“…It seems that, in the past, the isolation of water-soluble compounds was more extensively performed than currently, possibly because during the golden age of antibiotics finding microbial antibiotics and other bioactive metabolites in hydrophilic fractions was frequent. 31,32,37–43 With new antibiotics discovery being increasingly more difficult, in particular antibiotics with new mechanisms-of-action, the emphasis on the isolation of water-soluble compounds has probably decreased.…”