Vascularization in skin wound healing: where do we stand and where do we go?

Moreira, Helena; Marques, A. P.

doi:10.1016/j.copbio.2021.08.019

Cited by 61 publications

(49 citation statements)

References 78 publications

(83 reference statements)

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“…59 Vascularization is a crucial component of wound healing because it supplies nutrients during the wound repair. 60 CD31 is a marker for vascular endothelial growth factor (VEGF). When wounds were treated with Gel-Fe-MnO 2 (+NIR), more blood vessels developed in the healed skin tissue than in the Gel-Fe-MnO 2 (−NIR) group, according to the results of CD31 immunohistochemistry staining (Figure 12C).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Injectable Antibacterial Gelatin-Based Hydrogel Incorporated with Two-Dimensional Nanosheets for Multimodal Healing of Bacteria-Infected Wounds

Kalantari-Hesari

Haghi

Alinezhad³

et al. 2022

ACS Appl. Bio Mater.

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The design and development of multifunctional injectable hydrogels with high photothermal antibacterial activity and shape adaptability to accelerate bacteria-infected wound healing is of critical importance in clinical applications. In this study, a hybrid hydrogel composed of gelatin, iron, and MnO 2 nanosheets was prepared by multiple interactions, including coordinative and hydrogen bonding as well as electrostatic attraction. The introduced MnO 2 and Fe components made the hydrogels photothermally and chemodynamically active, thereby endowing them with potent antibacterial capabilities against both Gram-negative and Grampositive bacteria. Because of the Fenton activity of the hydrogels, they could produce abandoned oxygen, which is highly crucial in the healing process of wounds. They also showed good cytocompatibility and hemocompatibility as well as high hemostatic properties. Moreover, the injectable hydrogels could fill irregular wounds and significantly accelerate bacteria-infected wound healing through decreasing the inflammatory response and increasing blood vessels. These features indicated the promising potential of the multifunctional hydrogel for healing infected full-thickness wounds.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Injectable Antibacterial Gelatin-Based Hydrogel Incorporated with Two-Dimensional Nanosheets for Multimodal Healing of Bacteria-Infected Wounds

Kalantari-Hesari

Haghi

Alinezhad³

et al. 2022

ACS Appl. Bio Mater.

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“…Also, VEGF has proangiogenic activity and is influential in neovascularization and granulation tissue formation. Besides, VEGF activates the microvascular endothelial cells (ECs) and promotes fibroblast migration (53). As shown, the postbiotic film enhanced VEGF expression during the wound healing process, which was reflected in faster wound closure and healing.…”

Section: Discussionmentioning

confidence: 95%

Novel Wound Dressing Based on Postbiotic/Chitosan Film Accelerates Cutaneous Wound Healing

Jamaran

Jafari

Marjani

et al. 2022

Jundishapur J Microbiol

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Background: Wound healing is a complex and overlapping process involving immune cells, cytokines, and growth factors. Objectives: This study aimed to design and evaluate a novel wound dressing based on postbiotic/chitosan in accelerating wound healing. Methods: Lactobacillus reuteri PTCC1655 was cultured, and the cell-free supernatant (postbiotic) was obtained by medium centrifugation. The films were prepared using the solvent casting method and evaluated in terms of water absorption index, water vapor transmission rate, and antimicrobial properties. Forty-five male Wistar rats were subjected to a full-thickness excisional wound to assess the wound healing potential. The rats were randomly divided into ctrl-, chitosan, and postbiotic groups. The time-course histological and gene expression analysis was performed to compare the dressing efficacy. Results: The films showed proper water absorption and water vapor transmission rate and inhibited the pathogens commonly associated with wound infection. The postbiotic film improved wound healing by modulating the inflammatory phase, increasing collagen and elastin deposition, and enhancing angiogenesis based on the histological results. The gene expression assay showed that the postbiotic film accelerated wound healing by improving the expression of inflammatory mediators (IL-6 and TNF-α) and anti-inflammatory mediators (TGF-β and VEGF). Conclusions: The cell-free supernatant/chitosan/polyethylene glycol (CFS/CS/PEG) biodegradable film could be introduced as a novel dressing for cutaneous wound healing. This transparent film enhances cutaneous wound healing by modulating infiltrated immunity cells and expressing inflammatory/anti-inflammatory cytokines.

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“…The formation of new blood vessels represents another critical event of wound healing. This angiogenesis occurs throughout all stages of wound repair [ 58 ]. HIF-1 has been reported to be involved in the process of angiogenesis through wound healing [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Novel Nanoconjugate of Apamin and Ceftriaxone for Management of Diabetic Wounds

Alamoudi

Alharbi

Abdel-Naim

et al. 2022

Life

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Diabetic hyperglycemia delays wound healing, leading to serious consequences. Topical antibiotics can reduce the risk of a wound infection during healing; nevertheless, the microbial fight against antibiotics brings about public health challenges. Anti-microbial peptides (AMPs) belong to a novel class of drug that is used to prevent and treat systemic and topical infections. The aim of the current work was to achieve better wound healing in diabetic rats by conjugating the anti-microbial peptide “apamin” (APA) with the broad-spectrum antibiotic “ceftriaxone” (CTX) to form a nanocomplex. The CTX–APA nanoconjugate formulation was optimized using a Box–Behnken design. The optimized CTX–APA nanoconjugate formulation was evaluated for its size and zeta potential, and was then examined using transmission electron microscopy (TEM). The CTX–APA nanoconjugate was loaded onto a hydroxypropyl methylcellulose (2% w/v)-based hydrogel. It was observed that the application of the CTX–APA nanocomplex on the wounded skin of diabetic rats accelerated the regeneration of the epithelium, granulation tissue formation, epidermal proliferation, and keratinization. The nanocomplex was capable of significantly reducing the expression of tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6), while increasing the expression of transforming growth factor beta-1 (TGF-β1) as well as the angiogenic markers: hypoxia-inducible factor 1-alpha (HIF-1α) and vascular endothelial growth factor (VEGF). Conclusively, the application of an ion-paired CTX–APA nanocomplex enhances wound healing in diabetic rats.

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Vascularization in skin wound healing: where do we stand and where do we go?

Cited by 61 publications

References 78 publications

Injectable Antibacterial Gelatin-Based Hydrogel Incorporated with Two-Dimensional Nanosheets for Multimodal Healing of Bacteria-Infected Wounds

Injectable Antibacterial Gelatin-Based Hydrogel Incorporated with Two-Dimensional Nanosheets for Multimodal Healing of Bacteria-Infected Wounds

Novel Wound Dressing Based on Postbiotic/Chitosan Film Accelerates Cutaneous Wound Healing

Novel Nanoconjugate of Apamin and Ceftriaxone for Management of Diabetic Wounds

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