Vascularized Lower Respiratory-Physiology-On-A-Chip

Nam, Hyoryung; Choi, Yoo-mi; Jang, Jinah

doi:10.3390/app10030900

Cited by 14 publications

(13 citation statements)

References 77 publications

(96 reference statements)

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“…The trachea acts as an airway to enter and exit the air during respiration. In addition, when debris, such as dust, enters into the trachea with air, it functions to move and remove the debris using ciliary movement and mucus [139,140].…”

Section: Tracheamentioning

confidence: 99%

3D Bioprinting Strategies for the Regeneration of Functional Tubular Tissues and Organs

et al. 2020

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It is difficult to fabricate tubular-shaped tissues and organs (e.g., trachea, blood vessel, and esophagus tissue) with traditional biofabrication techniques (e.g., electrospinning, cell-sheet engineering, and mold-casting) because these have complicated multiple processes. In addition, the tubular-shaped tissues and organs have their own design with target-specific mechanical and biological properties. Therefore, the customized geometrical and physiological environment is required as one of the most critical factors for functional tissue regeneration. 3D bioprinting technology has been receiving attention for the fabrication of patient-tailored and complex-shaped free-form architecture with high reproducibility and versatility. Printable biocomposite inks that can facilitate to build tissue constructs with polymeric frameworks and biochemical microenvironmental cues are also being actively developed for the reconstruction of functional tissue. In this review, we delineated the state-of-the-art of 3D bioprinting techniques specifically for tubular tissue and organ regeneration. In addition, this review described biocomposite inks, such as natural and synthetic polymers. Several described engineering approaches using 3D bioprinting techniques and biocomposite inks may offer beneficial characteristics for the physiological mimicry of human tubular tissues and organs.

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Section: Tracheamentioning

confidence: 99%

3D Bioprinting Strategies for the Regeneration of Functional Tubular Tissues and Organs

et al. 2020

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“…Therefore, several studies have employed LAB (laser-associated bioprinting) to fabricate capillary-sized microvascular networks (Arakawa et al, 2017;Miri et al, 2019). Furthermore, dynamic conditions such as the cyclic movements of alveoli and coronary arteries are also important features of the physiological environment of blood vessels, and efforts have been made to mimic these movements in vitro (Huh et al, 2010;Stucki et al, 2018;Nam et al, 2020). For instance, Grigoryan et al (2019) created a vascularized alveolar model that mimics complex microvessel networks using LAB and reproduces blood flow and oxygen delivery according to cyclic alveolar movements.…”

Section: Mimicking Physiological Characteristics Using 3d Bioprinted In Vitro Models Perfusable and Multi-layered Blood Vessel Models In mentioning

confidence: 99%

3D Bioprinting-Based Vascularized Tissue Models Mimicking Tissue-Specific Architecture and Pathophysiology for in vitro Studies

Hwang

Choi

Jang

2021

Front. Bioeng. Biotechnol.

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A wide variety of experimental models including 2D cell cultures, model organisms, and 3D in vitro models have been developed to understand pathophysiological phenomena and assess the safety and efficacy of potential therapeutics. In this sense, 3D in vitro models are an intermediate between 2D cell cultures and animal models, as they adequately reproduce 3D microenvironments and human physiology while also being controllable and reproducible. Particularly, recent advances in 3D in vitro biomimicry models, which can produce complex cell structures, shapes, and arrangements, can more similarly reflect in vivo conditions than 2D cell culture. Based on this, 3D bioprinting technology, which enables to place the desired materials in the desired locations, has been introduced to fabricate tissue models with high structural similarity to the native tissues. Therefore, this review discusses the recent developments in this field and the key features of various types of 3D-bioprinted tissues, particularly those associated with blood vessels or highly vascularized organs, such as the heart, liver, and kidney. Moreover, this review also summarizes the current state of the three categories: (1) chemical substance treatment, (2) 3D bioprinting of lesions, and (3) recapitulation of tumor microenvironments (TME) of 3D bioprinting-based disease models according to their disease modeling approach. Finally, we propose the future directions of 3D bioprinting approaches for the creation of more advanced in vitro biomimetic 3D tissues, as well as the translation of 3D bioprinted tissue models to clinical applications.

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“…The type one-collagen gel allows these cells to provide a physiologically equivalent microenvironment and actively remodel their surrounding matrices in the long-term culture and a on microfluidic device. 71 The cell-laden chip is connected with the smoking setup with a smoking machine that generates cigarette smoke in a very controllable fashion. The smoke is humidified and diluted in the mixing flask to mimic physiological concentrations in the distal part of the lung.…”

Section: Case Reports Challenges and Opportunities Of Emerging Breath...mentioning

confidence: 99%

“…The schematics in Figure also show a possibility to culture a stable and physiological stromal tissue that one can produce and connect with the device. The type one-collagen gel allows these cells to provide a physiologically equivalent microenvironment and actively remodel their surrounding matrices in the long-term culture and a on microfluidic device . The cell-laden chip is connected with the smoking setup with a smoking machine that generates cigarette smoke in a very controllable fashion.…”

Section: Case Reports Challenges and Opportunities Of Emerging Breath...mentioning

confidence: 99%

Advances in Smoking Related In Vitro Inhalation Toxicology: A Perspective Case of Challenges and Opportunities from Progresses in Lung-on-Chip Technologies

Singh

Maharjan

Kromer

et al. 2021

Chem. Res. Toxicol.

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The inhalation toxicology of multifaceted particulate matter from the environment, cigarette smoke, and e-cigarette liquid vapes is a major research topic concerning the adverse effect of these items on lung tissue. In vitro air−liquid interface (ALI) culture models hold more potential in an inhalation toxicity assessment. Apropos to e-cigarette toxicity, the multiflavor components of the vapes pose a complex experimental bottleneck. While an appropriate ALI setup has been one part of the focus to overcome this, parallel attention towards the development of an ideal exposure system has pushed the field forward. With the advent of microfluidic devices, lung-on-chip (LOC) technologies show enormous opportunities in in vitro smoke-related inhalation toxicity. In this review, we provide a framework, establish a paradigm about smoke-related inhalation toxicity testing in vitro, and give a brief overview of breathing LOC experimental design concepts. The capabilities with optimized bioengineering approaches and microfluidics and their fundamental pros and cons are presented with specific case studies. The LOC model can imitate the structural, functional, and mechanical properties of human alveolar-capillary interface and are more reliable than conventional in vitro models. Finally, we outline current perspective challenges as well as opportunities of future development to smoking lungs-on-chip technologies based on advances in soft robotics, machine learning, and bioengineering. ■ CONTENTS 1991 5. Case Reports, Challenges, and Opportunities of Emerging Breathing Lungs-on-Chip to Test Cigarettes, and e-Cigarettes Related Pathology 1994 5.a. In Vitro Inhalation Exposure with a Biomimetic Smoking Robot Compliant with Microfluidic Organ Chips 1994 5.b.

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Vascularized Lower Respiratory-Physiology-On-A-Chip

Cited by 14 publications

References 77 publications

3D Bioprinting Strategies for the Regeneration of Functional Tubular Tissues and Organs

3D Bioprinting Strategies for the Regeneration of Functional Tubular Tissues and Organs

3D Bioprinting-Based Vascularized Tissue Models Mimicking Tissue-Specific Architecture and Pathophysiology for in vitro Studies

Advances in Smoking Related In Vitro Inhalation Toxicology: A Perspective Case of Challenges and Opportunities from Progresses in Lung-on-Chip Technologies

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