Vasorelaxation Effects of 2-Chloroethanol and Chloroacetaldehyde in the Isolated Rat Aortic Rings

Abstract

2-Chloroethanol (2-CE) is a commonly used solvent in industry; unfortunately, severe hypotension is one of main toxic signs during intoxication. Calcium ion modulation is considered to be an important role of vasorelaxation. The aim of this study is to evaluate either 2-CE or its main metabolite, chloroacetaldehyde (CAA), possible cause of hypotension, by using isolated rat aortic rings. Results revealed that 2-CE caused a weakly relaxation in the phenylephrine (PE) pre-induced endothelium-intact aortic rings. However, its metabolite, CAA induced vasorelaxation and showed dose dependency in endothelium-intact and -denuded aortic rings. The half inhibitory concentration (IC 50 ) of 2-CE exceeded 50 mM; meanwhile, the IC 50 values of CAA in the endothelium-intact and -denuded aortic rings were 3.3 and 2.7 mM, respectively. The CAA-induced relaxation could be significantly attenuated by adding calcium (CaCl 2 ) and various Ca 2+ channel blockers, dantrolene, nifedipine, and NiCl 2 . Nifedipine presents the most strong inhibition effect among the calcium blockers. In conclusion, it is suggested that the hypotension effect of 2-CE intoxicated cases may be mainly mediated by its metabolite CAA, and calcium channels are partially involved inducing the vasorelaxation.

In2O3 microbundles constructed with well-aligned single-crystalline nanorods: F127-directed self-assembly and enhanced gas sensing performance

“…2-Chloroethanol, a typical VOC, is widely used in the manufacture pesticides, plasticizers, plant-protection agents, and dye intermediates. According to the recent reports, excess intake of 2-chloroethanol will lead to hypotension, metabolic acidosis, coma, respiratory failure, and death [45]. As a result, the admissible concentration of 2-chloroethanol is strictly limited in workplace.…”

Effects of Acrylamide-Induced Vasorelaxation and Neuromuscular Blockage: A Rodent Study

“…The rat aortic rings were isolated according to previously described methods [ 28 ]. The rats were anaesthetised with an intraperitoneal injection of urethane (0.6 g/kg body weight) combined with chlorohydrate (0.4 g/kg body weight).…”

“…Aortic ring contraction was recorded using a force-displacement transducer (Grass SD9 stimulator; Quincy, MA, USA) that was connected to a PowerLab recorder (ADIntruments, New South Wales, Australia). The aorta rings were denuded and the endothelium was removed by rubbing with cotton; the absence of ACh-induced relaxation indicated successful denudation [ 28 ].…”

“…Two "L"-type stainless steel hooks were then inse one fixed to the bottom and the other connected to a fo were maintained under the optimal tension of 1 g for 45 traction was recorded using a force-displacement tran Quincy, MA, USA) that was connected to a PowerLab South Wales, Australia). The aorta rings were denuded an by rubbing with cotton; the absence of ACh-induced rela udation [28].…”