Vaspin mRNA levels in the liver of morbidly obese women with nonalcoholic fatty liver disease

Waluga, Marek; Kukla, Michał; Żorniak, Michał; Grabiec, Marta; Kajor, Maciej; Dyaczyński, Michał; Kowalski, Greg M.; Żądło, Dominika; Berdowska, Agnieszka; Kotulski, Rafał; Bułdak, Rafał Jakub; Sawczyn, Tomasz; Waluga, Ewa; Olczyk, Paweł; Hartleb, Marek

doi:10.5114/pjp.2017.69688

Cited by 4 publications

(4 citation statements)

References 21 publications

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“…The difference between our results and those of the aforementioned study could be due to differences in the clinical characteristics of included participants (T2DM patients versus mixed population), ethnicity, genetics, and dietary practices, especially as dietary intake and composition have been shown to play a considerable role in the development of NAFLD as mentioned earlier [41], and there have been reports of ethnicity-associated differences in the pathogenesis and development of NAFLD [43]. Furthermore, a study among morbidly obese females undergoing bariatric surgery did not show any association between pathological features of NASH and plasma FGF-21, which suggests that body fat and gender, as well as other comorbidities, may modify this association [44].…”

Section: Discussionmentioning

confidence: 83%

Fibroblast Growth Factor 21: A More Effective Biomarker Than Free Fatty Acids and Other Insulin Sensitivity Measures for Predicting Non-alcoholic Fatty Liver Disease in Saudi Arabian Type 2 Diabetes Patients

Bahijri,

Eldakhakhny,

Enani

et al. 2023

Cureus

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BackgroundNon-alcoholic fatty liver disease (NAFLD) is more prevalent among individuals with type 2 diabetes (T2DM), elevating their risk of cardiovascular diseases (CVDs) and premature mortality. There is a need to modify treatment strategies to prevent or delay these adverse outcomes. Currently, there are no sensitive or specific biomarkers for predicting NAFLD in Saudi T2DM patients. Therefore, we aimed to explore the possibility of using fibroblast growth factor 21 (FGF-21), free fatty acids (FFAs), homeostatic model assessment for insulin resistance (HOMA-IR), and quantitative insulin sensitivity check index (QUICKI) as possible markers. MethodologyIn this study, a total of 67 T2DM patients were recruited. NAFLD was detected by ultrasonography in 28 patients. Plasma glucose, FFAs, FGF-21, and serum insulin were measured in fasting blood samples. HOMA-IR and QUICKI were calculated. The means of the two groups with and without NAFLD were statistically compared. The receiver operating characteristics (ROC) curve and the area under the curve (AUC) were used to assess the ability to identify NAFLD. ResultsThe mean levels of FGF-21 and HOMA-IR were significantly higher and that of QUICKI was significantly lower in patients with NAFLD than in those without (p < 0.001, p = 0.023, and p = 0.018, respectively). FGF-21 had the highest AUC to identify NAFLD (AUC = 0.981, 95% confidence interval = 0.954-1, P < 0.001). The AUCs for HOMA-IR, QUICKI, and FFA were <0.7. The highest sensitivity, specificity, positive likelihood ratio, and the lowest negative likelihood ratio were found when FGF-21 was used to predict NAFLD. ConclusionsFGF-21 may be used as a biomarker to predict NAFLD in people with T2DM due to its high sensitivity and specificity compared to the other markers.

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Section: Discussionmentioning

confidence: 83%

Fibroblast Growth Factor 21: A More Effective Biomarker Than Free Fatty Acids and Other Insulin Sensitivity Measures for Predicting Non-alcoholic Fatty Liver Disease in Saudi Arabian Type 2 Diabetes Patients

Bahijri,

Eldakhakhny,

Enani

et al. 2023

Cureus

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“…The main risk factor of HCC in chronic liver diseases is advanced fibrosis/cirrhosis, which is strictly associated with angiogenesis intensity [7, 34]. Vaspin serum levels and mRNA liver expression were found to be increased in patients with advanced fibrosis or cirrhosis in the course of chronic hepatitis [35, 36]. Pointing to these results, vaspin plays pivotal role in HCC development.…”

Section: Discussionmentioning

confidence: 99%

The adipokine vaspin reduces apoptosis in human hepatocellular carcinoma (Hep-3B) cells, associated with lower levels of NO and superoxide anion

