Vasoactive Intestinal Peptide Stimulates Bone Marrow-Mesenchymal Stem Cells Osteogenesis Differentiation by Activating Wnt/β-Catenin Signaling Pathway and Promotes Rat Skull Defect Repair

Shi, Liu; Lu, Feng; Zhu, Mingzhao; Yang, Zhengmeng; Wu, Tianyi; Xu, Jia; Liu, Yang; Lin, Weiping; Lo, Jessica; Zhang, Jinfang; Li, Gang

doi:10.1089/scd.2019.0148

Cited by 49 publications

(37 citation statements)

References 56 publications

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“…Similarly, nerve axons expressing vasoactive intestinal peptide (VIP) have been observed in bone for some time ( 4 , 66 ). Recent interest in this neuropeptide secreted from the ANS has shown that it promotes osteogenic differentiation in vitro and stimulates bone repair when delivered in vivo ( 67 ). Alternately, VIP appears to be implicated in the progression of osteoarthritis through actions on subchondral bone sclerosis and vascularity ( 68 ).…”

Section: Skeletal Innervationmentioning

confidence: 99%

The Role of Nerves in Skeletal Development, Adaptation, and Aging

et al. 2020

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The skeleton is well-innervated, but only recently have the functions of this complex network in bone started to become known. Although our knowledge of skeletal sensory and sympathetic innervation is incomplete, including the specific locations and subtypes of nerves in bone, we are now able to reconcile early studies utilizing denervation models with recent work dissecting the molecular signaling between bone and nerve. In total, sensory innervation functions in bone much as it does elsewhere in the body—to sense and respond to stimuli, including mechanical loading. Similarly, sympathetic nerves regulate autonomic functions related to bone, including homeostatic remodeling and vascular tone. However, more study is required to translate our current knowledge of bone-nerve crosstalk to novel therapeutic strategies that can be effectively utilized to combat skeletal diseases, disorders of low bone mass, and age-related decreases in bone quality.

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Section: Skeletal Innervationmentioning

confidence: 99%

The Role of Nerves in Skeletal Development, Adaptation, and Aging

et al. 2020

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“…After the overexpression and knockdown, we came to the conclusion that the two of them, VIP and RPS6KA6, had a positive effect on the induction of BMSCs to form islet-like clusters with relatively high insulin secretion, while the expression of PDX1, NGN3 and NKX2.2 had similar trend, which meant that these gene might interaction with each other. According to report, VIP can modulate BMSCs osteogenesis during bone repair [57], meanwhile, more research con rm that VIP can participate in immune activities by targeting ILC3 [58,59]. Interestingly, SSTR2 may involve in the rhythmic glucagon and insulin secretion [60].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of the Transdifferentiation of canine BMSCs into Insulin Producing Cells

WANG

Dai

Zou

et al. 2020

Preprint

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Background Bone marrow mesenchymal stem cells are a potential resource for the clinical therapy of certain diseases. Canine, as a companion animal, living in the same space with human, is an ideal new model for human diseases research. Because of the high prevalence of diabetes, alternative transplantation islets resource (i.e. insulin producing cells) for diabetes treatment will be in urgent need, which makes our research on the transdifferentiation of Bone marrow mesenchymal stem cells into insulin producing cells become more important.Result In this study, we completed the transdifferentiation process and achieved the transcriptome profiling of five samples with two biological duplicates, namely, “BMSCs”, “islets”, “stage1”, “stage2” and “stage3”, and the latter three samples were achieved on the second, fifth and eighth day of induction. A total of 11,530 differentially expressed transcripts were revealed in the profiling data. The enrichment analysis of differentially expressed genes revealed several signaling pathways that are essential for regulating proliferation and transdifferentiation, including focal adhesion, ECM–receptor interaction, tight junction, protein digestion and absorption, and the Rap1 signaling pathway. Meanwhile, the obtained protein–protein interaction network and functional identification indicating involvement of three genes, SSTR2, RPS6KA6, and VIP could act as a foundation for further research.Conclusion In conclusion, to the best of our knowledge, this is the first survey of the transdifferentiation of canine BMSCs into insulin-producing cells according with the timeline using next-generation sequencing technology. The three key genes we pick out may regulate decisive genes during the development of transdifferentiation.

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“…[48,50] In addition, VIP has potential to stimulate angiogenesis within bone through enhancing endothelial tube formation as well as VEGF expression in BMSCs. [51] 2. 1…”

Section: 2 Bone Remodelingmentioning

confidence: 99%

“…[ 40 ] Acetylcholine (ACh) and vasoactive intestinal polypeptide (VIP) released by cholinergic nerves, and neuropeptide Y (NPY) produced by adrenergic nerves also play regulatory roles on bone remodeling. [ 45–49 ] The VIP boosts bone formation by downregulating the production of osteoclasts as well as promoting osteogenic differentiation of BMSCs. [ 48,50 ] In addition, VIP has potential to stimulate angiogenesis within bone through enhancing endothelial tube formation as well as VEGF expression in BMSCs.…”

Section: Communication Between Nerves and Bonementioning

confidence: 99%

Simultaneous Regeneration of Bone and Nerves Through Materials and Architectural Design: Are We There Yet?

Wan

Qin

Shen

et al. 2020

Adv Funct Materials

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Bilateral communication between bone and nerves is crucial for optimal repair of large bone defects as well as repair of peripheral nerves within the skeleton. However, simultaneous regeneration of bone and nerves is an issue that has long been overlooked in prior literature. Incongruous or even contradictory requirements or regeneration environments for bone and nerves make simultaneous regeneration of multiple tissues challenging. The present review commences with introducing natural crosstalk between bone and nerves, highlighting the rationale for simultaneous regeneration of bone and nerves. These issues will pave the way for the development of inspirational materials design concepts that capitalize on the principles of osteoconduction, osteoinduction, neuroconduction, neuroinduction, and neuroattraction. Potential strategies based on selection of appropriate scaffolds, acellular biological factors and architectural design will be addressed.

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Vasoactive Intestinal Peptide Stimulates Bone Marrow-Mesenchymal Stem Cells Osteogenesis Differentiation by Activating Wnt/β-Catenin Signaling Pathway and Promotes Rat Skull Defect Repair

Cited by 49 publications

References 56 publications

The Role of Nerves in Skeletal Development, Adaptation, and Aging

The Role of Nerves in Skeletal Development, Adaptation, and Aging

Transcriptome Analysis of the Transdifferentiation of canine BMSCs into Insulin Producing Cells

Simultaneous Regeneration of Bone and Nerves Through Materials and Architectural Design: Are We There Yet?

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