Vasoactive intestinal polypeptide and glucagon: Stimulation of adenylate cyclase activity via distinct receptors in liver and fat cell membranes

Desbuquois, Bernard; Laudat, M H; Laudat, P

doi:10.1016/0006-291x(73)90590-1

Cited by 123 publications

(36 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results obtained here together with the description of binding sites for vasoactive intestinal peptide in the preceding paper [I] support for the first time the presence of high affinity receptors for vasoactive intestinal peptide in gut epithelium and are consistent with our recent description of a adenylate cyclase sensitive to vasoactive intestinal peptide in plasma membranes prepared from intestinal epithelial cells [27]. Vasoactive intestinal peptide has also been described to act via the stimulation of adenylate cyclase in other tissues in experiments using isolated plasma membranes of liver [28,29], fat [28,29], exocrine pancreas [30] and brain [31].…”

Section: Discussionsupporting

confidence: 73%

Interaction of Vasoactive Intestinal Peptide with Isolated Intestinal Epithelial Cells from Rat

Laburthe

Amiranoff

et al. 1979

European Journal of Biochemistry

132

View full text Add to dashboard Cite

Porcine vasoactive intestinal peptide stimulated adenosine 3' : 5'-monophosphate (cyclic AMP) production in rat intestinal epithelial cells. The stimulation was dependent on time and temperature and was potentiated by the phosphodiesterase inhibitor 3-isobutyl-1 -methylxanthine. Under optimal conditions (at 15 "C, with 0.2 mM 3-isobutyl-l-methylxanthine, at a cell concentration up to 18 pg DNA/ml), the cyclic AMP production produced by vasoactive intestinal peptide was constant for 10 min and stopped after 15 min incubation, at either low (1 nM) or high (30 nM) concentration of the peptide. This plateau effect was demonstrated not to be due to an inactivation of vasoactive intestinal peptide in the medium nor to an alteration of receptors for the peptide. Cyclic AMP production was sensitive to a concentration as low as 0.1 nM vasoactive intestinal peptide. Maximal stimulation of cyclic AMP levels by vasoactive intestinal peptide was observed with 30 nM vasoactive intestinal peptide and represented an 1 1-fold increase above basal. The dose-response curve was monophasic with a K , of 2.3 x lo-' M. No cooperative effects were detected by Hill analysis. The positive non-linear relationship observed between stimulation of cyclic AMP production and occupancy of binding sites was not time-dependent as indicated by experiments performed after 15, 45 and 120 min incubation. Maximal and half-maximal responses were obtained at about 70% and 7 % occupation of binding sites, respectively.Chicken vasoactive intestinal peptide and porcine secretin were agonists of porcine vasoactive intestinal peptide with a 6-times higher and a 120-times lower potency, respectively. Among secretin analogs that were found to have low affinity for vasoactive intestinal peptide binding sites, [Calanine,secretin, that resembles vasoactive intestinal peptide at the first seven amino acids at the N-terminal end, was a partial agonist of vasoactive intestinal peptide and others failed to stimulate cyclic AMP production. Glucagon (10 pM), gastric inhibitory peptide (0.1 pM), substance P, neurotensin, octapeptide of cholecystokinin, bovine pancreatic polypeptide, human gastrin I with leucine at residue 15, Leu-enkephalin and somatostatin (1 pM) did not alter cyclic AMP levels. Non-peptide mediators such as dopamine, serotonin, acetylcholine and histamine, tested at 10 pM, were also ineffective. Prostaglandins Ez, El and isoproterenol, tested at 10 pM, induced an increase of cyclic AMP levels above basal but were 9.5, 13.7 and 17.5 times less efficient than vasoactive intestinal peptide, respectively. Thus vasoactive intestinal peptide is a unique stimulus of cyclic AMP production in rat intestinal epithelial cells.

show abstract

Section: Discussionsupporting

confidence: 73%

Interaction of Vasoactive Intestinal Peptide with Isolated Intestinal Epithelial Cells from Rat

Laburthe

Amiranoff

et al. 1979

European Journal of Biochemistry

132

View full text Add to dashboard Cite

show abstract

“…(iii) For unknown reasons the brain cells are less sensitive to secretin than the pancreatic acinar cells are. In addition, their sensitivity to VIP is far below that of pancreatic acinar cells (25,30), fat cells (31), liver (31,32), intestinal epithelium (33), pituitary (34), and brain (35,36). In these cases the VIP receptors that are highly sensitive to VIP also display a low sensitivity to secretin, as derived from receptor binding studies (32,33,35,36) and measurements of adenylate cyclase (31,34) or of concentrations of cellular cyclic AMP (33,34).…”

Section: Discussionmentioning

confidence: 99%

Regulation by secretin, vasoactive intestinal peptide, and somatostatin of cyclic AMP accumulation in cultured brain cells.

