Vasoactive intestinal peptide in bovine pulmonary artery: localisation, function and receptor autoradiography

Barnes, Peter J.; Cadieux, Alain; Carstairs, Jill R.; Greenberg, Barry; Polak, Julia M.; Rhoden, Kerry J.

doi:10.1111/j.1476-5381.1986.tb11131.x

Cited by 49 publications

(5 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Indomethacin did not affect the response to VIP, indicating that cyclooxygenase products are not involved in the observed relaxant effect. This is consistent with the finding that VIP‐induced vasodilation is rarely mediated by cyclooxygenase products (15, 35, 36). However, the finding that forskolin (an adenylate cyclase stimulator) potentiates the relaxant effect of VIP in isolated rabbit facial artery suggests that part of its effect is due to cAMP accumulation (14).…”

Section: Discussionsupporting

confidence: 92%

Influence of the endothelium on the vasorelaxant response to acetylcholine and vasoactive intestinal polypeptide in the isolated rabbit facial artery

Stojić

Radenković

Krsljak

et al. 2003

European J Oral Sciences

View full text Add to dashboard Cite

The aim was to examine the influence of the endothelium on acetylcholine (ACh) and vasoactive intestinal polypeptide (VIP) functional responses in the isolated glandular branch of rabbit facial artery precontracted with phenylephrine as well as the potential contribution of nitric oxide (NO) and prostanoids in the ACh- and VIP-induced effects. Acetylcholine caused endothelium-dependent and VIP endothelium-independent relaxations of facial artery. The effect of ACh was partly inhibited by NG-monomethyl-l-arginine (l-NMMA, a non-selective NO synthase inhibitor) or by indomethacin (a cyclooxygenase inhibitor) while being completely blocked after concomitant addition of l-NMMA and indomethacin. The relaxation of the facial artery caused by ACh was unaffected by 65 mm KCl. The VIP-induced vasodilation was potentiated by forskolin (an adenylate cyclase stimulator) and partly reduced by l-NMMA or S-methyl-l-thiocitrulline (l-SMTC, a neuronal NO synthase inhibitor), whereas it was unaffected by indomethacin. These results suggest that ACh effects on the rabbit facial artery are mediated through release of endothelium-derived NO and cyclooxygenase products, while the effect of VIP is most probably mediated by an increase of cyclic adenosine 3',5'-monophosphate (cAMP) in vascular smooth muscles and by VIP-induced release of NO from perivascular nerve fibers.

show abstract

Section: Discussionsupporting

confidence: 92%

Influence of the endothelium on the vasorelaxant response to acetylcholine and vasoactive intestinal polypeptide in the isolated rabbit facial artery

Stojić

Radenković

Krsljak

et al. 2003

European J Oral Sciences

View full text Add to dashboard Cite

show abstract

“…We studied 11 either the same or different arteries ( Figure 6). Detailed counts from the patients with diseased arteries are shown in Table 3.…”

Section: Resultsmentioning

confidence: 99%

Autoradiographic mapping of calcitonin gene-related peptide receptors in human and guinea pig hearts.

et al. 1990

View full text Add to dashboard Cite

Calcitonin gene-related peptide (CGRP) is a 37-amino acid peptide that is a potent coronary vasodilator. Although CGRP is found in high concentrations around coronary arteries, its precise function in the control of coronary vasomotor tone remains unclear. We studied the distribution of specific receptors for CGRP in guinea pig and human hearts and found that the highest concentration of specific receptors for CGRP was in the major coronary arteries, which is consistent with the hypothesis that CGRP is implicated in control of coronary vasomotor tone. Areas of coronary artery with atheroma contained significantly decreased (158+±35 grains/1,000 jLm2 tissue, n=3) binding sites compared with binding sites in normal arteries (266±10 grains/1,000 ,um2 tissue, n=11; p<0.001, t test). The decrease in receptors for CGRP around atheroma may predispose these vessels to coronary spasm. (Circulation 1990;81:741-747) C alcitonin gene-related peptide (CGRP) is a 37-amino acid peptide, the existence of which was predicted by analysis of the calcitonin gene.1 CGRP has been detected in various tissues, including the thyroid gland,2 neuroendocrine cells,3 and peripheral sensory nerves, and its physiologic importance is unknown.CGRP is an extremely potent vasodilator in animal and human vessels,4 including coronary arteries.5 Immunohistochemical studies of animal and human tissues have shown that CGRP is localized to the nerves around the coronary arteries and arterioles.6 CGRP may be detected in the circulation in picomolar concentrations,4 and infusion of CGRP into the coronary arteries causes dilatation of large and small vessels.57 However, because CGRP almost certainly has its major effects by local release from nerves, the relevance of infusion studies may be questioned. receptor-binding studies of membrane preparations do not give information about the localization of receptors, we investigated the distribution of CGRP receptors with an autoradiographic technique in human hearts removed at cardiac transplantation and in guinea pig hearts. Methods Tissue PreparationHuman coronary arteries and hearts were removed at cardiac transplantation. Five human hearts were studied, and all the patients were suffering from end-stage ischemic heart disease. The myocardial sections were taken from grossly noninfarcted tissue, and the arteries were a mixture of angiographically normal, stenosed, and occluded vessels. Guinea pig hearts were removed from freshly killed animals.The

show abstract

“…The way of VIP action on the blood vessels is only partially elucidated. Some reports suggest the direct influence of the peptide on the smooth myocythes (Barnes et al 1986), while other reports suggest indirect influence, via the endothelium-produced NO (Jovanovic et al 1998).…”

Section: Discussionmentioning

confidence: 99%

Expression of VPAC1 receptor at the level of mRNA and protein in the porcine female reproductive system

Bukowski¹,

Wąsowicz²

2015

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

The presence and distribution of vasoactive intestinal polypeptide (VIP) receptor VPAC1 was studied in the ovary, oviduct and uterus (uterine horn and cervix) of the domestic pig using methods of molecular biology (RT-PCR and immunoblot) and immunohistochemistry.The expression of VPAC1 receptor at mRNA level was confirmed with RT-PCR in all the studied parts of the porcine female reproductive system by the presence of 525 bp PCR product and at the level of proteins by the detection of 46 kDa protein band in immunoblot. Immunohistochemical stainings revealed the cellular distribution of VPAC1 receptor protein. In the ovary it was present in the wall of arterial blood vessels, as well as in the ovarian follicles of different stages. In the tubular organs the VPAC1 receptor immunohistochemical stainings were observed in the wall of the arterial blood vessels, in the muscular membrane, as well as in the mucosal epithelium.The study confirmed the presence of VPAC1 receptor in the tissues of the porcine female reproductive tract what clearly shows the possibility of influence of VIP on the porcine ovary, oviduct and uterus.

show abstract

Vasoactive intestinal peptide in bovine pulmonary artery: localisation, function and receptor autoradiography

Cited by 49 publications

References 24 publications

Influence of the endothelium on the vasorelaxant response to acetylcholine and vasoactive intestinal polypeptide in the isolated rabbit facial artery

Influence of the endothelium on the vasorelaxant response to acetylcholine and vasoactive intestinal polypeptide in the isolated rabbit facial artery

Autoradiographic mapping of calcitonin gene-related peptide receptors in human and guinea pig hearts.

Expression of VPAC1 receptor at the level of mRNA and protein in the porcine female reproductive system

Contact Info

Product

Resources

About