Vasomotor Activity in the Isolated Perfused Rat Liver

Bräuer, Ralph W.; Leong, G. F.; Pessotti, Rita L.

doi:10.1152/ajplegacy.1953.174.2.304

Cited by 36 publications

(4 citation statements)

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“…Before the test period the liver was control-perfused for 1 hr. to correct the anoxia caused by its isolation and to ensure that a steady state had been reached (Brauer, Leong & Pessotti, 1953). At the start of the perfusion the liver was in an anaerobic state, but after the control perfusion the lactate/pyruvate concentration ratio had decreased to that reported for blood in vivo (Hohorst, Kreutz & Bucher, 1959), which shows that the operation had been performed properly and that the flow through the liver was adequate.…”

Section: Methodsmentioning

confidence: 97%

Influence of Ethanol on the Liver Metabolism of Fed and Starved Rats

Frosander¹,

Raeihae²,

Salaspuro³

et al. 1965

Biochemical Journal

188

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1. The influence of ethanol on the metabolism of livers from fed and starved rats has been studied in liver-perfusion experiments. Results have been obtained on oxygen consumption and carbon dioxide production, on glucose release and uptake by the liver and on changes in the concentrations of lactate and pyruvate and of beta-hydroxybutyrate and acetoacetate in the perfusion medium. 2. Oxygen consumption and carbon dioxide production were lower in livers from starved rats than in livers from fed rats. Ethanol had no effect on the oxygen consumption of either type of liver. After the addition of ethanol to the perfusion medium carbon dioxide production ceased almost completely, the change being faster in livers from starved rats. 3. With livers from fed rats glucose was released from the liver into the perfusion medium. This release was slightly greater when ethanol was present. With livers from starved rats no release of glucose was observed, and when ethanol was added a marked uptake of glucose from the medium was found. A simultaneous release of glycolytic end products, lactate and pyruvate, into the medium occurred. 4. Acetate was the main metabolite accumulating in the perfusion medium when ethanol was oxidized. With livers from starved rats a slightly increased formation of ketone bodies was found when ethanol was present. 5. The lactate/pyruvate concentration ratio in the perfusion medium increased from 10 to 87 with livers from fed rats and from 20 to 171 with livers from starved rats when the livers were perfused with ethanol in the medium. The beta-hydroxybutyrate/acetoacetate concentration ratio increased from 0.8 to 7.6 with livers from fed rats and from 1.0 to 9.5 with livers from starved rats when ethanol was added to the medium. 6. The effects of ethanol are discussed and related to changes in the redox state of the liver that produce new conditions for some metabolic pathways.

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Section: Methodsmentioning

confidence: 97%

Influence of Ethanol on the Liver Metabolism of Fed and Starved Rats

Frosander¹,

Raeihae²,

Salaspuro³

et al. 1965

Biochemical Journal

188

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“…All studies were conducted during the "steady phase" of blood flow (5). Zero time is the point when the labelled material was added to the reservoir.…”

Section: Ekperime1w~almentioning

confidence: 99%

Protein Synthesis in the Perfused Rat Liver

Jensen

Tarver

1956

The Journal of General Physiology

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Several reports of investigations in which peffused rat livers were used to study plasma protein synthesis (1, 2) as well as other basic hepatic functions (3-5) suggested that these preparations offered many possibilities for the investigation of the factors involved in protein metabolism under well defined experimental conditions. Consequently, a peffusion apparatus was constructed and preliminary experiments were conducted to test the disappearance rate of Pa~-labeUed chromic phosphate in colloidal form from the perfusate. The results of a typical experiment, together with the bile flow prevailing at the same time are presented.Attention was then turned to plasma protein synthesis using L-methionine-S 85 as the tracer amino acid. The results showed that the livers were capable of rapidly incorporating this amino acid into protein which appeared in the perfusate. Regardless of the initial experimental conditions employed, however, all the specific activity curves for the protein in the perfusate approached a plateau at the end of 4 hours. Similar results have been obtained by others (1). This phenomenon could be due to either the loss of synthetic ability by the liver, or to the depletion of substrate, or to a decrease in the specific activity of the labelled amino acid. The first possibility appeared unlikely, since it was found in confirmation of other work (1) that when the incorporation was proceeding at a reduced rate the addition of more labelled amino acid resulted in an immediate increase. Consequently, studies were designed to ascertain whether the cause of this plateau was due to the rapid dilution *

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“…A weak and transient vasoconstriction was also observed in the groups perfused with rat serum or with decomplemented human serum. This phenomenon has been noticed for a long time ( Brauer et al ., 1953 ). It could be triggered by endothelium‐activating substances, either present in the peripheral blood, or released during the clotting process required to obtain the serum.…”

Section: Discussionmentioning

confidence: 61%

Reversal of the vasoconstrictor step of hyperacute xenogeneic rejection of the liver by endothelin antagonists

Zhang

Wen

Gomola

et al. 2000

British J Pharmacology

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1 The role of endothelin in the initial vasoconstrictor step of hyperacute xenogeneic rejection was investigated. 2 Isolated rat livers were perfused in recirculation. Perfusion with human sera provided an ex vivo model of hyperacute rejection in a discordant combination. 3 Perfusion of 10% xenogeneic serum induced a marked (70%) and sustained reduction of the liver ow and induced the release of endothelin into the perfusion medium. In contrast, perfusion of 10% allogeneic serum or of 10% decomplemented human serum induced a weak (25%) and transient reduction of the liver¯ow and induced the release of minimal amounts of endothelin. The simultaneous administration of BQ 123 and BQ 788, the respective antagonists of ET A and ET B endothelin receptors, or that of bosentan, a mixed ET A /ET B antagonist, antagonized the vasoconstrictor eect of 10% xenogeneic human serum, as well as that of 10 79 M endothelin-1. 4 The vasoconstrictor eects of xenogeneic serum on liver circulation are, at least partly, mediated through the release of endothelin by the graft.

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Vasomotor Activity in the Isolated Perfused Rat Liver

Cited by 36 publications

References 0 publications

Influence of Ethanol on the Liver Metabolism of Fed and Starved Rats

Influence of Ethanol on the Liver Metabolism of Fed and Starved Rats

Protein Synthesis in the Perfused Rat Liver

Reversal of the vasoconstrictor step of hyperacute xenogeneic rejection of the liver by endothelin antagonists

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