Calker¹,

Müller²,

Hamprecht³

1980

Proc. Natl. Acad. Sci. U.S.A.

102

View full text Add to dashboard Cite

Secretin stimulates the accumulation of cyclic AMP (half maximally stimulating concentration: 10-20 nM) in cultured mouse brain cells mainly consisting of glioblasts. Vasoactive Various peptides are known to be common to the brain and the gastrointestinal tract and pancreas (1). Some of these peptides (e.g., substance P, somatostatin, enkephalin) have been shown to elicit specific behavioral changes or changes in specific neuron firing rates or patterns (for review see refs. 2-4). They are, therefore, hypothesized to act on neurons as neuromodulators or neurotransmitters. Some neurohormones are also known to act on glial cells by regulating the intracellular level of cyclic AMP (for review see ref. 5). The possibility had to be considered that this would not only hold for biogenic amines such as norepinephrine but also for peptide hormones. Therefore, we investigated the effects of peptide hormones on the accumulation of cyclic AMP in glial cell cultures derived from neonatal mouse brain. We report-here that somatostatin inhibits the basal and the hormone-induced accumulation of cyclic AMP in such primary cultures. Furthermore, we report that the gastrointestinal hormones secretin and vasoactive intestinal peptide (VIP) stimulate the accumulation of cyclic AMP in these cultures, probably via different types of receptors. Thus a possible role of peptide hormones in the function of glial cells is indicated. In addition, the results suggest that secretin or a closely related compound might exert regulatory influence in the brain. A preliminary report of some of these results has been presented at a conference (6).MATERIALS AND METHODS Materials. Dulbecco's modified Eagle's medium and fetal bovine serum (GIBCO) were from C. Roth, Karlsruhe, Federal Republic of Germany. A partially purified preparation of VIP (porcine) was kindly donated by V. Mutt, Stockholm, Sweden. Isolated somatostatin (cyclic; bovine) was from Beckman,

show abstract

“…Like these two peptides, VIP has been shown to stimulate the adenylyl cyclase system in several preparations. For VIP this effect has been demonstrated in rat adipocytes (Frandsen & Moody, 1973;Desbuquois, Laudat & Laudat, 1974) rat liver membranes (Desbuquois, Laudat & Laudat, 1973), intestinal mucosa (Schwartz, Kimberg, Sheerin, Field & Said, 1974), isolated enterocytes from rat small intestine (Laburthe, Besson, Hoa & Rosselin, 1977), guinea-pig pancreatic acinar cells (Gardner, Christophe, Robberchet & Conlon, 1977), mouse pancreas homogenates, and mouse isolated islets of Langerhans (Frandsen & Moody, 1977).…”

mentioning

confidence: 96%

Vasoactive Intestinal Polypeptide Promotes Cyclic Adenosine 3‘,5’‐mono‐phosphate Accumulation in Guinea‐pig Trachea

Frandsen

Krishna

Said

1978

British J Pharmacology

View full text Add to dashboard Cite

show abstract

Vasoactive intestinal polypeptide and glucagon: Stimulation of adenylate cyclase activity via distinct receptors in liver and fat cell membranes

Cited by 123 publications

References 21 publications

Interaction of Vasoactive Intestinal Peptide with Isolated Intestinal Epithelial Cells from Rat

Interaction of Vasoactive Intestinal Peptide with Isolated Intestinal Epithelial Cells from Rat

Regulation by secretin, vasoactive intestinal peptide, and somatostatin of cyclic AMP accumulation in cultured brain cells.

Vasoactive Intestinal Polypeptide Promotes Cyclic Adenosine 3‘,5’‐mono‐phosphate Accumulation in Guinea‐pig Trachea

Contact Info

Product

Resources

About