Vector Incrimination of Sand Flies in the Most Important Visceral Leishmaniasis Focus in Iran

Oshaghi, Mohammad Ali; Maleki‐Ravasan, Naseh; Javadian, E; Mohebali, Mehdi; Hajjaran, Homa; Zare, Zabiholah; Mohtarami, Fatemeh; Rassi, Yavar

doi:10.4269/ajtmh.2009.08-0469

Cited by 45 publications

(35 citation statements)

References 33 publications

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“…Several epidemiological and entomological findings, including anthropophily and common infection of the sand flies with the same Leishmania parasite that found in patient in the same places, suggested the capacity of sand fly as a vector [17,18] . Recently, molecular techniques (PCR) have been employed for vector incrimination of sand flies [18][19][20] . The highly sensitive technique of PCR has been used for Leishmania in sand flies in some endemic areas, including Iran and India [3,4,18,19,[21][22][23] .…”

Section: Discussionmentioning

confidence: 99%

“…Recently, molecular techniques (PCR) have been employed for vector incrimination of sand flies [18][19][20] . The highly sensitive technique of PCR has been used for Leishmania in sand flies in some endemic areas, including Iran and India [3,4,18,19,[21][22][23] . Results of our study revealed that the high density of P. papatasi in indoor, out door and rodent burrow resting places and its infection with L. major is attributed to the fact that this species plays a major role as a principal vector in the region.…”

Section: Discussionmentioning

confidence: 99%

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First detection of Leishmania infantum DNA in wild caught Phlebotomus papatasi in endemic focus of cutaneous leishmaniasis, South of Iran

Rassi

Hadi

Reza

et al. 2013

Asian Pacific Journal of Tropical Biomedicine

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

First detection of Leishmania infantum DNA in wild caught Phlebotomus papatasi in endemic focus of cutaneous leishmaniasis, South of Iran

Rassi

Hadi

Reza

et al. 2013

Asian Pacific Journal of Tropical Biomedicine

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“…The patients were selected from East Azarbaijan (a city located in northwest of Iran) where the Leishmania infantum is endemic (12-14), while, other species were not reported. Visceral leishmaniasis was diagnosed by an expert specialist according to medical history, laboratory findings, and clinical presentations.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of FcγRIIIB-NA1/NA2 Polymorphism in Visceral Leishmaniasis

Abasi¹,

Lotfi²,

Bazmani³

et al. 2014

Iran Red Crescent Med J

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Background:Several lines of evidence demonstrating that innate and adaptive immunity play important roles in the defense against visceral leishmaniasis (VL). A polymorphism within the FcγRIIIB gene can lead to the expression of three variants of NA1, NA2, and the combined one (NA1/NA2) which alters affinity of IgG to its receptor.Objectives:The main aim of this study was to evaluate the FcγRIIIB-NA1/NA2 polymorphism in the FcγRIIIB gene of VL patients in comparison to healthy controls.Patients and Methods:In this cross-sectional study, three groups; 54 seropositive patients with clinical presentation of VL (group 1), 104 seropositive patients without clinical presentation (group 2), and 104 healthy controls (group 3) were evaluated with respect to the FcγRIIIB-NA1/NA2 polymorphism using a PCR-SSP method. The titration of anti-leishmania antibodies was analyzed using an immunoflorescence technique.Results:Our results indicated that polymorphisms within the FcγRIIIB gene (that lead to the expression of the NA1/NA2 isoforms) are significantly associated with VL. The results demonstrated that the genotype heterozygotic for FcγRIIIB-NA1/NA2 expression was significantly increased in VL patients, group 1 when compared to groups 2 and 3. Conversely, there is a decrease in homozygous NA1 and NA2 genotypes in VL patients; however, the overall frequency of NA1 and NA2 alleles appear similar across the three cohorts examined.Conclusions:According to our results, it is likely that the increased frequency of the FcγRIIIB-NA1/NA2 genotype is associated with impaired immune responses against VL and its subsequent clearance from the patient.

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“…The restriction fragments were subjected to electrophoresis in 3% agarose gel containing ethidium bromide (0.5 μg/ mL) for 3 h at 65 V and observed on a UV transilluminator [20,21].…”

Section: Dna Amplification and Pcr-rflpsmentioning

confidence: 99%

First detection of Leishmania infantum in Phlebotomus kandelakii using molecular methods in north-eastern Islamic Republic of Iran

Rassi¹,

Mr²,

Oshaghi³

et al. 2012

East Mediterr Health J

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Shirvan district in north-eastern Islamic Republic of Iran is a new focus of visceral leishmaniasis. This study aimed to identify the vector(s), the parasite and the species composition of sand flies in the district during JulySeptember 2009 using polymerase chain reaction techniques. In all, 2088 sand flies were collected from 3 villages. Nine sand fly species were identified: Phlebotomus kandelakii (Shchurenkova), P. major (Annandale), P. halepensis (Theodor), P. papatasi (Scopoli), P. sergenti (Parrot), P. longidactus (Parrot), P. caucasicus (Marzinovsky), Sergentomyia sintoni (Pringle) and S. sumbarica (Perfil'ev). P. sergenti and P. kandelakii were the most prevalent Phlebotomus species at 31.3% and 10.0% respectively. Of 59 female P. kandelakii, 2 (3.4%) were naturally infected with L. infantum. This is the first finding of natural infection of P. kandelakii by L. infantum in this region suggesting P. kandelakii may be the vector of L. infantum in the area although it is the second most prevalent phlebotomine species. Perfil'ev). P. sergenti et P. kandelakii étaient les espèces de phlébotomes les plus répandues (31,3 % et 10 % de l'échantillon respectivement). Sur 59 P. kandelakii femelles, deux (3,4 %) étaient naturellement infectées par L. infantum. Il s'agit de la première détection d'une infection naturelle de P. kandelakii par L. infantum dans la région, suggérant que P. kandelakii pourrait être le vecteur de L. infantum dans la zone, même si elle n'est que la deuxième espèce la plus répandue de phlébotomes.

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Vector Incrimination of Sand Flies in the Most Important Visceral Leishmaniasis Focus in Iran

Cited by 45 publications

References 33 publications

First detection of Leishmania infantum DNA in wild caught Phlebotomus papatasi in endemic focus of cutaneous leishmaniasis, South of Iran

First detection of Leishmania infantum DNA in wild caught Phlebotomus papatasi in endemic focus of cutaneous leishmaniasis, South of Iran

Evaluation of FcγRIIIB-NA1/NA2 Polymorphism in Visceral Leishmaniasis

First detection of Leishmania infantum in Phlebotomus kandelakii using molecular methods in north-eastern Islamic Republic of Iran

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