VDJ recombination

Alt, Frederick W.; Oltz, Eugene M.; Young, Faith; Gorman, James R.; Taccioli, Guillermo E.; Chen, Jianzhu

doi:10.1016/0167-5699(92)90043-7

Cited by 326 publications

(206 citation statements)

References 74 publications

Supporting

Mentioning

195

Contrasting

Unclassified

Order By: Relevance

“…Our results show that the transcription of Rag1 and Rag2 were up-regulated in zebrafish exposed to 800 µg/L MC-LR. Since the RAG genes are required by maturing B-and T-cells, essential for antigen receptor gene rearrangement by somatic recombination (Alt et al, 1992), their increased transcription may accelerate the maturation of B-and T-cells, which in turn may produce some specific molecules to inhibit the toxic effects of MC-LR.…”

Section: Discussionmentioning

confidence: 99%

Effects of pure microcystin-LR on the transcription of immune related genes and heat shock proteins in larval stage of zebrafish (Danio rerio)

Sun

et al. 2009

Aquaculture

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Effects of pure microcystin-LR on the transcription of immune related genes and heat shock proteins in larval stage of zebrafish (Danio rerio)

Sun

et al. 2009

Aquaculture

View full text Add to dashboard Cite

“…The repair of DNA double-strand breaks (DSBs) is required for cellular survival after exposure to ionizing radiation as well as the normal rearrangement of immunoglobulin and T cell receptor genes in lymphocytes (1)(2)(3). Therefore, DSB repair is critical to normal development of the immune system and the maintenance of genomic integrity.…”

mentioning

confidence: 99%

DNA looping by Ku and the DNA-dependent protein kinase

Cary

Peterson

Wang

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

228

138

View full text Add to dashboard Cite

The DNA-dependent protein kinase (DNA-PK) is required for DNA double-strand break (DSB) repair and immunoglobulin gene rearrangement and may play a role in the regulation of transcription. The DNA-PK holoenzyme is composed of three polypeptide subunits: the DNA binding Ku70͞86 heterodimer and an Ϸ460-kDa catalytic subunit (DNA-PKcs). DNA-PK has been hypothesized to assemble at DNA DSBs and play structural as well as signal transduction roles in DSB repair. Recent advances in atomic force microscopy (AFM) have resulted in a technology capable of producing high resolution images of native protein and proteinnucleic acid complexes without staining or metal coating. The AFM provides a rapid and direct means of probing the protein-nucleic acid interactions responsible for DNA repair and genetic regulation. Here we have employed AFM as well as electron microscopy to visualize Ku and DNA-PK in association with DNA. A significant number of DNA molecules formed loops in the presence of Ku. DNA looping appeared to be sequence-independent and unaffected by the presence of DNA-PKcs. Gel filtration of Ku in the absence and the presence of DNA indicates that Ku does not form nonspecific aggregates. We conclude that, when bound to DNA, Ku is capable of self-association. These findings suggest that Ku binding at DNA DSBs will result in Ku self-association and a physical tethering of the broken DNA strands.

show abstract

“…[1][2][3][4][5][6] Signals in site-specific pathways define the points at which the exchange will occur. General signals do not promote defined rearrangements but rather create hotspots by increasing the frequency of homologous recombination in their vicinity.…”

Section: Introductionmentioning

confidence: 99%

Capture of a recombination activating sequence from mammalian cells

Olson

Dornburg

1999

Gene Ther

View full text Add to dashboard Cite

We have developed a genetic trap for identifying revealed a putative recombination signal (CCCACCC). sequences that promote homologous DNA recombination.When this heptamer or an abbreviated form (CCCACC) The trap employs a retroviral vector that normally disables were reinserted into the vector, they stimulated vector itself after one round of replication. Insertion of defined repair and other DNA rearrangements. Mutant forms of DNA sequences into the vector induced the repair of a 300 these oligomers (eg CCCAACC or CCWACWS) did not. base pair deletion, which restored its ability to replicate.Our data suggest that the recombination events occurred Tests of random sequence libraries made in the vector within 48 h after transfection.

show abstract

VDJ recombination

Cited by 326 publications

References 74 publications

Effects of pure microcystin-LR on the transcription of immune related genes and heat shock proteins in larval stage of zebrafish (Danio rerio)

Effects of pure microcystin-LR on the transcription of immune related genes and heat shock proteins in larval stage of zebrafish (Danio rerio)

DNA looping by Ku and the DNA-dependent protein kinase

Capture of a recombination activating sequence from mammalian cells

Contact Info

Product

Resources

About