Vasoactive intestinal peptide stimulates prostate-specific antigen secretion by LNCaP prostate cancer cells

Gkonos, Peter J.; Ashby, M. H.; Andrade, Agustin

doi:10.1016/0167-0115(96)00086-9

Cited by 23 publications

(13 citation statements)

References 17 publications

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“…The cells were incubated for 2 h in serum‐free medium with several nucleotides (ATP, BzATP, UTP, and UDP at 100 μ M ) or adenosine (10 μ M ), but no increased release could be measured as compared to the control condition. On the contrary, the Vasoactive Intestinal Peptide (VIP; 100 n M ), which strongly activates AC in these cells ( Gkonos et al ., 1996 ), increased PSA secretion by about 50% (Figure 3). The secretory effect of VIP at a low concentration (1 n M ) was enhanced in a synergistic way by adenosine (10 μ M ; Figure 3) and ATP (100 μ M ), but not by UTP (data not shown).…”

Section: Resultsmentioning

confidence: 99%

“…The synthesis and secretion of PSA is under control of androgen hormones ( Lee et al ., 1995 ). However, secretion of PSA is also acutely increased by stimuli that activate AC, such as VIP ( Gkonos et al ., 1996 ). Among the three cell lines, only the LNCaP cells synthesise PSA ( Takahashi et al ., 1999 ).…”

Section: Discussionmentioning

confidence: 99%

“…The emission light (510 nm) was collected and analysed through the FL WinLab software. The ratio between the¯uorescence intensities recorded after excitation at 340 and 380 nm is related to the [Ca 2+ ] i , which was calculated according to Grynkiewicz et al (1985).…”

Section: Calcium Measurementsmentioning

confidence: 99%

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Effects of extracellular nucleotides and nucleosides on prostate carcinoma cells

Janssens

Boeynaems

2001

British J Pharmacology

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1 The purpose of this work was to characterize the receptors involved in the action of nucleotides on the human prostate carcinoma cell lines LNCaP, PC-3 and DU145. 2 Northern blotting revealed the presence of P2Y 2 , P2Y 6 and P2Y 11 messengers in the three cell lines. P2Y 1 mRNA was only observed in the DU145 cells. In both PC-3 and DU145 cells, ATP and UTP stimulated inositol phosphate accumulation in an equipotent, equiactive and non-additive way, suggesting the involvement of P2Y 2 receptors. ATP also increased cyclic AMP, but this eect is likely to result from degradation into adenosine and activation of A 2 receptor. A 2 receptor activation led to a synergistic enhancement of prostate-speci®c antigen secretion induced by vasoactive intestinal peptide. 3 RT ± PCR experiments detected the expression of the P2X 4 and P2X 5 receptors in the DU145 cells and the P2X 4 , P2X 5 and P2X 7 receptors in the PC-3 cells. The calcium in¯ux induced by BzATP con®rmed the functional expression of P2X receptors. 4 ATP inhibited the growth of PC-3 and DU145 cells. This eect was mimicked neither by UTP nor by adenosine, indicating that it does not result from phospholipase C or adenylyl cyclase activation. On the contrary, in PC-3 cells, BzATP reproduced the eect of ATP, which was associated to a moderate decrease of proliferation and an increase of apoptosis. In DU145 cells, ATP was more potent than BzATP and growth inhibition was mainly associated with necrosis. We suggest that P2X receptors might be involved in the inhibition by nucleotides of prostate carcinoma cell growth.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Effects of extracellular nucleotides and nucleosides on prostate carcinoma cells

Janssens

Boeynaems

2001

British J Pharmacology

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“…VIP increases the expression of the major angiogenic factor VEGF [14] and acts as a proangiogenic factor [15],[16],[17]. VIP increases neuroendocrine differentiation [18] and stimulates interleukin-6 production [19] and prostate-specific antigen (PSA) secretion in prostate cancer [20]. In addition, VIP stimulates HER2 transphosphorylation in androgen-independent prostate cancer cells [17], stimulates their invasive capacity [21] and contributes to prostate cancer pathogenesis by induction of malignant transformation [22].…”

Section: Introductionmentioning

confidence: 99%

Physical Activity and Natural Anti-VIP Antibodies: Potential Role in Breast and Prostate Cancer Therapy