Skonieczna

Hudy

Hejmo

et al. 2019

BMC Pharmacol Toxicol

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Background Among adipose-derived factors, adipocytokines play roles as hormones and signaling mediators for apoptotic pathway. Among of them, vaspin, regulates the metabolism of adipose tissue itself as an endocrine organ, and stimulates adipocytes to maturation, differentiation, etc. Damaged adipocytes, present in obesity and hepatocellular carcinoma (HCC) respond with over-production of inflammatory cytokines. Such pro-inflammatory stimulation remains under adipokine control. Pro-inflammatory pathways are connected to oxidative stress and apoptosis, reported as co-existing with an elevated level of some adipokines in cancer cell lines. However, some hormones, such as vaspin, reduce apoptosis, have anti-inflammatory and anti-oxidative roles in cancer cell lines. Methods Hep-3B cells were cytometrically evaluated under vaspin treatment for reactive oxygen species (ROS) and apoptosiss induction. The statistical significant changes to the untreated controls was calculated by T-tests (indicated at value p < 0.05). Results Here we studied the production of reactive oxygen and nitrogen species in cells of HCC line Hep-3B after vaspin treatment. A decreased level of nitric oxide and superoxide anion 24 h after vaspin addition at 5 ng/ml was correlated with restricted, to the physiological level, apoptosis. A protective role of vaspin was displayed as enhanced cell viability and proliferation, which could be a poor prognostic in liver cancer. Conclusions Apoptosis was suppressed after vaspin treatment, together with low levels of nitric oxide and superoxide anions.

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“…Regarding the articles with a low score (<5), the studies conducted by Liu et al and Wang (36) did not include community controls and lacked specific selection criteria for healthy controls (31,36). The studies by Waluga et al also did not include community controls and failed to meet the standard of representativeness (34). According to the GRADE scale (https:// gdt.gradepro.org), the certainty of the present meta-analysis was very low (see Supplementary Table 2).…”

Section: Quality Assessmentmentioning

confidence: 99%

“…This meta-analysis examined 12 studies consisting of 14 comparisons of omentin levels between MAFLD and control groups (Figure 2) (28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39). Among these, the articles by Li et abdominal obesity).…”

Section: Association Between Circulating Omentin Levels and Mafldmentioning

confidence: 99%

Association of circulating omentin level and metabolic-associated fatty liver disease: a systematic review and meta-analysis

Zhang

Chen

et al. 2023

Front. Endocrinol.

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BackgroundMetabolic-associated fatty liver disease (MAFLD) is closely associated with omentin, a novel adipokine that plays a vital role in metabolic balance. The literature about the relationship between circulating omentin and MAFLD is conflicting. Therefore, this meta-analysis evaluated circulating omentin levels in patients with MAFLD compared with healthy controls to explore the role of omentin in MAFLD.MethodsThe literature search was performed up to April 8, 2022, using PubMed, Cochrane Library, EMBASE, CNKI, Wanfang, CBM, Clinical Trials Database and Grey Literature Database. This meta-analysis pooled the statistics in Stata and presented the overall results using the standardized mean difference (SMD) and 95% confidence interval (CI).ResultsTwelve studies with 1624 individuals (927 cases and 697 controls) were included, and all of them were case-control studies. In addition, ten of twelve included studies were conducted on Asian participants. Patients with MAFLD had significantly lower circulating omentin levels than healthy controls (SMD=-0.950 [-1.724, -0.177], P=0.016). Subgroup analysis and meta-regression demonstrated that fasting blood glucose (FBG) might be the source of heterogeneity and was inversely associated with omentin levels (coefficient=-0.538, P=0.009). No significant publication bias existed (P>0.05), and outcomes were robust in the sensitivity analysis.ConclusionLower circulating omentin levels were associated with MAFLD, and FBG might be the source of heterogeneity. Since Asian studies accounted for a significant portion of the meta-analysis, the conclusion might be more applicable to the Asian population. By investigating the relationship between omentin and MAFLD, this meta-analysis laid the foundation for the development of diagnostic biomarkers and treatment targets.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, identifier CRD42022316369.

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Vaspin mRNA levels in the liver of morbidly obese women with nonalcoholic fatty liver disease

Cited by 4 publications

References 21 publications

Fibroblast Growth Factor 21: A More Effective Biomarker Than Free Fatty Acids and Other Insulin Sensitivity Measures for Predicting Non-alcoholic Fatty Liver Disease in Saudi Arabian Type 2 Diabetes Patients

Fibroblast Growth Factor 21: A More Effective Biomarker Than Free Fatty Acids and Other Insulin Sensitivity Measures for Predicting Non-alcoholic Fatty Liver Disease in Saudi Arabian Type 2 Diabetes Patients

The adipokine vaspin reduces apoptosis in human hepatocellular carcinoma (Hep-3B) cells, associated with lower levels of NO and superoxide anion

Association of circulating omentin level and metabolic-associated fatty liver disease: a systematic review and meta-analysis

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