Vasopressin and oxytocin gene expression in intact rats and under catecholamine deficiency during ontogenesis

Trembleau, Alain; Ugrumov, M. V.; Roche, D.; Calas, A.

doi:10.1016/0361-9230(95)00020-f

Cited by 23 publications

(29 citation statements)

References 32 publications

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“…In rats, starting from E18, in situ hybridization studies localized OT neurons in the dorsal portion of the supraoptic nucleus of the hypothalamus, whereas its ventricular portion is occupied by arginine vasopressin neurons. Fewer OT-labeled cells were found in the developing paraventricular nucleus (22). OT-specific binding sites were detected first in the brain at E14 and before OT expression (23).…”

Section: Discussionmentioning

confidence: 91%

Oxytocin in cardiac ontogeny

Jankowski

Danalache²,

Wang³

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

102

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Previous studies demonstrated the presence of oxytocin (OT) and oxytocin receptors (OTRs) in the heart. The present work provides results supporting a potential role of OT in cardiomyogenesis. Here, we show a maximal OT and OTR protein level in the developing rat heart at day 21 of gestation and postnatal days 1-4, when cardiac myocytes are at a stage of intense hyperplasia. Between postnatal days 1 and 66, OT decreased linearly in all heart chambers (4.1-to 6.6-fold). Correspondingly, immunocytochemistry demonstrated that OTRs, which were eminent in postnatal cardiomyocytes, declined with age to low levels in adults. Interestingly, in coronary vasculature, OTRs developed in endothelial cells at postnatal days 12 and 22 and achieved a plateau in adult rats. These findings suggest that OT can be involved in developmental formation of the coronary vessels. In vivo, the OT͞OTR system in the fetal heart was sensitive to the actions of retinoic acid (RA), recognized as a major cardiac morphogen. RA treatment produced a significant increase (2-to 3-fold) both in the OT concentration and in the OT mRNA levels. Ex vivo, an OT antagonist inhibited RA-mediated cardiomyocyte differentiation of P19 embryonic stem cells. The decline of cardiac OT expression from infancy to adulthood of the rat and changes in cell types expressing OTR indicate a dynamic regulation of the OT system in the heart rather than constitutive expression. The results support the hypothesis that RA induces cardiomyogenesis by activation of the cardiac OT system. heart development ͉ oxytocin receptors ͉ retinoic acid ͉ oxytocin antagonist ͉ cardiomyocyte differentiation O xytocin (OT), recognized traditionally as a reproductive hormone with a major role in childbirth and lactation, is produced in high concentrations in the hypothalamic supraoptic nucleus and paraventricular nucleus, then transported from these source nuclei to the posterior pituitary by neurosecretion (1). Longitudinal studies of neural and hormonal circuits activated by experimental volume expansion have identified OT as a major regulator of cardiovascular functions (reviewed in refs. 2 and 3). OT, injected peripherally, causes a decrease of arterial pressure (4) while reducing both heart rate and the force of contractions in isolated atria (5). OT acts via neuroendocrine͞ endocrine͞paracrine pathways to release atrial natriuretic peptide (ANP) from the heart (6, 7). ANP is a potent diuretic, natriuretic, and vasorelaxant hormone that is also involved in cell growth regulation. In addition, we have demonstrated that in isolated, perfused hearts, an OT antagonist (OTA) blocks basal ANP release (7), suggesting the presence of local OT in the heart. Further study revealed cardiac OT synthesis (8), with OT being detected in the medium of cultured neonatal rat cardiomyocytes (9).Recently, we showed that P19 embryonic carcinoma cells, a model of mouse embryonic stem cells, express OT receptors (OTRs), and OT stimulates the differentiation of these cells into beating cell colonies expressing ...

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Section: Discussionmentioning

confidence: 91%

Oxytocin in cardiac ontogeny

Jankowski

Danalache²,

Wang³

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

102

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“…3 H]thymidine autoradiographic, immunocytochemical, and in situ hybridization data Bayer, 1978a,b, 1986;Buijs, 1992;Trembleau et al, 1995), this suggests that these are magnocellular vasopressin and oxytocin neurons that originated from the neuroepithelium of the diamond-like swelling of the third ventricle in rats at E12-E14 followed by their migration first to the SO and then to the PV. Although the DiI labeling of glial cells abutting the meninges due to DiI diffusion also cannot be excluded, the labeled cells may represent a minor cell population compared with the DiI-labeled magnocellular neurons.…”

