Vasoactivity of the ventral aorta of the American eel (Anguilla rostrata), Atlantic hagfish (Myxine glutinosa), and sea lamprey (Petromyzon marinus)

Evans, David H.; Harrie, Ann C.

doi:10.1002/1097-010x(20010415/30)289:5<273::aid-jez1>3.0.co;2-l

Cited by 36 publications

(10 citation statements)

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“…In teleosts, cholinergic innervations contribute to the increasing of branchial resistance, which is attenuated by the pretreatment with atropine (8), but it remains uncertain whether cholinergic nerves innervate systemic circulation and directly regulate blood pressure (42,53). Intraarterial injection of ACh increased arterial pressure in this study (data not shown) and ACh causes vasoconstriction in the eel ventral and dorsal aorta (7,20,43), although it is unclear whether the constriction is induced through the muscarinic receptor or not. Therefore, it is possible that VNP activates cholinergic system and exerts the pressor effect in eels.…”

Section: Discussionmentioning

confidence: 67%

Diversified cardiovascular actions of six homologous natriuretic peptides (ANP, BNP, VNP, CNP1, CNP3, and CNP4) in conscious eels

Nobata

Ventura

Kaiya

et al. 2010

American Journal of Physiology-Regulatory, Integrative and Comp

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The natriuretic peptide (NP) family consists of seven paralogs [atrial NP (ANP), brain NP (BNP), ventricular NP (VNP), and C-type NP 1-4 (CNP1-4)] in teleosts, but relative biological activity of the seven NPs has not been comprehensively examined using homologous peptides. In this study, we newly identified CNP3 and CNP4 in eels to use homologous peptides, but the CNP2 gene may have been silenced in this species. The CNP4 gene was expressed exclusively in the brain as CNP1, but the CNP3 gene, from which cardiac ANP, BNP, and VNP were generated by tandem duplication, was most abundantly expressed in the pituitary, suggesting its local action. All NPs induced hypotension dose dependently after intra-arterial injection with a potency order of ANP > VNP > BNP > CNP4 > CNP1 = CNP3. The degree of hypotension was similar at the ventral and dorsal aorta, indicating similar actions on the branchial and systemic circulation. The hypotension induced by cardiac NPs was longer lasting than CNPs, probably because of the difference in preferential receptors. Among cardiac NPs, the hypotensive effect of VNP lasted much longer than those of ANP and BNP, even though VNP disappeared from the blood more quickly than ANP. To analyze the unique effect of VNP, we examined possible involvement of the autonomic nervous system using ANP, VNP, and CNP3. Beta-adrenergic blockade diminished hypotensive effects of all three NPs, but alpha-adrenergic and cholinergic blockade enhanced only the effect of VNP, suggesting a specific mechanism for the VNP action. The NP-induced tachycardia was diminished by all blockers examined. Furthermore, the cardiovascular action of VNP was not impaired by a blocker of NP receptor, HS-142-1. Taken together, the homologous NPs exhibit diverse cardiovascular actions in eels partially through the autonomic nervous system, and the unique VNP action may be mediated by a novel receptor that has not been identified in teleosts.

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Section: Discussionmentioning

confidence: 67%

Diversified cardiovascular actions of six homologous natriuretic peptides (ANP, BNP, VNP, CNP1, CNP3, and CNP4) in conscious eels

Nobata

Ventura

Kaiya

et al. 2010

American Journal of Physiology-Regulatory, Integrative and Comp

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“…Desert Island Biological Laboratory, Salsbury Cove, ME for at least one week before use. They had been captured in the Union River (near Bangor, Maine) during the months of July and August, 1997August, , 1998August, , and 2001 Eels were anesthetized in 0.1% MS-222 (Sigma Chemical) in sea water, double-pithed after cervical transection, and the BA was removed by dissection; cross-sectional rings were mounted in 10 ml of teleost Ringer's solution (mM/l: NaCl, 148; NaHCO 3 , 14.9; NaH 2 PO 4 , 2.7; KCl, 2.6; CaCl 2 , 1.26; MgSO 4 , 1.24; Glucose, 5, bubbled with 1% CO 2 in air, pH¼7.8) in organ baths and maintained at 121C as described previously (Evans et al, '89;Evans and Gunderson, '98;Evans and Harrie, 2001). Initial tensions were maintained at ca.…”

Section: Methodsmentioning

confidence: 99%

“…Initial experiments tested the sensitivity of the BA rings to acetylcholine (10 -4 M), followed by the sequential addition of the following: ET-1 (10 À7 M), SNP (10 À4 M), NO (4.2 Â 10 À6 M), eANP (10 À7 M), CPR (10 À6 M), and PGE 1 (10 À6 M) to a given ring. Specific concentrations of all agonists were chosen because our earlier studies had determined that they produced significant responses in eel aortic rings (Evans and Harrie, 2001). Subsequent experiments were designed to determine the concentration dependence of the effects of some of the agonists, as well as to begin to characterize the specific receptor involved.…”

