Vascularized neural constructs for ex-vivo reconstitution of blood-brain barrier function

Yue, Haibing; Xie, Kai; Ji, Xianglin; Xu, Bingzhe; Wang, Chong; Shi, Peng

doi:10.1016/j.biomaterials.2020.119980

Cited by 45 publications

(40 citation statements)

References 35 publications

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“…Additionally, it is well known that PCL is a hydrophobic material with low cell adhesion capacity [ 17 ]. It is therefore a common practice to coat the PCL substrates with extracellular fibers such as collagen of fibronectin or hydrogels to favor cell adhesion (both for endothelial or neural types) [ 17 ] or to condition the surface with medium containing serum proteins [ 16 ]. In the present study, the HF surfaces were not pretreated or coated that could mask plain cell–substrate interactions during adhesion.…”

Section: Resultsmentioning

confidence: 99%

“…Several works have demonstrated that poly(ε-caprolactone) (PCL) membranes can be successfully used as cell culture platforms for bio-hybrid neuronal models, small caliber blood vessel regeneration, and for the development of vascularized human hepatic tissue [13][14][15][16]. Also, a microfluidic perfusion system was recently developed using a blend of PCL and poly(dl-lactide-co-glycolide) (PLGA) made by freeze-coating a 3D-printed sacrificial template [17] as an ex vivo vascularized neural construct. Although the vasculature network of this PCL/PLGA microfluidic perfusion system could be improved to ensure a well-distributed circulatory perfusion, it recapitulated similar in vivo BBB function.…”

Section: Introductionmentioning

confidence: 99%

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Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood—Brain Barrier Models

et al. 2020

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There is a huge interest in developing novel hollow fiber (HF) membranes able to modulate neural differentiation to produce in vitro blood–brain barrier (BBB) models for biomedical and pharmaceutical research, due to the low cell-inductive properties of the polymer HFs used in current BBB models. In this work, poly(ε-caprolactone) (PCL) and composite PCL/graphene (PCL/G) HF membranes were prepared by phase inversion and were characterized in terms of mechanical, electrical, morphological, chemical, and mass transport properties. The presence of graphene in PCL/G membranes enlarged the pore size and the water flux and presented significantly higher electrical conductivity than PCL HFs. A biocompatibility assay showed that PCL/G HFs significantly increased C6 cells adhesion and differentiation towards astrocytes, which may be attributed to their higher electrical conductivity in comparison to PCL HFs. On the other hand, PCL/G membranes produced a cytotoxic effect on the endothelial cell line HUVEC presumably related with a higher production of intracellular reactive oxygen species induced by the nanomaterial in this particular cell line. These results prove the potential of PCL HF membranes to grow endothelial cells and PCL/G HF membranes to differentiate astrocytes, the two characteristic cell types that could develop in vitro BBB models in future 3D co-culture systems.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood—Brain Barrier Models

et al. 2020

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“…Millicell® Hanging Cell Culture Inserts (Corning, Inc.) were used to build an in vitro BBB model as described in a previous study (25). Briefly, bEnd.3 cells were maintained in DMEM with 10% FBS and seeded on 6-well plate at a density of 1x10 6 cells/well and incubated for 7 days at 37˚C.…”

Section: Transendothelial Migration In An In Vitro Bbb Modelmentioning

confidence: 99%

cRGDyK‑modified procaine liposome inhibits the proliferation and motility of glioma cells via the ERK/p38MAPK pathway

Gao

Yang

et al. 2021

Exp Ther Med

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Glioma is a common type of primary tumor in the central nervous system. Glioma has been increasing in incidence yearly and is a serious threat to human life and health. The aim of the present study was to prepare liposomes for enhanced penetration of the blood-brain barrier and targeting of glioma. A procaine-loaded liposome modified with the cyclic pentapeptide cRGDyK (Pro/cRGDyK-L) was designed and developed. The particle size, ζ potential, encapsulation efficiency, release profile, stability and hemolysis of Pro/cRGDyK-L were characterized in vitro. The targeting and antitumor effects of Pro/cRGDyK-L were also investigated in vitro and in vivo. The results suggested that the cRGDyK peptide significantly facilitated the ability of liposomes to transfer procaine across the BBB and improved the cellular uptake of procaine by C6 glioma cells. The results further demonstrated that Pro/cRGDyK-L strongly suppressed cell motility, stimulated apoptosis and induced cell cycle arrest. The findings further confirmed that Pro/cRGDyK-L exhibited superior antitumor effects by targeting the ERK/p38MAPK pathway and thereby suppressed tumor growth in mice. In conclusion, the present study indicated the potential of Pro/cRGDyK-L as a means to provide improved therapeutic effects on glioma through the ERK/p38MAPK pathway.

show abstract

“…Additionally, it is well known that PCL is a hydrophobic material with low cell adhesion capacity [17]. It is therefore a common practice to coat the PCL substrates with extracellular fibers such as collagen of fibronectin or hydrogels to favor cell adhesion (both for endothelial or neural types) [17] or to condition the surface with medium containing serum proteins [16].…”

Section: Cell Cultures Viability On the Hollow Fibersmentioning

confidence: 99%

“…Several works have demonstrated that poly(ε-caprolactone) (PCL) membranes can be successfully used as cell culture platform for biohybrid neuronal models, small caliber blood vessel regeneration and development of vascularized human hepatic tissue [13][14][15][16]. Also recently, a microfluidic perfusion system has been developed using a blend of PCL and poly(DL-lactide-co-glycolide) (PLGA) made by freeze-coating a 3D-printed sacrificial template [17] as an ex vivo vascularized neural construct. Although the vasculature network of this the PCL/PLGA microfluidic perfusion system could be improved to ensure a well-distributed circulatory perfusion, it recapitulated similar in vivo BBB function.…”

Section: Introductionmentioning

confidence: 99%

Novel Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop in vitro Blood-brain Barrier Models

Mantecón-Oria¹,

Diban²,

Berciano³

et al. 2020

Preprint

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There is a huge interest in developing novel hollow fiber (HF) membranes able to modulate neural differentiation to produce in vitro blood-brain barrier (BBB) models for biomedical and pharmaceutical research, due to the low cell-inductive properties of the polymer HFs used in current BBB models. In this work, poly(ε-caprolactone) (PCL) and composite PCL/graphene (PCL/G) HF membranes were prepared by phase inversion and were characterized in terms of mechanical, electrical, morphological, chemical, and mass transport properties. The presence of graphene in PCL/G membranes enlarged the pore size and the water flux and presented significantly higher electrical conductivity than PCL HFs. Biocompatibility assay showed that PCL/G HFs significantly increased C6 cells adhesion and differentiation towards astrocytes, may be attributed to their higher electrical conductivity in comparison to PCL HFs. On the other hand, PCL/G membranes produced a cytotoxic effect on the endothelial cell line HUVEC presumably related with a higher production of intracellular reactive oxygen species induced by the nanomaterial in this particular cell line. These results prove the potential of PCL HF membranes to grow endothelial cells and PCL/G HF membranes to differentiate astrocytes, the two characteristic cell types that could develop in vitro BBB models in future 3D co-culture systems.

show abstract

Vascularized neural constructs for ex-vivo reconstitution of blood-brain barrier function

Cited by 45 publications

References 35 publications

Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood—Brain Barrier Models

Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood—Brain Barrier Models

cRGDyK‑modified procaine liposome inhibits the proliferation and motility of glioma cells via the ERK/p38MAPK pathway

Novel Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop in vitro Blood-brain Barrier Models

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