Vasoconstrictor and hemodynamic effects of a methanolic extract from Rhinella marina toad poison

Santos, Cintia Vieira Dos; Kerkhoff, Jacqueline; Tomazelli, Caroline Aparecida; Wenceslau, Camilla F; Sinhorin, Adilson P.; Rodrigues, Domingos de Jesus; Carneiro, Fernando S.; Bomfim, Gisele Facholi

doi:10.1016/j.toxicon.2022.08.018

Cited by 4 publications

(2 citation statements)

References 37 publications

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“…The volatiles profiling of RS and WS was determined using headspace SPME coupled with GC-MS, as reported in the current study, for the first time. A total of 26 volatiles were identified belonging to monoterpenes (10), aliphatic hydrocarbons (6), oxygenated compounds (4), in addition to a heterocyclic, an aromatic, and a sesquiterpene, as listed in Table 1. Due to the higher percentiles of monoterpenes and aliphatic hydrocarbons in RS compared to WS (47.82% and 37.26% vs. 25.69% and 19.76%), respectively, RS had a stronger aroma profile than WS.…”

Section: Identification Of Volatile Organic Compounds (Vocs)mentioning

confidence: 99%

Comparative MS- and NMR-Based Metabolome Mapping of Egyptian Red and White Squill Bulbs F. Liliaceae and in Relation to Their Cytotoxic Effect

Khattab

El‐Kersh

Khalifa

et al. 2023

Plants

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Urginea maritima L. (squill) species is widely spread at the Mediterranean region as two main varieties, i.e., white squill (WS) and red squill (RS), that are recognized for several health potentials. The major secondary metabolite classes of the squill are cardiac glycosides, mainly, bufadienolides, flavonoids, and anthocyanins. Herein, a multiplex MS and NMR metabolomics approach targeting secondary and aroma compounds in WS and RS was employed for varieties classification. Solid-phase micro extraction-gas chromatography/mass spectroscopy (SPME-GC/MS), ultra-high-performance liquid chromatography/mass spectrometry (UPLC/MS), as well as nuclear magnetic resonance (NMR) provided fingerprinting and structural confirmation of the major metabolites for both types of the squill. For comparison of the different platforms’ classification potential, multivariate data analysis was employed. While Bufadienolides, viz. “hydroxy-scilliglaucosidin-O-rhamnoside, desacetylscillirosidin-O-rhamnoside and bufotalidin-O-hexoside” as well as oxylipids, were enriched in WS, flavonoids, i.e., dihydro-kaempferol-O-hexoside and its aglycon, taxifolin derivative, were predominant in RS. A cytotoxicity screening against three cancer cell lines, including breast adenocarcinoma (MCF-7), lung (A-549), and ovarian (SKOV-3) cell lines was conducted. Results revealed that WS was more effective on A-549 and SKOV-3 cell lines (WS IC50 0.11 and 0.4 µg/mL, respectively) owing to its abundance of bufadienolides, while RS recorded IC50 (MCF7 cell line) 0.17 µg/mL since is is rich inflavonoids.

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Section: Identification Of Volatile Organic Compounds (Vocs)mentioning

confidence: 99%

Comparative MS- and NMR-Based Metabolome Mapping of Egyptian Red and White Squill Bulbs F. Liliaceae and in Relation to Their Cytotoxic Effect

Khattab

El‐Kersh

Khalifa

et al. 2023

Plants

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“…Experimental research studies on animals born with single ventricle would be potentially useful, but the only animal models with single ventricle available in nature are amphibians like frogs (Xenopus Laevis) and salamanders and reptiles [37][38][39][40][41][42][43][44][45][46][47][48]. Xenopus Laevis (African clawed frog) have a single ventricular chamber providing the systemic and pulmonary circulation, receiving the oxygenated blood returning from the lungs via the left atrium and the less saturated blood returning from the body via the right atrium.…”

Section: Introductionmentioning

confidence: 99%

The Functioning of the Frog’s Single Ventricle

Corno

2023

J Pediatric Adv Res.

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African clawed frog (Xenopus laevis) has double-inlet and single-outlet single ventricle supporting both systemic and pulmonary circulations, with normal lifespan of 25-30 years. The relationship between pulmonary and systemic blood flows was investigated using echocardiography. Twelve female African clawed frogs, age 18-24 months, mean weight 173±19 grams were studied. Heart rate, percutaneous oxygen saturation, echocardiography derived 2D and blood flow in all cardiovascular structures were measured in two temperature setting, 17◦C (normal environment for frogs) and 22 C. In eight frogs cerebral Near-Infra-Red Spectroscopy (NIRS) was measured. Mean oxygen saturation was 66±9% with temperature of 22◦C and 85±6% with 17◦C (P<0.001), while there was no statistical difference between mean heart rates, respectively 48 13 and 42±7 beats/min. Doppler echocardiography showed a mean total pulmonary blood flow (QP) =173.0 ml/min and mean total systemic blood flow (QS) = 99.3ml/min, with mean QP/QS of 1.74:1.00. The only statistical correlation between size of the cardiovascular structures and body weight was found for the pulmonary veins, with correlation coefficient = 0.71 (P=0.02). NIRS measurements showed a reverse correlation (coefficient = -0.21) with oxygen saturation. The frog’s heart has peculiar morphologic and functional characteristics allowing normal quality of life for many years despite the presence of single ventricle. Baseline QP/QS under general anesthesia was significant for increased total pulmonary blood flow. Exposure to environmental temperature 5◦C higher than normal caused peripheral vasodilatation, with subsequent decrease of QP/QS and reduced oxygenation.

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Toxic profile of marinobufagin from poisonous Amazon toads and antitumoral effects on human colorectal carcinomas

Ferreira

Sousa

et al. 2023

Journal of Ethnopharmacology

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Vasoconstrictor and hemodynamic effects of a methanolic extract from Rhinella marina toad poison

Cited by 4 publications

References 37 publications

Comparative MS- and NMR-Based Metabolome Mapping of Egyptian Red and White Squill Bulbs F. Liliaceae and in Relation to Their Cytotoxic Effect

Comparative MS- and NMR-Based Metabolome Mapping of Egyptian Red and White Squill Bulbs F. Liliaceae and in Relation to Their Cytotoxic Effect

The Functioning of the Frog’s Single Ventricle

Toxic profile of marinobufagin from poisonous Amazon toads and antitumoral effects on human colorectal carcinomas

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