Section: Methodsmentioning

confidence: 99%

“…These studies suggest that at least the teleost BA is controlled by a variety of neurotransmitters and paracrines and, therefore, may be more than a simple windkessel. To test this hypothesis, we have examined the responses of the BA from the American eel, Anguilla rostrata, to a suite of putative signaling agents that our previous studies have found to be vasoactive in ventral aorta in this species (Evans and Harrie, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the effects of vasoactive substances on the bulbus arteriosus of the eel, Anguilla rostrata

2003

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The fish bulbus arteriosus (BA) smoothes cardiac output by expanding during cardiac systole and rebounding during diastole, thereby providing constant perfusion of the gills downstream. Published data have demonstrated innervation of the teleost BA and shown that the tension and compliance of the BA responded to vasoactive agonists, such as epinephrine and acetylcholine, suggesting that the BA was more than a mere ''windkessel.'' To examine vasoactivity in the BA more directly, we measured the responses of isolated tissue rings from the BA of the eel, Anguilla rostrata to a suite of putative vasoactive agonists, which had been shown to affect vascular smooth muscle in a variety of teleosts. The BA of the eel was insensitive to acetylcholine but constricted when endothelin (ET-1) was applied. Nitric oxide, sodium nitroprusside (SNP; NO donor), natriuretic peptides (NP), and prostaglandin E 1 (but not the prostacyclin agonist carbaprostacyclin) produced significant dilation in the BA. Since both ET-1 and sarafotoxin S6c produced concentration-dependent constriction, it appears that endothelin receptor B-type (ET B ) receptors (and possibly ET A receptors) are present. The dilation produced by SNP was also concentration dependent, as were the dilations produced by porcine C-type natriuretic peptide, eel atrial natriuretic peptide (NP receptor agonists), Sulprostone and Butaprost (PGE receptor agonists). Our data demonstrate that the BA of eel is responsive to a variety of vasoactive agonists, suggesting that the BA is under neurohumoral control. The role of agonist-induced changes in BA tension in fish cardiovascular physiology remains to be determined, as do the specific receptor types involved.

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“…Stimulation of b-adrenergic receptors also increased heart rate and pulse pressure in lamprey (Lignon 1979) via the modulation of intracellular calcium availability . In isolated lamprey aortic ring constricted by endothelin, and prostaglandin but not nitric oxide produced significant vasodilation (Evans & Harrie 2001). Based on available information, direct comparison between the angiotensin effect in bird and lamprey is inappropriate because of the intrinsic differences of their cardiovascular systems.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a native angiotensin from an anciently diverged serine protease inhibitor in lamprey

Wong

Takei²

2011

Journal of Endocrinology

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Angiotensinogen belongs to family A serine protease inhibitors (SERPIN family) and we have cloned and characterized SERPIN genes in two lamprey species, which possess all the properties of angiotensinogen. The putative angiotensinogens in lampreys can be considered as an evolutionary link between SERPIN and other angiotensinogen according to the phylogenetic analyses. The inferred sea lamprey angiotensinogen gene was expressed abundantly in liver and to a lesser extent in other tissues. The predicted lamprey angiotensin I (Ang I) sequence was unique and different from the teleost-type Ang I identified previously by the incubation of lamprey plasma with its kidney extract. Therefore, we characterized and compared the biochemical and physiological properties of this native lamprey Ang II (LpAng II) (EEDYDERPYMQPF) with teleost-type Ang II (NRVYVHPF). Using a newly developed RIA for LpAng II, plasma levels in Japanese lamprey were measured (157 . 4G35 . 2 fmol/ml, nZ6), but teleost-type Ang II was undetectable. In conscious cannulated lamprey, LpAng II at 100 pmol/kg elicited a transient vasodepressor effect. At doses higher than 300 pmol/kg, a biphasic cardiovascular response with an initial vasodepressor effect followed by a transient rebound vasopressor effect was observed in a dose-dependent manner. However, teleosttype Ang II was not vasoactive up to 1 nmol/kg. In Japanese eel, LpAng II injection up to 3 nmol/kg did not alter the cardiovascular parameters. Our results suggested that the renin-angiotensin system first appeared in cyclostomes, and LpAng II could be important for the regulation of cardiovascular dynamics in lampreys because of its potent and acute vasoactive effect.

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Vasoactivity of the ventral aorta of the American eel (Anguilla rostrata), Atlantic hagfish (Myxine glutinosa), and sea lamprey (Petromyzon marinus)

Cited by 36 publications

References 56 publications

Diversified cardiovascular actions of six homologous natriuretic peptides (ANP, BNP, VNP, CNP1, CNP3, and CNP4) in conscious eels

Diversified cardiovascular actions of six homologous natriuretic peptides (ANP, BNP, VNP, CNP1, CNP3, and CNP4) in conscious eels

Characterization of the effects of vasoactive substances on the bulbus arteriosus of the eel, Anguilla rostrata

Characterization of a native angiotensin from an anciently diverged serine protease inhibitor in lamprey

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