Section: Magnocellular Nucleimentioning

confidence: 83%

“…These neurons occupy the supraoptic nucleus (SO), accessory nuclei, and the paraventricular nucleus (PV), showing a mediolateral gradient in their final position (Whitnall et al, 1985;Laurent et al, 1989;Buijs, 1992;Trembleau et al, 1995;Rabhi et al, 1996). The development of the oxytocin system appears to be slightly slower than that of the vasopressin system (Whitnall et al, 1985;Boer, 1987;Buijs, 1992).…”

mentioning

confidence: 95%

“…According to [ 3 H]thymidine autoradiography, immunocytochemistry, and in situ hybridization, vasopressin and oxytocin neurons are generated from the neuroepithelium of the diamond-like swelling of the third ventricle at the level of the sulcus ventralis thalami long before birth (Altman and Bayer, 1978a,b;Okamura et al, 1983;Whitnall et al, 1985;Trembleau et al, 1995). These neurons occupy the supraoptic nucleus (SO), accessory nuclei, and the paraventricular nucleus (PV), showing a mediolateral gradient in their final position (Whitnall et al, 1985;Laurent et al, 1989;Buijs, 1992;Trembleau et al, 1995;Rabhi et al, 1996).…”

mentioning

confidence: 99%

“…Over the last two decades, particular attention has been paid to the development of the hypothalamic magnocellular vasopressin and oxytocin systems as well as the functional significance of vasopressin and oxytocin in the developing organism (Choy and Watkins, 1979;Buijs et al, 1980;Whitnall et al, 1985;Boer, 1987;Buijs, 1992;Trembleau et al, 1995). The role of these neurohormones appears to be related to maintenance of homeostasis even before the birth (Ervin et al, 1986;Uhl, 1987, 1988) as well as the control of morphogenesis of target organs (brain, pituitary, kidney, etc.…”

mentioning

confidence: 99%

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Projections from the hypothalamus to the posterior lobe in rats during ontogenesis: 1,1?-dioctadecyl-3,3,3?,3?-tetramethylindocarbocyanine perchlorate tracing study

et al. 2000

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The objective of this study was to determine the schedule of the arrival of the axons from the hypothalamus to the posterior lobe of the pituitary (PL) in rats during ontogenesis by using the fluorescent lipophilic carbocyanine dye 1,1'-dioctadecyl-3,3,3', 3'-tetramethylindocarbocyanine perchlorate (DiI) as a retrograde tracer. After preliminary fixation of the brain, DiI crystals were implanted in the PL on embryonic day 15 (E15), E16, E17, and E19 as well as on postnatal day 2 (P2) and P9. This was followed by a DiI retrograde diffusion along the plasma membrane and subsequent staining of hypothalamic neuronal cell bodies. The supraoptic nucleus (SO) contained an accumulation of fluorescent cells that extended toward the diamond-like swelling of the third ventricle as early as E15. These data suggest that the magnocellular neurons of the SO send their axons to the PL at the very beginning of differentiation, perhaps even before reaching their final position. The initial axons of the neurons of the paraventricular nucleus proper (PV) appeared to reach the PL significantly later, at E17. In addition to the SO and the PV, accessory magnocellular nuclei contributed to the innervation of the PL in perinatal rats. The neurons of the retrochiasmatic accessory nucleus first sent their axons to the PL on E16-E17. Axons that originated from other accessory hypothalamic nuclei reached the PL after birth, suggesting a delay in their involvement in the regulation of visceral functions compared with other magnocellular nuclei. Thus, the axons of magnocellular neurons reach the PL unexpectedly early in embryogenesis, raising the possibility of the functional significance of vasopressin and oxytocin as fetal neurohormones.

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Early development of the hypothalamus of a wallaby (Macropus eugenii)

Cheng

Marotte

Kurths

et al. 2002

J of Comparative Neurology

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We have studied the development of the hypothalamus of an Australian marsupial, the tammar wallaby (Macropus eugenii), to provide an initial anatomic framework for future research on the developing hypothalamus of diprotodontid metatheria. Cytoarchitectural (hematoxylin and eosin), immunohistochemical (CD 15 and growth associated protein, GAP-43), tritiated thymidine autoradiography, and carbocyanine dye tracing techniques were applied. Until 12 days after birth (P12), the developing hypothalamus consisted of mainly a ventricular germinal zone with a thin marginal layer, but by P25, most hypothalamic nuclei were well differentiated, indicating that the bulk of hypothalamic cytoarchitectural development occurs between P12 and P25. Strong CD 15 immunoreactivity was found in radial glial fibers in the rostral hypothalamus during early developmental ages, separating individual hypothalamic compartments. Immunoreactivity for GAP-43 was used to reveal developing fiber bundles. The medial forebrain bundle was apparent by P0, and the fornix appeared at P12. Tritiated thymidine autoradiography revealed lateral-to-medial and dorsal-to-ventral neurogenetic gradients similar to those seen in rodents. Dye tracing showed that projections to the posterior pituitary arose from the supraoptic nucleus at P5 and from the paraventricular nucleus at P10. Projections to the medulla were first found from the lateral hypothalamic area at P0 and paraventricular nucleus at P10. In conclusion, the pattern of development of the wallaby hypothalamus is broadly similar to that found in eutheria, with comparable neurogenetic compartments to those identified in rodents. Because most hypothalamic maturation takes place after birth, wallabies provide a useful model for experimentally manipulating the developing mammalian hypothalamus.

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Vasopressin and oxytocin gene expression in intact rats and under catecholamine deficiency during ontogenesis

Cited by 23 publications

References 32 publications

Oxytocin in cardiac ontogeny

Oxytocin in cardiac ontogeny

Projections from the hypothalamus to the posterior lobe in rats during ontogenesis: 1,1?-dioctadecyl-3,3,3?,3?-tetramethylindocarbocyanine perchlorate tracing study

Early development of the hypothalamus of a wallaby (Macropus eugenii)

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