Vasopressin induces endolymphatic hydrops in mouse inner ear, as evaluated with repeated 9.4 T MRI

Endolymphatic hydrops induced by different mechanisms responds differentially to spironolactone: a rationale for understanding the diversity of treatment responses in hydropic inner ear disease

“…We have previously shown that chronic administration of vasopressin and inhibitors of the cAMP/cGMP degrading enzymes PDE3, PDE4 and PDE5 to mice results in the development of EH [10,11]. In the present study, we show that spironolactone, an aldosterone and mineralocorticoid receptor antagonist, prevents hydrops induced by vasopressin and the PDE4 inhibitor rolipram, but not by the PDE3 inhibitor cilostamide.…”

“…The pumps were implanted surgically in the subcutaneous tissues on the back between the scapulae. Gadolinium contrast agent (Dotarem, Guerbet, Villepinte, France) (gadoteric acid; 279.3 mg/ml, 0.5 mmol/ml) was administered intraperitoneally (100 ll/20g) in the left abdominal quadrant [10,11]. 55-65 min after Gadolinium administration, contrast enhanced MR images were obtained at day zero and 4 weeks after initiation of hormone/drug administration.…”

“…MR imaging was performed as previously described [10,11] with a 9.4 T magnet (Agilent Inc., Palo Alto, CA) using Avance III electronics (Bruker, Ettlingen, Germany). The system is equipped with a 12 cm inner diameter gradient system having a maximum gradient strength of 670 mT/m.…”

Claudin expression in the rat endolymphatic duct and sac - first insights into regulation of the paracellular barrier by vasopressin

“…We showed that the translocation of Claudin 4 from the cytoplasm into the basolateral membranes and TJ of the epithelia cells was induced in ES specimens stimulated with the AVP analogon dDAVP. In a variety of studies abnormally high plasma levels of AVP were correlated with Menière’s disease32518889. Menière’s disease is a common clinical syndrome of recurrent attacks of vertigo, fluctuating hearing loss and tinnitus9091.…”

Histopathology of Meniere’s Disease

“…16 The rate of endolymph secretion seems to be influenced by a number of factors and hormones, including aldosterone 17 and vasopressin 18 ; other substances such as cathecholamines, thyroid hormones, and somatostatin are also implicated in endolymph homeostasis. 13 But the K+ in endolymph is absorbed by the sensory hair cells via apical transduction channels, from which it is transported back to perilymph.…”