Vascularization strategies for skin tissue engineering

Amirsadeghi, Armin; Jafari, Arman; Eggermont, Loek J.; Hashemi, Seyedeh‐Sara; Bencherif, Sidi A.; Khorram, Mohammad

doi:10.1039/d0bm00266f

Cited by 74 publications

(53 citation statements)

References 299 publications

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“…Uncompressed collagen hydrogels are fragile and fold when manipulated with forceps, but after plastic compression, they are significantly more stable, which improves handling. Importantly, this dermal template has been successfully used for the establishment of large DESSs (7.5 × 7.5 cm) and was eventually tested in a pig animal model [36,37,[71][72][73][74][75][76][77].…”

Section: Bioengineered Dermo-epidermal Skin Substitutes (Under Develomentioning

confidence: 99%

Bioengineered Skin Substitutes: Advances and Future Trends

Tavakoli

Klar

2021

Applied Sciences

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As the largest organ in the human body, the skin has the function of maintaining balance and protecting from external factors such as bacteria, chemicals, and temperature. If the wound does not heal in time after skin damage, it may cause infection or life-threatening complications. In particular, medical treatment of large skin defects caused by burns or trauma remains challenging. Therefore, human bioengineered skin substitutes represent an alternative approach to treat such injuries. Based on the chemical composition and scaffold material, skin substitutes can be classified into acellular or cellular grafts, as well as natural-based or synthetic skin substitutes. Further, they can be categorized as epidermal, dermal, and composite grafts, based on the skin component they contain. This review presents the common commercially available skin substitutes and their clinical use. Moreover, the choice of an appropriate hydrogel type to prepare cell-laden skin substitutes is discussed. Additionally, we present recent advances in the field of bioengineered human skin substitutes using three-dimensional (3D) bioprinting techniques. Finally, we discuss different skin substitute developments to meet different criteria for optimal wound healing.

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Section: Bioengineered Dermo-epidermal Skin Substitutes (Under Develomentioning

confidence: 99%

Bioengineered Skin Substitutes: Advances and Future Trends

Tavakoli

Klar

2021

Applied Sciences

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“…Rapid pre-vascularization based on tissue engineering is very important for the survival of transplanted scaffolds and grafts. It has been reported that an in vivo engineered pre-vascularization strategy can utilize the body itself as a bioreactor to form new blood vessels within the implanted scaffolds for skin and bone tissue regeneration, which is promising for clinical applications (21,22).…”

Section: Introductionmentioning

confidence: 99%

Acceleration of ligamentization and osseointegration processes after anterior cruciate ligament reconstruction with autologous tissue-engineered polyethylene terephthalate graft

Cai

Kang

et al. 2021

Ann Transl Med

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Background: Despite the advantages of excellent mechanical properties for rapid return to sports and early rehabilitation after anterior cruciate ligament (ACL) reconstruction with polyethylene terephthalate (PET) artificial ligament, the graft failure rate during long-term follow-up is relatively high due to poor graft-host incorporation. The purpose of the present study was to investigate the effect of autologous tissue-engineered PET (ATE-PET) grafts on osseointegration and ligamentization after ACL reconstruction.Methods: Forty-eight New Zealand white rabbits were randomly divided into PET group (n=24) and ATE-PET group (n=24). In the ATE-PET group, the rabbits initially underwent subcutaneous implantation of the PET ligament. Two weeks later, unilateral ipsilateral ACL reconstruction was performed using an ATE-PET graft. In the PET group, the rabbits underwent ACL reconstruction using PET grafts as controls.Macroscopic observation, micro-computed tomography, histological and immunofluorescent staining, and biomechanical tests were conducted to evaluate the effects at 4 and 12 weeks postoperatively. Results:The ATE-PET graft was highly pre-vascularized with myofibroblast aggregation after two weeks of subcutaneous implantation. With regard to the intraosseous part of the graft, the ATE-PET group had significantly higher bone mineral density and bone volume/total volume ratio at 12 weeks. Histologically, the width of the interface between the graft and bone was smaller. Regarding the intra-articular part, thicker tissue coverage with a glossy appearance was observed in the ATE-PET group at 12 weeks on macroscopic observation. Histological staining also showed more collagen fibers grew in the grafts with fewer inflammatory reactions of the ATE-PET group at both 4 and 12 weeks. Immunofluorescently, both α-SMA-positive vessels and α-SMA-positive myofibroblasts were found to be significantly greater around the graft in the ATE-PET group at 4 weeks and markedly declined at 12 weeks. Moreover, the ATE-PET group presented significantly greater failure load and stiffness than the PET group at 12 weeks (53.7±5.4 vs.

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“…Skin, as a superficial organ in contact with the surrounding environment, constitutes a first important guard-line against external hazards [1] . The inability to re-epithelialize wounded skin can contribute to dewatering, inflammation or even mortality [2,3] .…”

Section: Introductionmentioning

confidence: 99%

“…With the advancement of electrospinning technology, various types of electrospinning membranes, such as random, align, lattice etc., have been developed [13] . Of these, the aligned electrospinning membranes have a wide range of applications in the field of skin tissue engineering [1,14,15] , since they can provide a series of biologically chemical and physical cues for regulating cell behaviors and transmitting mechanical and electrical fields [16] . The aligned topography of electrospinning membranes has recently been of great interest due to the similarity to the arrangement of fibroblasts and smooth muscle cells in most soft tissues.…”

Section: Introductionmentioning

confidence: 99%

The Diameter Factor of Aligned Membranes Facilitates Cutaneous Wound Healing by Promoting Epithelialization in an Immune Way

Wang

Chu

Zhao

et al. 2021

Preprint

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The re-epithelialization with appendages is a major challenge to skin injury. Given that topographical factors initially modulate cell activities, the design of biomaterials with the topography (such as pattern and diameter) offers a promising strategy for achieving optimal skin wound healing. Herein, we prepare an aligned fish collagen reinforced poly (lactide-co-glycolide) electrospinning membrane (FC/PLGA) with different fiber diameters for promoting the re-epithelialization with appendages. The A300 significantly enhanced the mechanical strength and hydrophilicity, accelerated the degradation rate, and improved the cytocompatibility (proliferation and spreading) of the FC/PLGA membrane. In particular, the A300 facilitated skin wound healing (epithelization and vascularization) with the regeneration of hair follicles (HFs). The transcriptome revealed the underlying molecular mechanism that the A300 could promote macrophages transformation to reparative phenotypes (M2), subsequently exerting anti-inflammation and promoting epidermal cell migration via secreting IL10, TGFβ and MMP12. All the results indicate the A300 to be a potential candidate for guided tissue regeneration applications.

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Vascularization strategies for skin tissue engineering

Abstract: Lack of proper vascularization after skin trauma causes delayed wound healing. This has sparked the development of various tissue engineering strategies to improve vascularization.

Cited by 74 publications

References 299 publications

Bioengineered Skin Substitutes: Advances and Future Trends

Bioengineered Skin Substitutes: Advances and Future Trends

Acceleration of ligamentization and osseointegration processes after anterior cruciate ligament reconstruction with autologous tissue-engineered polyethylene terephthalate graft

The Diameter Factor of Aligned Membranes Facilitates Cutaneous Wound Healing by Promoting Epithelialization in an Immune Way

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