Vasoactive Intestinal Peptide (VIP) Protects Nile Tilapia (Oreochromis niloticus) against Streptococcus agalatiae Infection

Zhang, Zhiqiang; Li, Qi; Huang, Yongxiong; Xu, Zhou; Chen, Xinjin; Jiang, Baijian; Huang, Yu; Jian, Jichang

doi:10.3390/ijms232314895

Cited by 5 publications

(6 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Myeloid differentiation factor 88 (MyD88) is a main component of MyD88‐dependent pathway, which can lead to the production of inflammatory cytokines (Gao et al, 2022). GBS infection can cause the expression of myd88 in tilapia (Zhang, Li, et al, 2022). Interestingly, medicinal plants play important roles in downregulating the expression of myd88 .…”

Section: Discussionmentioning

confidence: 99%

In vivo antibacterial activity of medicinal plant Sophora flavescens against Streptococcus agalactiae infection

Zhang

Yang²,

et al. 2023

Journal of Fish Diseases

View full text Add to dashboard Cite

Streptococcosis disease caused by Streptococcus agalactiae (Group B Streptococcus, GBS) results in a huge economic loss of tilapia culture. It is urgent to find new antimicrobial agents against streptococcosis. In this study, 20 medicinal plants were evaluated in vitro and in vivo to obtain medicinal plants and potential bioactive compounds against GBS infection. The results showed that the ethanol extracts of 20 medicinal plants had low or no antibacterial properties in vitro, with a minimal inhibitory concentration ≥256 mg/L. Interestingly, in vivo tests showed that 7 medicinal plants could significantly inhibit GBS infection in tilapia, and Sophora flavescens (SF) had the strongest anti‐GBS activity in tilapia, reaching 92.68%. SF could significantly reduce the bacterial loads of GBS in different tissues (liver, spleen and brain) of tilapia after treated with different tested concentrations (12.5, 25.0, 50.0 and 100.0 mg/kg) for 24 h. Moreover, 50 mg/kg SF could significantly improve the survival rate of GBS‐infected tilapia by inhibiting GBS replication. Furthermore, the expression of antioxidant gene cat, immune‐related gene c‐type lysozyme and anti‐inflammatory cytokine il‐10 in liver tissue of GBS‐infected tilapia significantly increased after treated with SF for 24 h. Meanwhile, SF significantly reduced the expression of immune‐related gene myd88 and pro‐inflammatory cytokines il‐8 and il‐1β in liver tissue of GBS‐infected tilapia. The negative and positive models of UPLC‐QE‐MS, respectively, identified 27 and 57 components of SF. The major components of SF extract in the negative model were α, α‐trehalose, DL‐malic acid, D‐ (−)‐fructose and xanthohumol, while in the positive model were oxymatrine, formononetin, (−)‐maackiain and xanthohumol. Interestingly, oxymatrine and xanthohumol could significantly inhibit GBS infection in tilapia. Taken together, these results suggest that SF can inhibit GBS infection in tilapia, and it has potential for the development of anti‐GBS agents.

show abstract

Section: Discussionmentioning

confidence: 99%

In vivo antibacterial activity of medicinal plant Sophora flavescens against Streptococcus agalactiae infection

Zhang

Yang²,

et al. 2023

Journal of Fish Diseases

View full text Add to dashboard Cite

show abstract

“…Treatment with VIP for 6 h increased the relative expression levels of anti-inflammatory cytokines such as IL-10 and TGF-β in different tissues. In addition, there was also a significant increase in the expression of pro-inflammatory cytokines such as IL-1β and TNF-α at 6 h of treatment in the head kidney [ 33 ]. Therefore, these studies suggest that in teleosts, VIP and ghrelin induce both pro- and anti-inflammatory responses, different from those observed in mammals.…”

Section: Discussionmentioning

confidence: 99%

“…In a study carried out in the species Paralichthys olivaceus , an increase and a decrease in VIP expression in the spleen and head kidney were observed when an in vivo challenge with Edwardsiella tarda was performed [ 32 ]. On the other hand, in research on the species Oreochromis niloticus , it was concluded that VIP would present several immunological functions, such as decreasing the expression of pro-inflammatory cytokines and increasing the expression of anti-inflammatory cytokines, reducing the expression of genes involved in inflammation signaling and protection against Streptococcus agalatiae infection [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory role of vasoactive intestinal peptide and ghrelin in Oncorhynchus mykiss

Muñoz-Flores,

Roa,

Saavedra

et al. 2023

Heliyon

View full text Add to dashboard Cite

“…For the hematoxylin and eosin (H&E) staining and immunofluorescence (IF) assay, the procedures described in our previous work ( 25 , 33 ) were followed. Briefly, the whole brains of healthy fish were isolated and fixed in Dietrich’s fixative for 24 hours.…”

Section: Methodsmentioning

confidence: 99%

Medulla oblongata and NCCs are central defenders against Streptococcus agalactiae infection of the tilapia brain

Hou,

2024

Front. Immunol.

Self Cite

View full text Add to dashboard Cite

Various types of professional immune cells first emerge in fish and likely represent the primordial form and functions. Recent advancements revealed the direct connection between the central nervous system and the immune system in the mammalian brain. However, the specifics of brain-immune networks in the fish and the underlying mechanisms of teleost’s brain against pathogen infection have not been fully elucidated. In this study, we investigated the distribution of markers representing cerebral cells associated with protection and professional lymphocytes in the seven major components of the Nile tilapia brain through RNA-Seq assay and observed the most dominant abundance in the medulla oblongata. The subsequent challenge test revealed the non-specific cytotoxic cells (NCCs) exhibited the strongest response against streptococcal infection of the brain. The presence of NCCs in the brain was then confirmed using immunofluorescence and the cytotoxic effects usually induced by NCCs under infection were determined as well. Collectively, these findings contribute significantly to comprehending the mechanism of fish neuroimmune interaction and enhancing our understanding of its evolutionary development.

show abstract

Vasoactive Intestinal Peptide (VIP) Protects Nile Tilapia (Oreochromis niloticus) against Streptococcus agalatiae Infection

Cited by 5 publications

References 57 publications

In vivo antibacterial activity of medicinal plant Sophora flavescens against Streptococcus agalactiae infection

In vivo antibacterial activity of medicinal plant Sophora flavescens against Streptococcus agalactiae infection

Immunomodulatory role of vasoactive intestinal peptide and ghrelin in Oncorhynchus mykiss

Medulla oblongata and NCCs are central defenders against Streptococcus agalactiae infection of the tilapia brain

Contact Info

Product

Resources

About