2008

TOIDJ

View full text Add to dashboard Cite

Salmonella enterica serovar Typhi is a human pathogenic microorganism with a very complex infective cycle, involving the transit of bacteria across different microenvironments; to optimize the performance of attenuated Salmonella strains suitable as live carriers of heterologous antigens, fine tuning of wild type bacteria gene expression is essential.Several DNA fragments were obtained from a Salmonella enterica serovar Typhi (vi+, fim+) blood isolate and 18 clones were selected according to the dimension of the insert (range <0.2-1.6 kb). These fragments showed a transcriptional activity in a promoterless vector cloned in Escherichia coli background, according to homogeneous parameters. The results obtained provide an insight about signals mediating gene activation in vivo, particularly in the microenvironments known to exist during the infectious process, even if the fragments are not promoters sequences. Finally, the functional characterization of several fragments showed that they possessed an efficient and homogeneous transcriptional activity, worth to be further investigated.

show abstract

Vectors to achieve selective expression of vaccine antigens within eukaryotic cells using Salmonella spp. as carrier strains

Cited by 2 publications

References 7 publications

Rational Design of Vaccination Strategies to Promote Antigen Entry into the MHC Class I-Restricted Presentation Pathway

Rational Design of Vaccination Strategies to Promote Antigen Entry into the MHC Class I-Restricted Presentation Pathway

Identification of Salmonella enterica Serovar Typhi DNA Fragments with Transcriptional Activity Under Different Growth Conditions

Contact Info

Product

Resources

About