Vascularization strategies of engineered tissues and their application in cardiac regeneration

Sun, Xuetao; Altalhi, Wafa; Nunes, Sara S.

doi:10.1016/j.addr.2015.06.001

Cited by 121 publications

(95 citation statements)

References 129 publications

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“…Freshly isolated SVF cells were reported to possess a superior capacity to generate in vitro microvessels compared to other prevascularization approaches based on the coculture of endothelial cells and either mesenchymal stem cells or perivascular cells [6].…”

Section: Discussionmentioning

confidence: 99%

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Control of angiogenesis and host response by modulating the cell adhesion properties of an Elastin-Like Recombinamer-based hydrogel

et al. 2017

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The control of the in vivo vascularization of engineered tissue substitutes is essential in order to obtain either a rapid induction or a complete inhibition of the process (e.g. in muscles and hyaline-cartilage, respectively). Among the several polymers available, Elastin-Like Recombinamers (ELRs)-based hydrogel stands out as a promising material for tissue engineering thanks to its viscoelastic properties, non-toxicity, and non-immunogenicity. In this study, we aimed to modulate the high angiogenic potential of adipose tissue-derived stromal vascular fraction (SVF) cells, predominantly composed of endothelial/mural and mesenchymal cells, by simply varying the cell adhesion properties of ELRs-hydrogels. Human SVF cells, embedded in RGD-REDVbioactivated or unmodified ELRs-hydrogels, were implanted in rat subcutaneous pockets either immediately or upon 5-day-culture in perfusion-bioreactors. Perfusionbased culture enhanced the endothelial cell cord-like-organization and the release of pro-angiogenic factors in functionalized constructs. While in vivo vascularization and host cell infiltration within the bioactivated gels were highly enhanced, the two processes were strongly inhibited in non-functionalized hydrogels up to 28 days. ELRsbased hydrogels showed a great potential to determine the successful integration of engineered substitutes thanks to their capacity to finely control the angiogenic/inflammation process at the recipient site, even in presence of SVF cells.

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Section: Discussionmentioning

confidence: 99%

“…myocardium) [5], whereas other tissues retain their function upon implantation only if their avascular nature is preserved in healthy condition (e.g. hyaline cartilage, cardiac valve substitute) [6][7][8]. Biomaterials alone could block or promote angiogenesis via functionalization, architecture, or composition [9].…”

Section: Introductionmentioning

confidence: 99%

Control of angiogenesis and host response by modulating the cell adhesion properties of an Elastin-Like Recombinamer-based hydrogel

et al. 2017

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“…Embryonic stem cells (ESCs) are pluripotent stem cells derived from the blastocyst stage of early mammalian embryogenesis, and are able to differentiate into any cell type [93]. As such, ESCs can differentiate and propagate, devoid of reprogramming induced genomic alterations and mutations [94].…”

Section: D Bioprinting Of the Cardiovascular Systemmentioning

confidence: 99%

“…In cardiovascular regeneration, vascularization is arguably the most important issue. The primary sources of ECs utilized experimentally are from the aorta and human umbilical vein, however, autologous cell sources such as iPSC-derived ECs or blood/bone marrow-derived endothelial progenitor cells, are more likely to be applied clinically due to their non-immunogenicity [87,93]. The source and phenotype of the ECs may also play an important role in their function, as ECs differ in functionality between different organs, but also within different parts of the heart [12,93].…”

Section: D Bioprinting Of the Cardiovascular Systemmentioning

confidence: 99%

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3D bioprinting for cardiovascular regeneration and pharmacology

Cui

Miao

Esworthy

et al. 2018

Advanced Drug Delivery Reviews

139

116

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Cardiovascular disease (CVD) is a major cause of morbidity and mortality worldwide. Compared to traditional therapeutic strategies, three-dimensional (3D) bioprinting is one of the most advanced techniques for creating complicated cardiovascular implants with biomimetic features, which are capable of recapitulating both the native physiochemical and biomechanical characteristics of the cardiovascular system. The present review provides an overview of the cardiovascular system, as well as describes the principles of, and recent advances in, 3D bioprinting cardiovascular tissues and models. Moreover, this review will focus on the applications of 3D bioprinting technology in cardiovascular repair/regeneration and pharmacological modeling, further discussing current challenges and perspectives.

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Multi‐Material Tissue Engineering Scaffold with Hierarchical Pore Architecture

Morgan

Sklaviadis

Tochka

et al. 2016

Adv Funct Materials

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Multi-material polymer scaffolds with multiscale pore architectures were characterized and tested with vascular and heart cells as part of a platform for replacing damaged heart muscle. Vascular and muscle scaffolds were constructed from a new material, poly(limonene thioether) (PLT32i), which met the design criteria of slow biodegradability, elastomeric mechanical properties, and facile processing. The vascular-parenchymal interface was a poly(glycerol sebacate) (PGS) porous membrane that met different criteria of rapid biodegradability, high oxygen permeance, and high porosity. A hierarchical architecture of primary (macroscale) and secondary (microscale) pores was created by casting the PLT32i prepolymer onto sintered spheres of poly(methyl methacrylate) (PMMA) within precisely patterned molds followed by photocuring, de-molding, and leaching out the PMMA. Pre-fabricated polymer templates were cellularized, assembled, and perfused in order to engineer spatially organized, contractile heart tissue. Structural and functional analyses showed that the primary pores guided heart cell alignment and enabled robust perfusion while the secondary pores increased heart cell retention and reduced polymer volume fraction.

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Vascularization strategies of engineered tissues and their application in cardiac regeneration

Cited by 121 publications

References 129 publications

Control of angiogenesis and host response by modulating the cell adhesion properties of an Elastin-Like Recombinamer-based hydrogel

Control of angiogenesis and host response by modulating the cell adhesion properties of an Elastin-Like Recombinamer-based hydrogel

3D bioprinting for cardiovascular regeneration and pharmacology

Multi‐Material Tissue Engineering Scaffold with Hierarchical Pore Architecture

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