Vasoactive intestinal peptide increases intracellular free calcium in rat and human pituitary tumour cells in vitro

Prysor-Jones, R. A.; Silverlight, J. J.; Jenkins, Janis H.

doi:10.1677/joe.0.1140119

Cited by 21 publications

(7 citation statements)

References 23 publications

(27 reference statements)

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“…In the human pituitary tumour cells VIP not only increased prolactin release in vitro (Prysor-Jones et al 1987) but in the present experiments it also increased cell growth. Moreover, the effective concentrations of VIP for increased DNA synthesis were close to that of the maximum concen¬ tration found in rat portal plasma (Shimatsu, Kato, Matushita et al (1981).…”

Section: Modification Of Effects Of Fgf By Oestradiol Pdgf and Egfsupporting

confidence: 65%

“…It may well be, therefore, that there is a stimulatory interaction between oestradiol and VIP on pituitary tumours of man. In contrast with the action of VIP, it was notable that no stimulatory effect of TRH was obtained on cell growth in the same conditions in spite of its ability to release prolactin from these human cells (Prysor-Jones et al 1987). These differences in the effects on cell growth are not surprising since previous experiments have shown that the two peptides act at the cellular level in a different manner.…”

Section: Modification Of Effects Of Fgf By Oestradiol Pdgf and Egfmentioning

confidence: 95%

See 1 more Smart Citation

Oestradiol, vasoactive intestinal peptide and fibroblast growth factor in the growth of human pituitary tumour cells in vitro

Prysor-Jones

Silverlight²,

Jenkins³

1989

Journal of Endocrinology

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The growth of two human prolactin-secreting cell lines developed in our laboratory has been investigated in response to a number of factors. Oestrogen stimulated the synthesis of DNA and protein and increased prolactin secretion. Dexamethasone had the opposite effect to oestrogen. In the presence of serum, epidermal growth factor (EGF) inhibited cell growth at concentrations of 5 ng/ml. Known secretagogues of prolactin (vasoactive intestinal peptide (VIP), TRH, bombesin and neurotensin) were investigated for their action on cell growth but only VIP had a stimulatory effect. Two preparations of fibroblast growth factor (FGF) were studied. One form, derived from bovine pituitary glands, stimulated human pituitary cell growth. In contrast, another FGF, of the basic type (rFGF), was inhibitory to cell growth, increasing the time for cell doubling from 30 to 72 h. This inhibitory effect of rFGF was modified but not abolished by serum, oestradiol, platelet-derived growth factor or EGF. We conclude that bovine pituitary contains at least two fibroblast growth factors, both of which stimulate fibroblast cell growth, but one stimulates and the other inhibits human pituitary tumour cell growth.

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Section: Modification Of Effects Of Fgf By Oestradiol Pdgf and Egfsupporting

confidence: 65%

Section: Modification Of Effects Of Fgf By Oestradiol Pdgf and Egfmentioning

confidence: 95%

Oestradiol, vasoactive intestinal peptide and fibroblast growth factor in the growth of human pituitary tumour cells in vitro

Prysor-Jones

Silverlight²,

Jenkins³

1989

Journal of Endocrinology

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“…We observed that VIP caused an enhancement of Ca2 + incorporation with a peak at 5 s and lasting for 1 min. Such a finding is consistent with earlier results obtained in rat and human pituitary tumor cells indicating that VIP receptor stimu lation induces an increase of the intracellular Ca2+ concentra tion [19]. The hypothesis which could ensue from these data is that the newly incorporated Ca2+ may act as a positive modu lator of the activity of adenylate cyclase associated to VIP recep tors.…”

