Vector Plasmids for in-Vivo and in-Vitro Manipulations of Gram-Negative Bacteria

Simon, Reinhard; Priefer, Ursula B.; Pühler, Alfred

doi:10.1007/978-3-642-69338-0_11

Cited by 341 publications

(164 citation statements)

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“…Plasmid pGC479 is a pSUP202 derivative (Simon et al, 1983) that carries a 2170 bp Hind111 fragment containing the nifA gene of R. etli CFN42 (GenBank accession no. U31630).…”

Section: Methodsmentioning

confidence: 99%

Transcriptional activity of the symbiotic plasmid of Rhizobium etli is affected by different environmental conditions

et al. 1996

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“…Plasmid pGC479 is a pSUP202 derivative (Simon et al, 1983) that carries a 2170 bp Hind111 fragment containing the nifA gene of R. etli CFN42 (GenBank accession no. U31630).…”

Section: Methodsmentioning

confidence: 99%

Transcriptional activity of the symbiotic plasmid of Rhizobium etli is affected by different environmental conditions

et al. 1996

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“…Transconjugant was as good as its parent as regards the P-solubilizing effi ciency (data not shown), suggesting that introduction of lacZ did not affect P solubilizing effi ciency. No other PSB was able to grow on TCP plates containing Km 50 Nx 25 , indicating that lacZ can be used as effective marker for PSB. No blue colonies were formed on LB plates containing Km 50 Nx 25 plus X-gal when soil dilutions from controls (without inoculation of lacZ marked Pseudomonas) were plated indicating that the enumeration method specifi cally identifi ed the lacZ marked strain.…”

Section: Resultsmentioning

confidence: 98%

“…P-36. The transconjugant P-36 produced blue colonies on X-Gal plates containing Km 50 Nx 25 . Insertion of lacZ gene was confi rmed by estimating the ß-galactosidase activity of parent as well as its exconjugant.…”

Section: Resultsmentioning

confidence: 99%

“…Simon [25] observed transposition frequency between 10 -3 to 10 -6 using pSUP and pUT vectors in different Gram-negative bacteria. The transposition frequency varies with recipient strain and growth conditions.…”

Section: Resultsmentioning

confidence: 99%

“…plasmid pSUP101 as described earlier [6].The exconjugants were screened on LB (Luria Bertini) plates containing nalidixic acid (Nx) (25 μg ml -1 ), and kanamycin (Km) (50 μg ml -1 ). Exconjugats obtained were purifi ed on Km 50 Nx 25 plates containing X-Gal (5,Bromo-4-chloro-3-indoyl-β-Dgalactopyranoside) 20 mg 1 -1 . Transconjugants containing lacZ gene in right orientation formed blue colonies on XGal plates.…”

Section: Introductionmentioning

confidence: 99%

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Establishment of lacZ marked strain of phosphate solubilizing bacterium in the rhizosphere and its effect on plant growth in mungbean

et al. 2010

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The establishment of lacZ marked strain of Psolubilizing bacterium Pseudomonas in the rhizosphere of mungbean (Vigna radiata) under pothouse conditions was studied. The lacZ marker was transferred to Pseudomonas P-36 on LB medium using donor strain of E. coli. The lacZ marked strain formed blue colonies on selective media and could be identifi ed from soil on the basis of this character. The lacZ marked strain was able to survive in rhizosphere of mungbean under pothouse conditions and maintained a population of about 10 4 g -1 of rhizosphere soils up to 60 days study period. Positive effect of inoculation with P-solubilizing bacterium on dry matter yield, P and N-uptake was observed using rock phosphate and single super phosphate as P sources with and without farmyard amendment.

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Biocontrol of Root Diseases byPseudomonas fluorescensCHA0: Current Concepts and Experimental Approaches

Voisard¹,

Bull

Keel³

et al. 1994

Molecular Ecology of Rhizosphere Microorganisms

197

165

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Certain strains of fluorescent pseudomonads can effectively colonize plant roots and protect plants from diseases caused by a variety of root pathogens. Such beneficial or plant health-promoting strains are emerging as promising biocontrol agents. They are suited as soil inoculants either individually or in combination and may be compatible with current chemical pesticides (1, 2, 3, 4, 5, 6, 7, 8). In our biocontrol studies, we have focused on Pseudomonasfluorescens strain CHAO, an isolate from a suppressive soil in the western part of Switzerland (9). This strain was originally shown to colonize tobacco roots and to suppress black root rot, which is caused by the fungus Thielaviopsis basicola (9, 10). Subsequent work has established that disease suppression by strain CHAO displays little specificity with respect to the host plant and the pathogen. Protected plants include wheat, cucumber, sugar beet, cotton, flax, corn, and cress. Pathogenic action of at least the following fungal pathogens can be reduced by strain CHAO: Pythium ultimum, Gaeumannomyces graminis var. tritici (Ggt), Fusarium oxysporum f.s p. cucurbitaceae, Phomopsis sclerotioides, and Rhizoctonia solani (11, 12, 13, 14, 15; our unpublished data). Since the interactions between I! fluorescens, other organisms and the soil environment are extremely complex, it became important to develop reproducible methods that allow us to monitor the plant-beneficial effects of strain CHAO reliably and to analyze the traits that make it an effective biocontrol agent. In section 6.2 we will review some of our approaches to investigate the mechanisms by which strain CHAO achieves biological control.We are using strain CHAO as a model organism to study not only the mechanisms of disease suppression but also the ecological impact of introduced plant-beneficial bacteria (see section 6.3). In a parallel approach, we are investigating the potential applications of biological control agents to improve the yields of protected crops. We are testing a variety of strains, singly and in combination, for the development of greenhouse applications. A brief account of this work is presented in section 6.4. 68Disease Suppression by I! Fluorescens CHAO Mechanistic Studies on Biocontrol m i t s of Pseudomonas Fluorescens CHAO Chemical Identification of Extracellular MetabolitesMetabolites produced and excreted by I? fluorescens are assumed to be important biotic factors in the biological control of root diseases (2, 5, 16, 17, 18, 19, 20, 21). Until now, about a dozen low molecular weight compounds have been identified in culture supernatants of Rfluorescens CHAO ( Table 1). These products can be broadly classified into two groups: siderophores and secondary metabolites. The siderophores (e. g. , iron chelators) pyoverdine (pseudobactin), salicylate and pyochelin are all produced by I? fluorexens CHAO (22, 23 ; our unpublished results) and by other fluorescent pseudomonads when these bacteria are grown under ironlimiting conditions (24; reviewed by Loper & Buyer [20] and O'Sullivan...

show abstract

Vector Plasmids for in-Vivo and in-Vitro Manipulations of Gram-Negative Bacteria

Cited by 341 publications

References 12 publications

Transcriptional activity of the symbiotic plasmid of Rhizobium etli is affected by different environmental conditions

Transcriptional activity of the symbiotic plasmid of Rhizobium etli is affected by different environmental conditions

Establishment of lacZ marked strain of phosphate solubilizing bacterium in the rhizosphere and its effect on plant growth in mungbean

Biocontrol of Root Diseases byPseudomonas fluorescensCHA0: Current Concepts and Experimental Approaches

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