Vasoactive intestinal polypeptide (VIP) stimulates adenylate cyclase in selected areas of rat brain

Borghi, C.; Nicosia, S.; Giachetti, Antonio; Said, Sami I.

doi:10.1016/0024-3205(79)90281-9

Cited by 78 publications

(32 citation statements)

References 11 publications

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“…Thus, VIP ontogeny in brain is in good agree¬ ment with the observations which have led to suggest that VIP is a neuromediator: presence of VIP in nerve endings (Larsson et al 1976a;Fuxe et al 1977), presence of large amount of VIP in the synaptosomal fractions of rat brain (Giachetti et al 1976(Giachetti et al , 1977Besson et al 1979), release of VIP from the synaptosomes by potassium under the dependence of calcium (Emson et al 1978), exist¬ ence of a VIP-sensitive adenylate cyclase in synap¬ tosomal fractions (Deschodt-Lanckman et al 1977;Borghi et al 1979) and specific receptors for VIP in the nerve endings (Robberecht et al 1978). The wide-spread distribution of VIP of the brain (Bes¬ son et al 1979) and its remarkable rise after birth may suggest that VIP has some general effects, which are necessary for the elaboration of various brain functions appearing after birth.…”

Section: Discussionsupporting

confidence: 75%

Ontogeny and immunoreactive forms of vasoactive intestinal peptide (VIP) in rat brain

Maletti

Besson

Bataille

et al. 1980

Acta Endocrinologica

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Immunoreactive vasoactive intestinal peptide (IR-VIP) was assayed in rat brain between day 17 postcoitum (pc) and day 60 post-partum (pp). IR-VIP was detectable at day 17 pc (0.1 \ m=+-\0.01 pmol/organ), increased up to day 20 pp (142 \m=+-\13.6 pmol/organ) and then reached a plateau up to day 60 pp (140 \m=+-\14 pmol/organ). On a concentration basis, IR-VIP increased slightly during the perinatal period and rose more sharply between day 8 pp (17.2 \m=+-\0.09 pmol/g of wet weight) and day 20 pp (105 \m=+-\10 pmol/g of wet weight).Thereafter, the concentration decreased slowly up to day 40 pp (81.2 \ m=+-\ 12 pmol/g of wet weight) and day 60 pp (69.7 \m=+-\6.9 pmol/g of wet weight). Ion-exchange chromatographic separation of extracts from rat brain obtained by two different procedures indicated the presence of two forms of VIP-like components measured in column effluents by both radioimmunoassay and radioreceptorassay. One molecular form emerged in a similar position to intestinal porcine VIP and was equally effective in radioimmunoassay and radioreceptorassay using rat liver membranes. Another component, which emerged after ion-exchange chromatography in front of porcine VIP, had on a Sephadex G-50 superfine column an elution position identical to that of intestinal porcine VIP. The immunoreactivity of the latter was significantly higher than the biological activity; it could thus be a deamidated form of VIP.

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Section: Discussionsupporting

confidence: 75%

Ontogeny and immunoreactive forms of vasoactive intestinal peptide (VIP) in rat brain

Maletti

Besson

Bataille

et al. 1980

Acta Endocrinologica

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“…In the case of VIP 6-28 the first five amino acids have been cleaved, leaving a shortened VIP molecule that is thought to bind to the VIP receptors without eliciting an effect (Fishbein et al 1994). It was reported that the antagonist VIP 6-28 neither stimulates nor inhibits VIP-induced stimulation of adenylate cyclase activity in homogenates of some areas of the rat brain (Borghi et al 1979). Thus, the cognitive effects of VIP could be associated with the (1-5) portion of the VIP molecule essential for interaction with response.…”

Section: Discussionmentioning

confidence: 97%

Lateralized hippocampal effects of vasoactive intestinal peptide on learning and memory in rats in a model of depression

Ivanova

Belcheva

et al. 2011

Psychopharmacology

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“…2). A possibly even lower sensitivity to VIP has been reported for adenylate cyclase in rat brain membranes (39). In addition, the stimulations are small (39), 25-50% over basal activity at 10 ,uM VIP in comparison to approximately 3000% at 1 IAM VIP in the brain cell culture system.…”

Section: Discussionmentioning

confidence: 98%

Regulation by secretin, vasoactive intestinal peptide, and somatostatin of cyclic AMP accumulation in cultured brain cells.

Calker¹,

Müller²,

Hamprecht³

1980

Proc. Natl. Acad. Sci. U.S.A.

102

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Secretin stimulates the accumulation of cyclic AMP (half maximally stimulating concentration: 10-20 nM) in cultured mouse brain cells mainly consisting of glioblasts. Vasoactive Various peptides are known to be common to the brain and the gastrointestinal tract and pancreas (1). Some of these peptides (e.g., substance P, somatostatin, enkephalin) have been shown to elicit specific behavioral changes or changes in specific neuron firing rates or patterns (for review see refs. 2-4). They are, therefore, hypothesized to act on neurons as neuromodulators or neurotransmitters. Some neurohormones are also known to act on glial cells by regulating the intracellular level of cyclic AMP (for review see ref. 5). The possibility had to be considered that this would not only hold for biogenic amines such as norepinephrine but also for peptide hormones. Therefore, we investigated the effects of peptide hormones on the accumulation of cyclic AMP in glial cell cultures derived from neonatal mouse brain. We report-here that somatostatin inhibits the basal and the hormone-induced accumulation of cyclic AMP in such primary cultures. Furthermore, we report that the gastrointestinal hormones secretin and vasoactive intestinal peptide (VIP) stimulate the accumulation of cyclic AMP in these cultures, probably via different types of receptors. Thus a possible role of peptide hormones in the function of glial cells is indicated. In addition, the results suggest that secretin or a closely related compound might exert regulatory influence in the brain. A preliminary report of some of these results has been presented at a conference (6).MATERIALS AND METHODS Materials. Dulbecco's modified Eagle's medium and fetal bovine serum (GIBCO) were from C. Roth, Karlsruhe, Federal Republic of Germany. A partially purified preparation of VIP (porcine) was kindly donated by V. Mutt, Stockholm, Sweden. Isolated somatostatin (cyclic; bovine) was from Beckman,

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Vasoactive intestinal polypeptide (VIP) stimulates adenylate cyclase in selected areas of rat brain

Cited by 78 publications

References 11 publications

Ontogeny and immunoreactive forms of vasoactive intestinal peptide (VIP) in rat brain

Ontogeny and immunoreactive forms of vasoactive intestinal peptide (VIP) in rat brain

Lateralized hippocampal effects of vasoactive intestinal peptide on learning and memory in rats in a model of depression

Regulation by secretin, vasoactive intestinal peptide, and somatostatin of cyclic AMP accumulation in cultured brain cells.

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