Section: Discussionsupporting

confidence: 92%

A Mechanism Additional to Cyclic AMP Accumulation for Vasoactive Intestinal Peptide-Induced Prolactin Release

et al. 1990

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Vasoactive intestinal peptide (VIP) is a prolactin (PRL)-releasing factor which has been proposed to exert its secreting property by activating the adenylate cyclase enzyme. The present study shows that the omission of external Ca²⁺ did not affect the ability of VIP to induce PRL release while it completely abolished the VIP stimulatory effect on adenylate cyclase. We found that VIP (500 nM) stimulated PRL secretion in a time-dependent manner reaching a plateau at 3 min. This pattern was not changed when Ca²⁺ was omitted from the incubation medium. When tested at different concentrations, VIP stimulated PRL release with EC₅₀ values of 1.3 nM in the presence of Ca²⁺ and 30 nM in the absence of Ca²⁺. On the other hand, Ca²⁺ removal completely suppressed the VIP-induced cAMP formation. VIP (200 nM) was also found to activate Ca²⁺ influx into pituitary cells. The increase in Ca²⁺ permeability showed a peak at 5 s and remained significantly higher than control values until 1 min. In conclusion, in an experimental condition where Ca²⁺ was omitted from the medium, VIP was found to induce PRL release without stimulating cAMP production. This cAMP-independent PRL release was blocked by preincubation of the cells with 1 µg/ml pertussis toxin. An additional mechanism other than adenylate cyclase activation or Ca²⁺ entry is proposed to sustain VIP-induced PRL release.

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“…Moreover, several growth factors may also modulate both cell growth and PRL production. In agreement with this notion, Elsholz and co-workers (21) (22) claimed that the effect of EGF was depen¬ dent on extracellular calcium. The plasma levels of EGF in humans (19) and mice (20) are 2-6 • 10"1() mol/1.…”

Section: Discussionmentioning

confidence: 69%

“…In agreement with Schonbrunn and co-workers (7) we have shown that EGF increases PRL synthesis and secretion in the GH4Ci cells in a dose-dependent manner. The initial increase in the [Ca2 + ]j after administration of TRH to the GH4C, cells is produced by mobilization of intracellular stores of cal¬ cium (22). Therefore the observed ED50 of ÍO^-IO-10 mol/1 suggests a physiological role for EGF in the regulation of PRL synthesis and secretion.…”

Section: Discussionmentioning

confidence: 95%

Epidermal growth factor stimulates the prolactin synthesis and secretion in rat pituitary cells in culture (GH4C1 cells) by increasing the intracellular concentration of free calcium

Aanestad¹,

Røtnes

Torjesen³

et al. 1993

Acta Endocrinologica

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Epidermal growth factor (EGF) stimulated the prolactin (PRL) synthesis and release from the GH4C1 cells in a dose-dependent manner. The ED50 was between 10−11 and 10−10 mol/l. The maximal effect was obtained at 10−9 mol/l EGF for the release, and 10−8 mol/l EGF for the synthesis. EGF stimulated the release of PRL from cell perfusion columns after a lag period of about 30 s. The maximal secretion of PRL occurred about 60 s after the start of stimulation. The PRL secretion declined to basal levels within 2 min. The EGF-stimulated PRL release was additive to the secretion evoked by thyrotropin-releasing hormone (TRH) and vasoactive intestinal peptide (VIP). An instantaneous increase in the intracellular concentration of free calcium, [Ca2+]i, of the GH4C1 cells was observed after the administration of EGF. EGF modified neither the basal nor the TRH-stimulated inositoltrisphosphate production in the GH4C1 cells, and EGF did not show any effect on the cyclic adenosine monophosphate production of these cells.

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Vasoactive intestinal peptide increases intracellular free calcium in rat and human pituitary tumour cells in vitro

Cited by 21 publications

References 23 publications

Oestradiol, vasoactive intestinal peptide and fibroblast growth factor in the growth of human pituitary tumour cells in vitro

Oestradiol, vasoactive intestinal peptide and fibroblast growth factor in the growth of human pituitary tumour cells in vitro

A Mechanism Additional to Cyclic AMP Accumulation for Vasoactive Intestinal Peptide-Induced Prolactin Release

Epidermal growth factor stimulates the prolactin synthesis and secretion in rat pituitary cells in culture (GH4C1 cells) by increasing the intracellular concentration of free calcium

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