Vector competence of <i>Aedes aegypti, Aedes albopictus</i>, and <i>Culex quinquefasciatus</i> mosquitoes for Mayaro virus

Moreira, Luciano Andrade; Pereira, Thiago Nunes; Carvalho, Fabiano Duarte; Mendonça, Silvana Faria De; Rocha, Marcele Neves

doi:10.1101/661884

Cited by 6 publications

(9 citation statements)

References 46 publications

(39 reference statements)

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“…This connection likely extends to other mosquito-borne viruses such as Mayaro virus and West Nile virus, which have been reported in Haiti [58][59][60] . The former can be transmitted by Aedes mosquitoes 14,[61][62][63] , and the latter has been isolated from zoonotic reservoirs in the Dominican Republic 64 . Similarly, reports of international travelers carrying mosquito-borne viruses from the Dominican Republic back to their home countries demonstrate that gaps in surveillance have global implications [65][66][67][68][69][70] .…”

Section: Discussionmentioning

confidence: 99%

Asynchronicity of endemic and emerging mosquito-borne disease outbreaks in the Dominican Republic

Earnest

Lourenço

et al. 2021

Nat Commun

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Mosquito-borne viruses threaten the Caribbean due to the region’s tropical climate and seasonal reception of international tourists. Outbreaks of chikungunya and Zika have demonstrated the rapidity with which these viruses can spread. Concurrently, dengue fever cases have climbed over the past decade. Sustainable disease control measures are urgently needed to quell virus transmission and prevent future outbreaks. Here, to improve upon current control methods, we analyze temporal and spatial patterns of chikungunya, Zika, and dengue outbreaks reported in the Dominican Republic between 2012 and 2018. The viruses that cause these outbreaks are transmitted by Aedes mosquitoes, which are sensitive to seasonal climatological variability. We evaluate whether climate and the spatio-temporal dynamics of dengue outbreaks could explain patterns of emerging disease outbreaks. We find that emerging disease outbreaks were robust to the climatological and spatio-temporal constraints defining seasonal dengue outbreak dynamics, indicating that constant surveillance is required to prevent future health crises.

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Section: Discussionmentioning

confidence: 99%

Asynchronicity of endemic and emerging mosquito-borne disease outbreaks in the Dominican Republic

Earnest

Lourenço

et al. 2021

Nat Commun

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“…This connection likely extends to other mosquito-borne viruses such as Mayaro virus and West Nile virus, which have been reported in Haiti (Blohm et al, 2019; Lednicky et al, 2016; Weppelmann et al, 2017). The former can be transmitted by Aedes mosquitoes (Kantor et al, 2019; Long et al, 2011; Pereira et al, 2020; Wiggins et al, 2018), and the latter has been isolated from zoonotic reservoirs in the Dominican Republic (Komar et al, 2005). Similarly, reports of international travelers carrying mosquito-borne viruses from the Dominican Republic back to their home countries demonstrate that gaps in surveillance have global implications (Barzon et al, 2016; Díaz-Menéndez et al, 2018; Duijster et al, 2016; Goncé et al, 2018; Millman et al, 2016; Requena-Méndez et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Asynchronicity of endemic and emerging mosquito-borne disease outbreaks in the Dominican Republic

Petrone

Earnest

Lourenço

et al. 2020

Preprint

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Mosquito-borne viruses pose a perpetual public health threat to countries and territories in the Carribean due to the region's tropical climate and seasonal reception of international tourists. Outbreaks of the emerging viruses chikungunya and Zika in 2014 and 2016, respectively, demonstrated the rapidity with which these viruses can spread between islands. At the same time, the number of reported dengue fever cases, caused by the endemic dengue virus, has steadily climbed over the past decade, and a large dengue outbreak that began sweeping through this region in 2019 continues in 2020. Sustainable disease and mosquito control measures are urgently needed to quell virus transmission in the long term and prevent future outbreaks from occurring. To improve upon current surveillance methods, we analyzed temporal and spatial patterns of chikungunya, Zika, and dengue outbreaks reported in the Dominican Republic between 2012 and 2018. The viruses that caused these outbreaks are transmitted by Aedes mosquitoes, which are sensitive to seasonal climatological variability. In this study, we evaluated whether climate and the spatio-temporal dynamics of past dengue outbreaks could inform when and where future emerging disease outbreaks might occur. We found that the temporal and spatial distribution of emerging disease outbreaks did not conform to those of seasonal dengue outbreaks. Rather, the former occurred when climatological conditions were suboptimal for Aedes activity. Provincial dengue attack rates did not correspond to those of emerging diseases. Our study also provides evidence for under-reporting of dengue cases, especially following the 2016 Zika outbreak. We advocate for the implementation of a sustainable and long-term surveillance system to monitor the spread of known mosquito-borne viruses and to identify emerging threats before they cause outbreaks. Specifically, we recommend the use of febrile illness incidence, case fatality rates, and serosurveys during inter-outbreak periods to better understand rates of transmission and asymptomatic infection.

