Vasorelaxing effect of Garcinia cowa leaf extract in rat thoracic aorta and its underlying mechanisms

Yorsin, Somruedee; Sriwiriyajan, Somchai; Chongsa, Watchara

doi:10.1016/j.jtcme.2022.12.001

Cited by 6 publications

(2 citation statements)

References 32 publications

(31 reference statements)

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“…After the final NA-induced contraction, the extract was added in a volume of 100 µL; then, concentration–response assays were conducted for each arterial ring, the relaxation was compared before and after the addition of extracts, using Acknowledge 3.8.1 software (Biopac ® ) [ 17 ]. To evaluate the vasorelaxant action due to PP Am , two approaches were followed: in endothelium containing aortic rings, enzyme inhibitors of endothelium signaling pathways were used, as follows: 15 min before last NA-induced contraction, aortic rings were exposed to L-NAME 175 (10 μM, nitric oxide synthases inhibitor), or indomethacin (10 μM, cyclooxygenases inhibitor), accordingly [ 18 , 19 ]. Then, concentration–response curves of extract-induced relaxation were obtained.…”

Section: Methodsmentioning

confidence: 99%

LC-MS Fingerprinting Development for Standardized Precipitate from Agastache mexicana, Which Induces Antihypertensive Effect through NO Production and Calcium Channel Blockade

Cruz-Torres,

Estrada-Soto,

Arias-Durán

et al. 2023

Pharmaceutics

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The aim of this work was to evaluate the vasorelaxant and antihypertensive effects of a standardized precipitate of the hydroalcoholic extract from Agastache mexicana (PPAm), comprising ursolic acid, oleanolic acid, acacetin, luteolin and tilianin, among others. In the ex vivo experiments, preincubation with L-NAME (nonspecific inhibitor of nitric oxide synthases) reduced the relaxation induced by PPAm; nevertheless, preincubation with indomethacin (nonspecific inhibitor of cyclooxygenases) did not generate any change in the vasorelaxation, and an opposed effect was observed to the contraction generated by CaCl2 addition. Oral administration of 100 mg/kg of PPAm induced a significant acute decrease in diastolic (DBP) and systolic (SBP) blood pressure in spontaneously hypertensive rats, without changes in heart rate. Additionally, PPAm showed a sustained antihypertensive subacute effect on both DBP and SBP for 10 days compared to the control group. On the other hand, human umbilical vein cells treated with 10 µg/mL of PPAm showed a significant reduction (p < 0.05) in intracellular adhesion molecule-1, compared to the control, but not on vascular cell adhesion molecule-1. In conclusion, PPAm induces a significant antihypertensive effect in acute- and subacute-period treatments, due to its direct vasorelaxant action on rat aortic rings through NO production and Ca2+ channel blockade.

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Section: Methodsmentioning

confidence: 99%

LC-MS Fingerprinting Development for Standardized Precipitate from Agastache mexicana, Which Induces Antihypertensive Effect through NO Production and Calcium Channel Blockade

Cruz-Torres,

Estrada-Soto,

Arias-Durán

et al. 2023

Pharmaceutics

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“…For instance, the flavone luteolin, elicited its antihypertensive effect through the activation of the cAMP/protein kinase A signaling pathway, hence inducing the activation of NO synthase (Loh et al, 2020). Similarly, both quercetin and kaempferol induced a potent vasodilatory effect that was partially mediated by endothelium‐derived NO (Yorsin et al, 2023). Also, epicatechin induced an antihypertensive effect is mediated by increased activity of NO‐synthase (Kluknavsky et al, 2016).…”

Section: Flavonoids and Vascular Tonementioning

confidence: 99%

Current insights and future perspectives of flavonoids: A promising antihypertensive approach

Harahap,

Syahputra,

Ahmed

et al. 2024

Phytotherapy Research

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Hypertension, or high blood pressure (BP), is a complex disease influenced by various risk factors. It is characterized by persistent elevation of BP levels, typically exceeding 140/90 mmHg. Endothelial dysfunction and reduced nitric oxide (NO) bioavailability play crucial roles in hypertension development. L‐NG‐nitro arginine methyl ester (L‐NAME), an analog of L‐arginine, inhibits endothelial NO synthase (eNOS) enzymes, leading to decreased NO production and increased BP. Animal models exposed to L‐NAME manifest hypertension, making it a useful design for studying the hypertension condition. Natural products have gained interest as alternative approaches for managing hypertension. Flavonoids, abundant in fruits, vegetables, and other plant sources, have potential cardiovascular benefits, including antihypertensive effects. Flavonoids have been extensively studied in cell cultures, animal models, and, to lesser extent, in human trials to evaluate their effectiveness against L‐NAME‐induced hypertension. This comprehensive review summarizes the antihypertensive activity of specific flavonoids, including quercetin, luteolin, rutin, troxerutin, apigenin, and chrysin, in L‐NAME‐induced hypertension models. Flavonoids possess antioxidant properties that mitigate oxidative stress, a major contributor to endothelial dysfunction and hypertension. They enhance endothelial function by promoting NO bioavailability, vasodilation, and the preservation of vascular homeostasis. Flavonoids also modulate vasoactive factors involved in BP regulation, such as angiotensin‐converting enzyme (ACE) and endothelin‐1. Moreover, they exhibit anti‐inflammatory effects, attenuating inflammation‐mediated hypertension. This review provides compelling evidence for the antihypertensive potential of flavonoids against L‐NAME‐induced hypertension. Their multifaceted mechanisms of action suggest their ability to target multiple pathways involved in hypertension development. Nonetheless, the reviewed studies contribute to the evidence supporting the useful of flavonoids for hypertension prevention and treatment. In conclusion, flavonoids represent a promising class of natural compounds for combating hypertension. This comprehensive review serves as a valuable resource summarizing the current knowledge on the antihypertensive effects of specific flavonoids, facilitating further investigation and guiding the development of novel therapeutic strategies for hypertension management.

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Exploring the diverse biological activities of Garcinia cowa: Implications for future cancer chemotherapy and beyond

Rahman,

Panichayupakaranant

2024

Food Bioscience

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Vasorelaxing effect of Garcinia cowa leaf extract in rat thoracic aorta and its underlying mechanisms

Cited by 6 publications

References 32 publications

LC-MS Fingerprinting Development for Standardized Precipitate from Agastache mexicana, Which Induces Antihypertensive Effect through NO Production and Calcium Channel Blockade

LC-MS Fingerprinting Development for Standardized Precipitate from Agastache mexicana, Which Induces Antihypertensive Effect through NO Production and Calcium Channel Blockade

Current insights and future perspectives of flavonoids: A promising antihypertensive approach

Exploring the diverse biological activities of Garcinia cowa: Implications for future cancer chemotherapy and beyond

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