Veljković¹,

Dopsaj²,

Dopsaj

et al. 2011

PLoS ONE

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BackgroundThere is convincing evidence from numerous clinical and epidemiological studies that physical activity can reduce the risk for breast and prostate cancer. The biological mechanisms underlying this phenomenon remain elusive. Herein we suggest a role for naturally produced antibodies reactive with the vasoactive intestinal peptide (VIP) in the suppression of breast and prostate cancer, which we believe could offer a possible molecular mechanism underlying control of these cancers by physical exercise.Methodology and ResultsWe found that sera from individuals having breast and prostate cancers have decreased titers of VIP natural antibodies as demonstrated by a lower reactivity against peptide NTM1, having similar informational and structural properties as VIP. In contrast, sera collected from elite athletes, exhibited titers of natural NTM1-reactive antibodies that are significantly increased, suggesting that physical activity boosts production of these antibodies.SignificancePresented results suggest that physical exercise stimulates production of natural anti-VIP antibodies and likely results in suppression of VIP. This, in turn, may play a protective role against breast and prostate cancers. Physical exercise should be further investigated as a potential tool in the treatment of these diseases.

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“…Moreover, VIP is involved in the proliferation and/ or differentiation of various normal and cancer cell lines [11]. In particular, VIP has been shown to potentiate the invasive capacity and the prostatic speci®c antigen (PSA) secretion in the androgenresponsive human prostatic carcinoma cell line LNCaP [12,13]. Our previous studies point out the role of VIP and PACAP in the proliferation of this tissue [14], and it is known that the expression of VPAC 1 receptors is modulated by the presence of androgen receptor in prostatic epithelial differentiation [15].…”

Section: Introductionmentioning

confidence: 99%

Vasoactive intestinal peptide (VIP) stimulates rat prostatic epithelial cell proliferation

et al. 2001

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BACKGROUND. Androgens play a major role in supporting normal growth and functional maintenance in the prostate. However, this gland contains an array of neuroendocrine peptides that can play a regulatory role in its physiopathology. Among these peptides, one of the best studied is vasoactive intestinal peptide (VIP), which is abundant in autonomic nerves surrounding both human and rat prostatic acini. This neuropeptide may act through interaction with two types of high-af®nity receptors, named VPAC 1 and VPAC 2 receptors. Another regulatory peptide, the pituitary adenylate cyclase-activating peptide (PACAP), interacts with these receptors with the same af®nity as VIP, but binds with higher af®nity to PAC 1 receptors. Human prostate tumors and rat prostate show a major presence of VPAC 1 receptors, whereas various ®ndings suggest a role for VIP in prostatic development. Here we studied the effects of VIP on the proliferation of rat prostatic epithelial cells in culture. METHODS. We studied the [ 3 H]-thymidine uptake by rat prostatic epithelial cells in culture, characterized previously by using biomarkers such as cytokeratin and vimentin. In these cells we tested the effect of VIP and PACAP-27 on two different signaling pathways, the cyclic AMP (cAMP) and the inositol phosphate (IPs). RESULTS. The rat prostatic cells in culture were cytokeratin (5,6,8) and vimentin positive, indicating that the culture was predominantly epithelial. The proliferation curves showed that the cells followed different states of growth: a quiescent, an exponential proliferative, and a steady state. Cyclic AMP production, but not inositol phosphate production, was increased in the presence of VIP and PACAP-27, which suggests the expression of VPAC 1 and/or VPAC 2 receptors primarily. VIP signi®cantly increased prostatic cell proliferation in a bimodal manner, as shown for dibutyryl cyclic AMP (dbcAMP), which suggests that the effect of VIP upon prostatic proliferation is cAMP-dependent. CONCLUSIONS. Here, we demonstrate that VIP increased [ 3 H]thymidine uptake by rat prostatic epithelial cells in culture, conceivably by the activation of the adenylate cyclase.

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Vasoactive intestinal peptide stimulates prostate-specific antigen secretion by LNCaP prostate cancer cells

Cited by 23 publications

References 17 publications

Effects of extracellular nucleotides and nucleosides on prostate carcinoma cells

Effects of extracellular nucleotides and nucleosides on prostate carcinoma cells

Physical Activity and Natural Anti-VIP Antibodies: Potential Role in Breast and Prostate Cancer Therapy

Vasoactive intestinal peptide (VIP) stimulates rat prostatic epithelial cell proliferation

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