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“…To test CS technique for infection, we used Mayaro virus (MAYV). The MAYV vector competence has been previously tested by our group for these mosquito species (Pereira et al, 2020). MAYV was grown in Ae.…”

Section: Mosquito Infectionmentioning

confidence: 99%

“…These mosquitoes require blood feeding for obtaining proteins and egg maturation (Forattini 2002;Farnesi et al, 2018). In general, the blood feeding activity of arthropods can cause problems to humans (Ajelli, 2017;Bergquist et al, 2017;Benelli e Duggan, 2018), since it is during the hematophagy process that pathogens causing malaria, leishmaniasis, filariasis, dengue, zika, chikungunya, yellow fever and mayaro are transmitted (Gluber, 1998;Lehane, 2005, Cohnstaedt et al, 2017Medeiros et al, 2019;Pereira et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Simplified artificial blood feeding and oral infection method for mosquitoes

Pereira

Carvalho

Silva

et al. 2020

Preprint

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Mosquitoes such as Aedes aegypti, Aedes albopictus and Culex quinquefasciatus are vectors of many pathogens that greatly affect humankind. The maintenance of these mosquitoes in laboratory permit different studies that can help understanding their biology, as well as the vector-pathogen interaction. In addition to sugar meals, the blood feeding is essential for maintenance of the reproductive cycle in several vectors. The main blood sources for many mosquito colonies are direct feeding on live animal or the use of human/animal blood through artificial feeders. However, this latter process has some disadvantages, as artificial feeders can be very laborious for assembly and decontamination. Based on these observations, a simplified technique for feeding and artificial infection was developed with cotton-pads soaked (CS) and blood or blood and viral supernatant to simulate an artificial infection. The efficiency of the CS technique was investigated through the number of mosquitoes fed/infected, when compared to their respective control group. The CS technique, with blood at room temperature, promoted a feeding rate of 61.4% for Ae. albopictus, 70.8% for Cx. quinquefasciatus and 17% for Ae. aegypti. The control group (Hemotec-feeding) presented 47.9%, 16.5% and 59.1% of feeding success, respectively. To improve the CS technique for Ae. aegypti mosquitoes, the procedure was then performed with blood at 38°C, which was possible to observe a feeding rate of 47.3%, in comparison to 53.2% for the control group (Hemotec). When using the CS technique for artificial infection with Mayaro virus, more than 80% of infection was observed for Ae. aegypti and 100% for Ae. albopictus. In the traditional infection technique (glass feeder), the infection rate was 90% (Ae. aegypti) and 96.6% (Ae. albopictus). For Cx. quinquefasciatus, the infection was positive only with the CS technique, resulting in 1 (5%) mosquito infected with Mayaro virus. Our results suggest that this simplified technique of low-cost feeding and easy assembly, offers good results for feeding (maintenance of colonies) and artificial infection of different species of mosquitoes.

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Vector competence of Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus mosquitoes for Mayaro virus

Cited by 6 publications

References 46 publications

Asynchronicity of endemic and emerging mosquito-borne disease outbreaks in the Dominican Republic

Asynchronicity of endemic and emerging mosquito-borne disease outbreaks in the Dominican Republic

Asynchronicity of endemic and emerging mosquito-borne disease outbreaks in the Dominican Republic

Simplified artificial blood feeding and oral infection method for mosquitoes

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