Vasoconstrictor Activity of 8-O-Acetylharpagide from Ajuga reptans

Breschi, Maria Cristina; Martinotti, Enrica; Catalano, S.; Flamini, Guido; Morelli, Ivano; Pagni, Anna Maria

doi:10.1021/np50086a019

Cited by 31 publications

(19 citation statements)

References 6 publications

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“…In addition, the plant M. serratulum reported to possess the bioactive compounds: Triterpenoid, ursolic acid and iridoid glycoside myxopyroside they have several pharmacological activities including antimicrobial, antiasthmatic, antiallergic and anti-inflammatory, cardiovascular and anti-cancer effect (Wozniak et al, 2015;Kim et al, 2013). The potency of several iridoid derivatives on smooth muscle were reported (Chung et al, 1980;Breschi et al, 1992;Rojas et al, 2000) which were in support of our result.…”

Section: Concentration Of Extractsupporting

confidence: 90%

Bronchodilatory effect of <i>Myxopyrum serratulum</i> in animal model

Maruthamuthu

Kandasamy

2017

Bangladesh J Pharmacol

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Research ArticleBronchodilatory effect of Bronchodilatory effect of Bronchodilatory effect of Myxopy-MyxopyMyxopyrum serratulum rum serratulum rum serratulum in animal model in animal model in animal model BJP IntroductionBronchial asthma is a chronic inflammatory disease of the airway. Infiltration of various inflammatory cells such as eosinophil, macrophages and lymphocytes into the airway causes asthma and bronchitis (Patel et al., 2013).Bronchodilator drugs works by rapid reversal of the airway obstruction in asthmatics by directly acting on airway smooth muscle (Church and Hiroi, 1987). Drugs derived from natural sources are generally considered no adverse effects when compared to synthetic drugs, therefore, it might serve as better alternative for many chronic diseases such as cancer, infections and endemic diseases like asthma, bronchitis and many others (WHO, 2000). Plant extract and secondary metabolites derived from plant can directly influence the production and activation of inflammatory mediators, seconddary messengers and the expression of transcription factors (Calixto et al., 2004).Myxopyrum serratulum belongs to large shrub mainly found in Kerala at a altitude of about 600-900 m. It is commonly known as chaturamulla and traditionally, dried and powdered leaves of the plant was mixed with ghee as a remedy for asthma, cough and nerves complaints apart from which they were also used for the treatment of fever, headache and ear diseases (Wealth of India, 2011).According to earlier reports, the plant possesses antiinflammatory, antiarthritic ), antioxidant (Sheelarani et al., 2013), antipyretic (Vanughese et al., 2015, wound healing (Gopalakrishnan and Rajameena, 2013) and antimicrobial (Gopalakrishnan et al., 2012) properties.The phytochemical studies on M. serratulum revealed AbstractThe plant Myxopyrum serratulum is traditionally claimed to relieve asthma and cough. The present study was undertaken to evaluate the bronchodilatory effect of the methanolic extract of M. serratulum on histamine-induced bronchospasm by in vivo and the inhibitory effect of the extract on histaminecontracted tracheal chain and ileum by in vitro guinea pig model. Additionally, the relaxant effect of four cumulative concentrations of the extract (0.25, 0.5, 0.7 and 1.0 g%) was assessed using precontracted tracheal chain under different conditions. The extract (400 mg/kg) prolonged the preconvulsive time to 102.3 ± 3.8 sec when compared to saline and standard chlorpheniramine maleate as 121.3 ± 4.5 sec (p<0.05). The extract also possessed significant inhibitory effect on histamine-contracted guinea pig ileum and tracheal chain and also exhibited significant relaxation effect on precontracted tracheal chain of guinea pig models contracted by 60 mM KCl (p<0.001) and 10 µM methacholine (p<0.001) when compared with standard theophylline. Article Info

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Section: Concentration Of Extractsupporting

confidence: 90%

Bronchodilatory effect of <i>Myxopyrum serratulum</i> in animal model

Maruthamuthu

Kandasamy

2017

Bangladesh J Pharmacol

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“…Compounds 1, 2, 3, 4 and 5 had been already evidenced in this species (Scarpati et al 1965;Scarpati & Esposito 1967a, 1967b, Guiso et al 1974 and their presence was also confirmed in this study. Actually, these compounds and in particular harpagide (3) and its acetyl derivative (4) are considered chemotaxonomic markers for this genus and also for other related genera in the Lamiaceae family such as Ajuga (Assaad & Lahloub 1988;Breschi et al 1992), Stachys (Kotsos et al 2001;Munoz et al 2001;Háznagy-Radnai et al 2006;Venditti et al 2013aVenditti et al , 2013cVenditti et al , 2014 and Galeopsis (Wieffering & Fikenscher 1974;Venditti et al 2013b). Moreover, their occurrence may substantiate the traditional use of the plant as an anti-inflammatory agent (Guarrera 2005).…”

Section: Resultsmentioning

confidence: 98%

Reassessment of Melittis melissophyllum L. subsp. melissophyllum iridoidic fraction

Venditti

Frezza

Guarcini

et al. 2015

Natural Product Research

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The analysis of the polar fraction of Melittis melissophyllum L. subsp. melissophyllum led to the identification of several iridoid glycosides: monomelittoside (1), melittoside (2), harpagide (3), acetyl-harpagide (4) and ajugoside (5). Compounds 3 and 4 are considered marker compounds for the genus and, as well as compounds 1, 2 and 5, were already evidenced in a previous study on the nominal species. It was noteworthy of the presence of allobetonicoside (6) which was never reported for this genus. The isolation of 6 is very relevant because of its allose residue on the structure. Allose has been often found in the species of the subfamily Lamioideae even if it mostly regarded flavonoids considered of chemotaxonomical relevance for some correlated genera of Lamiaceae. Same as allosyl-glycosidic flavonoids, the presence of allosyl-glycosidic iridoids may also be an additional chemosystematic evidence of botanical relationships among Lamiaceae species and genera.

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“…The plants of the genus Ajuga have been reported to have antifungal (Anon, 2000;Kariba, 2001), antibacterial (Chen et al, 1996;Anon, 2000;Bennaghmouch et al, 2001), antimycobacterial (Cantrell et al, 1999), antihypertensive (Odek-Ogunde et al, 1993), antiplasmodial (Kuria et al, 2001(Kuria et al, , 2002, hypoglycaemic (Hilaly and Lyoussi, 2002), and larvae and insect antifeedant (Bremner et al, 1998;Bondì et al, 2000;Ben Jannet et al, 2000, 2001 activity. The empirical use of Ajuga is corroborated by the isolation and identification of a number of the active compounds, including antileukemic sterol glycosides (Akbay et al, 2002), hypoglycaemic ecdysteroids (Kutepova et al, 2001), antibacterial and insect antifeedant neo-clerodane diterpenoids (Chen et al, 1996;Bremner et al, 1998;Ben Jannet et al, 2000;Bondì et al, 2000), insect antifeedant diglyceride (Ben Jannet et al, 2001), vasoconstrictor 8-O-acetyharpagide (Breschi et al, 1992), and insect ecdysis inhibitors (Kubo et al, 1981). Some of the phytochemicals isolated from the Ajuga plants are reported to be cardiotonic (Kuria and Muriuki, 1984), renal stimulant (Aliotta and Pollio, 1994), biliary sectretagogue (Syrov et al, 1986), antidote for liver toxicity , and erythropoiesis stimulator (Syrov et al, 1997).…”

Section: Introductionmentioning

confidence: 99%