Vectors and Gene Therapy

Burton, Edward A.; Fink, David J.

doi:10.1002/3527600906.mcb.200400087

Cited by 2 publications

(3 citation statements)

References 215 publications

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“…Finally, gene expression changes that appear neuroprotective in models of sporadic PD could be developed as possible gene therapy approaches. The most effective gene delivery vehicles in the CNS in vivo are recombinant virus vectors, in which viral genes mediating pathogenic functions are replaced with transgene sequences of interest (Burton, et al, 2005). A variety of viral vectors has been used to deliver transgenes to the substantia nigra in vivo .…”

Section: Introductionmentioning

confidence: 99%

Pseudotype-dependent lentiviral transduction of astrocytes or neurons in the rat substantia nigra

Cannon

Sew

Montero

et al. 2011

Experimental Neurology

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Gene transfer to the central nervous system provides powerful methodology for the study of gene function and gene-environment interactions in vivo, in addition to a vehicle for the delivery of therapeutic transgenes for gene therapy. The aim of the present study was to determine patterns of tropism exhibited by pseudotyped lentiviral vectors in the rat substantia nigra, in order to evaluate their utility for gene transfer in experimental models of Parkinson’s disease. Isogenic lentiviral vector particles encoding a GFP reporter were pseudotyped with envelope glycoproteins derived from vesicular stomatitis virus (VSV), Mokola virus (MV), lymphocytic choriomeningitis virus (LCMV), or Moloney murine leukemia virus (MuLV). Adult male Lewis rats received unilateral stereotactic infusions of vector into the substantia nigra; three weeks later, patterns of viral transduction were determined by immunohistological detection of GFP. Different pseudotypes gave rise to transgene expression in restricted and distinct cellular populations. VSV and MV pseudotypes transduced midbrain neurons, including a subset of nigral dopaminergic neurons. In contrast, LCMV and MuLV-pseudotyped lentivirus produced transgene expression exclusively in astrocytes; the restricted transduction of astroglial cells was not explained by the cellular distribution of receptors previously shown to mediate entry of LCMV or MuLV. These data suggest that pseudotyped lentiviral vectors will be useful for experimental gene transfer to the rat substantia nigra. In particular, the availability of neuronal and astrocytic-targeting vectors will allow dissociation of cell-autonomous and cell non-autonomous functions of key gene products in vivo.

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Section: Introductionmentioning

confidence: 99%

Pseudotype-dependent lentiviral transduction of astrocytes or neurons in the rat substantia nigra

Cannon

Sew

Montero

et al. 2011

Experimental Neurology

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“…In order to properly understand the departmentalization of the administrative structure, we will examine the following. (Burton. 2006) Departmentalization means to divide the activities and employees in departments in order to improve the organization.…”

Section: Grouping Jobsmentioning

confidence: 99%

“…The process of combining these functions and creating separate departments is called departmentalization. (Burton. 2006) To achieve the goals of an organization, the division of the structure of the organization into other departments is called departmentalization.…”

Section: Introductionmentioning

confidence: 99%

Exploring Jobs Design and Grouping Jobs Elements in Managerial Organizing, an Identification and Explanation

Khalili

2024

Integr. J. Res. Arts Humanities

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Managerial Summary: Human is a social being. Sociability and living together with other people in society is now a very important aspect of human life. From the long stages of human history, an important part has emerged that man needs organizations in the society to ensure the needs of his life. These organizations are at a higher standard than before, good examples are the government, intelligence agencies, commercial companies and many other executive bodies. According to the need of the present life, human is obliged to have a better organizational process to organize these organizations and implement strategic plans. So that everything is done according to the need and goals are achieved. Therefore, it's clear that humans have a serious need to organize organizations and the organizing process is very important. Research Summary: Organizing is a broad aspect of management science. In order to achieve the goals of any organization, the organize of employees, appropriate use of experience and skills of employees, close relationship and cooperation of employees, selection of employees and supervision of employees are the main pillars. After examining various scientific results in this research, there is a proper consideration of the above cases. The elements of Jobs Design and Grouping Jobs, have been explored in this article from vision of Management science.

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Vectors and Gene Therapy

Cited by 2 publications

References 215 publications

Pseudotype-dependent lentiviral transduction of astrocytes or neurons in the rat substantia nigra

Pseudotype-dependent lentiviral transduction of astrocytes or neurons in the rat substantia nigra

Exploring Jobs Design and Grouping Jobs Elements in Managerial Organizing, an Identification and Explanation

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